Silk fibroin-gelatin films crosslinked by genipin for regenerating tympanic membrane perforations

Aleemardani, Mina; Akbarnejad, Zeinab; Jalessi, Maryam; Chahsetareh, Hadi; Hajmohammadi, Zeinab; Hassanzadeh, Sajad; Najafi, Roghayeh; Alizadeh, Rafieh; Farhadi, Mohammad; Bagher, Zohreh

doi:10.1016/j.jddst.2024.105642

Cited by 1 publication

(1 citation statement)

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“…However, this procedure's utilization depleted the carboxylate anions of glutamic acid, leading to compromised cell spreading, survival, and proliferation [19,20]. Indeed, genipin cross-linked collagen has demonstrated enhanced durability [21][22][23]; however, its potential for discoloration restricts its suitability for applications in biomedicine. As a result, the quest for highly biocompatible and durable collagen fillers remains a considerable challenge in the field of biomaterials research.…”

Section: Introductionmentioning

confidence: 99%

Engineering a durable BDDE cross-linked collagen filler for skin rejuvenation

Wang,

Yan,

Zhang

et al. 2024

Biomed. Mater.

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Skin aging, characterized by reduced regeneration, chronic inflammation, and heightened skin cancer risk, poses a significant challenge. Collagen fillers have emerged as a potential solution for skin rejuvenation by stimulating collagen regeneration. However, their clinical efficacy is limited by inherent instability and vulnerability to in vivo degradation by collagenase. Chemical cross-linking presents a promising approach to enhance stability, but it carries risks such as cytotoxicity, calcification, and discoloration. Here, we introduce a highly durable 1,4-butanediol diglycidyl ether (BDDE) cross-linked collagen filler for skin rejuvenation. BDDE effectively cross-links collagen, resulting in fillers with exceptional mechanical strength and injectability. These fillers demonstrate favorable stability and durability, promoting proliferation, adhesion, and spreading of human foreskin fibroblast-1 cells in vitro. In vivo studies confirm enhanced collagen regeneration without inducing calcification. BDDE cross-linked collagen fillers offer promising prospects for medical cosmetology and tissue regeneration.

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