Silk fibroin as a vehicle for drug delivery applications

Wenk, Esther; Merkle, Hans P.; Meinel, Lorenz

doi:10.1016/j.jconrel.2010.11.007

Cited by 468 publications

(408 citation statements)

References 150 publications

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“…In this work, we added CS to SF to reconstruct a 3D scaffold instead of corneal stroma for the first time. Treatment of SFCS scaffolds with methanol and sodium hydroxide resulted in a more compact, but otherwise unaffected sheet structure [Wenk et al, 2011], which is a first step towards mimicking the layered structure of the basement membrane. Another important aspect of extracellular matrix (ECM) is its ability to store water, which supports various cellular activities and functions.…”

Section: Discussionmentioning

confidence: 99%

Use of a Silk Fibroin-Chitosan Scaffold to Construct a Tissue-Engineered Corneal Stroma

Guan

Tang

et al. 2013

Cells Tissues Organs

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Purpose: To construct a scaffold using silk fibroin (SF) and chitosan (CS) that could replace the corneal stroma with the biological characteristics of the scaffold materials still intact. Methods: To develop an organotypic corneal stroma, SF and CS were chosen to synthesise the tissue-engineered bioscaffold. We cultured primary rabbit corneal epithelial cells and corneal stromal cells in vitro. Keratocytes were used to assess cytotoxicity on SF-CS (SFCS) blends, which was determined by a Cell Counting Kit-8 assay. The corneal lamellar scaffolds were developed with sequential culture techniques to form cell-scaffold constructs. Implantation was tested in 15 New Zealand White rabbits. The corneal substitutes were analysed by light and electron microscopy. Results: The reconstructed lamellar cornea was comparable to native tissue, with high levels of K3/12 expression in the corneal epithelial cells and vimentin in the stromal cells; moreover, the morphology and the position of the cells could be distinguished by histological methods. There was no sign of any immune reaction in or around the transplanted discs 12 weeks after implantation. Conclusion: A SFCS scaffold might be a suitable blend for corneal tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Use of a Silk Fibroin-Chitosan Scaffold to Construct a Tissue-Engineered Corneal Stroma

Guan

Tang

et al. 2013

Cells Tissues Organs

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“…In fact, powdered silkworm larvae were tested and shown in modern bioassays to inhibit absorption of glucose in human intestinal epithelium cells [81]. More recently, due to its unique properties, silk fibroin, a naturally occurring protein polymer, was shown to be a favorable matrix for the incorporation and delivery of a range of therapeutic agents [82]. Besides silk, other insect proteins are also used in medicine.…”

Section: Insect Products For Direct Use or Processingmentioning

confidence: 99%

Ecosystem Services Provided by the Little Things That Run the World

Ameixa‍¹,

Soares²,

Soares³

et al. 2018

Selected Studies in Biodiversity

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Highest extinction risk and consequently biodiversity loss are predicted to occur in invertebrates, specifically insects, and these declines are expected to cascade onto ecosystem functioning and human well-being. Although this knowledge is intrinsically present in more traditional communities, in more urban environments, mapping ecosystem services can be an important tool to raise people's awareness on the importance of preserving insect diversity. After an extensive revision of the available literature, we used a rule-based approach to assess the provisioning, regulating and maintenance, and cultural services delivered by insects. We followed the Common International Classification of Ecosystem Services (CICES) and identified several potential indicators that may help underpin the mapping and valuation of the services delivered by insects. From our search, we extracted a total of 73 indicators, divided as 17 Provisional indicators, 27 Regulation and Maintenance indicators, and 29 Cultural indicators. We concluded that insects are providers of services in the three major 'Sections' of ecosystem services defined by CICES. Despite the lack of recognition of provisioning and cultural services, the indicators provided may help to raise awareness on the importance of the little things the run the world, in order to preserve traditional and technological uses of insects and their services.

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“…However, a noticeable initial burst release of DoX was observed in water (8 %), pro 1 (0.5 %) and pro 10 (15 %) in the first three days, indicative of the enhanced dissolution of the active drugs within the core of the spheres. This effect is normally evident at 37 °C [24,39] and is expected to reduce by using higher SF concentrations for the starting solution [43]. After the first three days, the drug released slowly in a controlled manner within 2 weeks.The cumulative drug release from the spheres was fitted using a semi-empirical power law equation:…”

Section: Drug Release Kineticsmentioning

confidence: 99%

Doxorubicin loaded nanodiamond-silk spheres for fluorescence tracking and controlled drug release

Khalid

Mitropoulos

Marelli

et al. 2015

Biomed. Opt. Express

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Nanoparticle (NP) based technologies have proved to be considerably beneficial for advances in biomedicine especially in the areas of disease detection, drug delivery and bioimaging. Over the last few decades, NPs have garnered interest for their exemplary impacts on the detection, treatment, and prevention of cancer. The full potential of these technologies are yet to be employed for clinical use. The ongoing research and development in this field demands single multifunctional composite materials that can be employed simultaneously for drug delivery and biomedical imaging. In this manuscript, a unique combination of silk fibroin (SF) and nanodiamonds (NDs) in the form of nanospheres are fabricated and investigated. The spheres were loaded with the anthracyline Doxorubicin (DoX) and the drug release kinetics for these ND-SF-DoX (NDSX) spheres were studied. NDs provided the fluorescence modality for imaging while the degradable SF spheres stabilized and released the drug in a controlled manner. The emission and structural properties of the spheres were characterized during drug release. The degradability of SF and the subsequent release of DoX from the spheres were monitored through fluorescence of NDs inside the spheres. This research demonstrates the enormous potential of the ND-SF nanocomposite platforms for diagnostic and therapeutic purposes, which are both important for pharmaceutical research and clinical settings. "Nanodiamond-mediated drug delivery and imaging: challenges and opportunities," Expert Opin. Drug Deliv. 12(5), 735-749 (2015).

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Silk fibroin as a vehicle for drug delivery applications

Cited by 468 publications

References 150 publications

Use of a Silk Fibroin-Chitosan Scaffold to Construct a Tissue-Engineered Corneal Stroma

Use of a Silk Fibroin-Chitosan Scaffold to Construct a Tissue-Engineered Corneal Stroma

Ecosystem Services Provided by the Little Things That Run the World

Doxorubicin loaded nanodiamond-silk spheres for fluorescence tracking and controlled drug release

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