Silk fibroin/cellulose blend films: Preparation, structure, and physical properties

Freddi, Giuliano; Romanó, Maria; Massafra, Maria Rosaria; Tsukada, Masaru

doi:10.1002/app.1995.070561203

Cited by 141 publications

(104 citation statements)

References 23 publications

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“…By blending with other natural or synthetic polymers, such as cellulose (Freddi et al, 1995) or chitosan (Chen et al, 1997), silk fibroin can be processed into films or fiber silk fibroin scaffolds for a number of tissue engineering applications in skeletal tissues like bone and ligaments, and in connective tissues like skin. Moreover, SF films, fibers and gels have been investigated in vitro with different cell (Unger et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Cytocompatibility of regenerated silk fibroin film: a medical biomaterial applicable to wound healing

Liu

Jiang

Sheng

et al. 2010

J. Zhejiang Univ. Sci. B

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Abstract:Objective: To explore the feasibility of using regenerated silk fibroin membrane to construct artificial skin substitutes for wound healing, it is necessary to evaluate its cytocompatibility. Methods: The effects of regenerated silk fibroin film on cytotoxicity, adhesion, cell cycle, and apoptosis of L929 cells, growth and vascular endothelial growth factor (VEGF) expression of ECV304 cells, and VEGF, angiopoietin-1 (Ang-1), platelet-derived growth factor (PDGF) and fibroblast growth factor 2 (FGF2) expression of WI-38 cells were assessed by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay, viable cell counting, flow cytometry (FCM), and enzyme-linked immunosorbant assay (ELISA). Results: We showed that the regenerated silk fibroin film was not cytotoxic to L929 cells and had no adverse influence on their adhesion, cell cycle or apoptosis; it had no adverse influence on the growth and VEGF secretion of ECV304 cells and no effect on the secretion of VEGF, Ang-1, PDGF and FGF2 by WI-38 cells. Conclusion: The regenerated silk fibroin film should be an excellent biomaterial with good cytocompatibility, providing a framework for reparation after trauma in clinical applications.

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Section: Discussionmentioning

confidence: 99%

Cytocompatibility of regenerated silk fibroin film: a medical biomaterial applicable to wound healing

Liu

Jiang

Sheng

et al. 2010

J. Zhejiang Univ. Sci. B

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“…However, uniqueness of SF is believed to arise from the glycilalanil (GlyAla) repeats, which tend to form the b structure under certain conditions and it can be easily modified by several treatments such as heating, immersion in methanol, shearing, blending with natural polymers such as sodium alginate [18], cellulose [19] and chitosan [20], or synthetic polymers such as poly(acrylamide) [21], poly(vinyl alcohol) [22], poly(ethylene glycol) [23] and poly(ethylene oxide) [24], or introducing chemical crosslinking agents [17].…”

Section: Introductionmentioning

confidence: 99%

Silk fibroin as a novel coating material for controlled release of theophylline

Bayraktar

Malay

Özgarip

et al. 2005

European Journal of Pharmaceutics and Biopharmaceutics

111

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The aim of this study was to explore potential use of the silk fibroin (SF) as an aqueous coating material for theophylline tablets. We have examined the film forming and coating properties of heat-treated fibroin, SF solution having different amounts of polyethylene glycol (PEG) and 1-ethyl-3-(3-dimethyl aminopropyl)carbodiimide (EDC) cross-linked SF. Heat-treated SF material possessed a brittle structure, which resulted in poor film forming and coating properties. The optimum PEG amount in SF solution was determined as 17% (by weight) for an acceptable film forming and zero order release profile. EDC cross-linked SF has shown a very good film forming and coating property with a potential for sustaining the drug release from coated theophylline tablets. Dissolution data for coated theophylline tablets were analyzed using Ritger and Peppas equation to describe the mechanism of drug release. Drug release from the EDC coated tablets followed zero-order kinetics. Release rate constants were found to be 0.26, 0.19, 0.16% min K1 for single-coated, double coated, and triple coated tablets, respectively. These results clearly demonstrated that silk fibroin has high utility as a novel aqueous coating material for controlled release products. q

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“…In the past two decades, materials concerning with SF had also been utilized in the areas of biomedical and biochemical applications. 1 -3 Blends of SF with other natural or synthetic polymers, such as poly(vinyl alcohol) (PVA), 4 -6 chitosan, 7 sodium polyglutamate,8 sodium alginate 9 and cellulose 10 have attracted abundant academic and practical interest. It is anticipated that such materials show new desirable properties not to be expected in conventional homopolymers.…”

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confidence: 99%

Complex Formation of Silk Fibroin with Poly(acrylic acid)

Gao

Shao

Sun

et al. 2000

Polym J

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ABSTRACT:Silk fibroin (SF) and poly(acrylic acid) (PAA) can interact when their aqueous solutions were mixed. Viscometric study showed that SF/PAA complex formation depended on the ratios of SF and PAA in dilute solution. SF/ PAA complexes varied from soluble state to gel and to precipitate when both concentrations of mixed solution were increased gradually. FT-IR results showed that PAA and SF were liable to form interpolymer hydrogen bonds, and SF conformation changed in the complexes. FT-IR spectra also suggested the formation and crystal perfection of PAA/SF complex crystal relied on the ratios of SF and PAA, which were confirmed by the observation of X-ray diffraction.KEY WORDS Complex/ Silk Fibroin I Poly(acrylic acid) I Hydrogen Bond I Silk fibroin (SF) fibers from Bombyx mori silkworm have primarily been used in textile industry for thousands of years. In the past two decades, materials concerning with SF had also been utilized in the areas of biomedical and biochemical applications. 1 -3 Blends of SF with other natural or synthetic polymers, such as poly(vinyl alcohol) (PVA), The complexation of PAA with poly(amino acids), such as poly (L-lysine) (PLL) 24 · 25 and poly(L-proline) (PLP), 26 was also invested, and interpolymer interaction between PAA and poly(amino acids) caused the conformation change ofpoly(amino acids).Recently, we have been interested in various acrylic polymers and SF blends. In our previous articles, it was found that SF was immiscible with polyacrylonitrile, but was partly miscible with poly(acrylonitrile-co-methyl acrylate) (PANMA). 27 · 28 Because of the formation of hydrogen bonds between SF and P ANMA, conformation change of SF in their blends was observed. In systems of SF/poly(potassium acrylic acid) (PAA-K) blend as well as SF/polyalanine (PALA) blend, 29 the specific interaction and conformation change of SF in these blends were observed as well. In order to provide further evidence on the specific interactions and conformation change of SF in blends, we explored the blend of SF/PAA system in present work. SF/PAA complex forming by hydrogen bond could be detected with viscometric, FT-IR and Xray diffraction. The crystallinity and perfection of complex crystal were both dependent on SF/PAA ratios. EXPERIMENTAL MaterialsPreparation of SF solution : Degummed SF from Bombyx mori silk fibers was dissolved in 9.3 mol L -l LiBr aqueous solution under constant stirring for several hours at room temperature. 6 After dialysis against deionized water for three days, the solution was filtered. This solution was further diluted with deionized water to prepare the required concentration.Preparation of PAA solution: PAA was synthesized according to the reference, 30 and molecular weight, as determined by intrinsic viscosity from the equation, 31 [TJ] =8.5 X 10-4 M 112 , is 35000. Then PAA aqueous solutions ofrequired concentration were prepared.Preparation of dilute SF/PAA mixture solution: mixed solutions were prepared by dropwise adding required amount ofO.1 wt% PAA into 0.1 wt% SF s...

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Silk fibroin/cellulose blend films: Preparation, structure, and physical properties

Cited by 141 publications

References 23 publications

Cytocompatibility of regenerated silk fibroin film: a medical biomaterial applicable to wound healing

Cytocompatibility of regenerated silk fibroin film: a medical biomaterial applicable to wound healing

Silk fibroin as a novel coating material for controlled release of theophylline

Complex Formation of Silk Fibroin with Poly(acrylic acid)

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