Silk fibroin/chitosan scaffold with tunable properties and low inflammatory response assists the differentiation of bone marrow mesenchymal stem cells

Li, Dawei; Lei, Xiaohua; He, Fang; He, Jinguang; Liu, Yali; Ye, Ya-Jing; Deng, Xudong; Duan, Enkui; Yin, Da‐Chuan

doi:10.1016/j.ijbiomac.2017.07.080

Cited by 64 publications

(35 citation statements)

References 65 publications

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“…Therefore, only SF scaffolds rather than hydrogels with various differentiation capacities for MSCs have been reported in several studies. 56–59 As far as we know, this is the first time tunable differentiation for the growth factor free SF hydrogels was found. More interestingly, the differentiation behaviors of the stem cells on our SF hydrogels are consistent with that cultured on other biomaterial hydrogels.…”

Section: Resultsmentioning

confidence: 68%

Bioactive silk hydrogels with tunable mechanical properties

et al. 2018

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Developing bioactive hydrogels with potential to guide the differentiation behavior of stem cells has become increasingly important in the biomaterials field. Here, silk hydrogels with tunable mechanical properties were developed by introducing inert silk fibroin nanofibers (SNF) within an enzyme crosslinked system of regenerated silk fibroin (RSF). After the crosslinking reaction of RSF, the inert SNF was embedded into the RSF hydrogel matrix, resulting in improved mechanical properties. Tunable stiffness in the range of 9–60 KPa was achieved by adjusting the amount of the added NSF, significantly higher than SNF-free hydrogels formed under same conditions (about 1 KPa). In addition, the proliferation of rat bone marrow derived mesenchymal stem cells cultured on the composite hydrogels and differentiated into endothelial cells, myoblast and osteoblast cells was improved, putatively due to the control of stiffness of the hydrogels. Bioactive and tunable silk-based hydrogels were prepared via a composite SNF and crosslinked RSF system, providing a new strategy to design silk biomaterials with tunable mechanical and biological performance.

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Section: Resultsmentioning

confidence: 68%

Bioactive silk hydrogels with tunable mechanical properties

et al. 2018

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“…The rBM-MSCs was isolated and characterized as described in supporting information. 25 The metabolic activity of cells was evaluated by MTT method, and the absorbance at 490 nm was directly related to the total metabolic activity of the living cells. The MTT absorbance of cells cultured on TCP steadily increased and reached the maximum at day 21 (Figure 3).…”

Section: Culture Of Rbm-mscsmentioning

confidence: 99%

“…In our previous work, SF/CS scaffold have been developed to support the growth, proliferation, osteogenic and adipogenic differentiation of BM-MSCs. 25 However, the effect of SF/CS thin film on BM-MSCs has not been reported before, especially on the induction of adipogenic differentiation.…”

Section: Introductionmentioning

confidence: 98%

Silk fibroin/chitosan thin film promotes osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells

et al. 2018

J Biomater Appl

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As a biodegradable polymer thin film, silk fibroin/chitosan composite film overcomes the defects of pure silk fibroin and chitosan films, respectively, and shows remarkable biocompatibility, appropriate hydrophilicity and mechanical properties. Silk fibroin/chitosan thin film can be used not only as metal implant coating for bone injury repair, but also as tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair. However, the biocompatibility of silk fibroin/chitosan thin film for mesenchymal stem cells, a kind of important seed cell of tissue engineering and regenerative medicine, is rarely reported. In this study, silk fibroin/chitosan film was prepared by solvent casting method, and the rat bone marrow-derived mesenchymal stem cells were cultured on the silk fibroin/chitosan thin film. Osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells were induced, respectively. The proliferation ability, osteogenic and adipogenic differentiation abilities of rat bone marrow-derived mesenchymal stem cells were systematically compared between silk fibroin/chitosan thin film and polystyrene tissue culture plates. The results showed that silk fibroin/chitosan thin film not only provided a comparable environment for the growth and proliferation of rat bone marrow-derived mesenchymal stem cells but also promoted their osteogenic and adipogenic differentiation. This work provided information of rat bone marrow-derived mesenchymal stem cells behavior on silk fibroin/chitosan thin film and extended the application of silk fibroin/chitosan thin film. Based on the results, we suggested that the silk fibroin/chitosan thin film could be a promising material for tissue engineering of bone, cartilage, adipose, and skin.

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“…Silk fibroin (SF) is the widely used biomaterial due to its favorable biological compatibility, biodegradability, and mechanical strength [1]. In recent years, SF has been blended with polysaccharides such as chitosan, hyaluronic acid, alginate and k-carragenan for various biomedical applications [2][3][4][5]. The mixing of polysaccharide to SF imparts a certain degree of elasticity, softness, and hydrophilicity to the blended material.…”

Section: Introductionmentioning

confidence: 99%

Preparation of Drug Eluting Natural Composite Scaffold Using Response Surface Methodology and Artificial Neural Network Approach

Shera

Sahu

Banik

2018

Tissue Eng Regen Med

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Silk fibroin/xanthan composite was investigated as a suitable biomedical material for controlled drug delivery, and blending ratios of silk fibroin and xanthan were optimized by response surface methodology (RSM) and artificial neural network (ANN) approach. A non-linear ANN model was developed to predict the effect of blending ratios, percentage swelling and porosity of composite material on cumulative percentage release. The efficiency of RSM was assessed against ANN and it was found that ANN is better in optimizing and modeling studies for the fabrication of the composite material. In-vitro release studies of the loaded drug chloramphenicol showed that the optimum composite scaffold was able to minimize burst release of drug and was followed by controlled release for 5 days. Mechanistic study of release revealed that the drug release process is diffusion controlled. Moreover, during tissue engineering application, investigation of release pattern of incorporated bioactive agent is beneficial to predict, control and monitor cellular response of growing tissues. This work also presented a novel insight into usage of various drug release model to predict material properties. Based on the goodness of fit of the model, Korsmeyer-Peppas was found to agree well with experimental drug release profile, which indicated that the fabricated material has swellable nature. The chloramphenicol (CHL) loaded scaffold showed better efficacy against gram positive and gram negative bacteria. CHL loaded SFX55 (50:50) scaffold shows promising biocomposite for drug delivery and tissue engineering applications.

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Silk fibroin/chitosan scaffold with tunable properties and low inflammatory response assists the differentiation of bone marrow mesenchymal stem cells

Cited by 64 publications

References 65 publications

Bioactive silk hydrogels with tunable mechanical properties

Bioactive silk hydrogels with tunable mechanical properties

Silk fibroin/chitosan thin film promotes osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells

Preparation of Drug Eluting Natural Composite Scaffold Using Response Surface Methodology and Artificial Neural Network Approach

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