Silk Fibroin as a Functional Biomaterial for Drug and Gene Delivery

Tomeh, Mhd Anas; Hadianamrei, Roja; Zhao, Xiubo

doi:10.3390/pharmaceutics11100494

Cited by 141 publications

(96 citation statements)

References 133 publications

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“…Silk fibroin presents a combination of beneficial properties for drug delivery, including excellent biocompatibility [7], degradation to non-toxic products in vivo [8]. It provides easily accessible functional groups for chemical modifications [9].…”

Section: Introductionmentioning

confidence: 99%

Diclofenac Embedded in Silk Fibroin Fibers as a Drug Delivery System

Šišková

Kozma²,

Opálek

et al. 2020

Materials

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Silk fibroin is a biocompatible, non-toxic, mechanically robust protein, and it is commonly used and studied as a material for biomedical applications. Silk fibroin also gained particular interest as a drug carrier vehicle, and numerous silk formats have been investigated for this purpose. Herein, we have prepared electrospun nanofibers from pure silk fibroin and blended silk fibroin/casein, followed by the incorporation of an anti-inflammatory drug, diclofenac. Casein serves as an excipient in pharmaceutical products and has a positive effect on the gradual release of drugs. The characteristics of the investigated composites were estimated by scanning electron microscope, transmission electron microscope, thermogravimetric analysis, and a lifetime of diclofenac by electron paramagnetic resonance analysis. The cumulative release in vitro of diclofenac sodium salt, together with the antiproliferative effect of diclofenac sodium salt-loaded silk nanofibers against the growth of two cancer cell lines, are presented and discussed.

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Section: Introductionmentioning

confidence: 99%

Diclofenac Embedded in Silk Fibroin Fibers as a Drug Delivery System

Šišková

Kozma²,

Opálek

et al. 2020

Materials

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“…Silk fibroin particles have been utilized as promising drug delivery materials due to their biocompatibility, degradability, and ability to control drug release kinetics [ 31 ]. The silk fibroin particles prepared by salting out method and high-voltage electrospray method exhibited stable β -sheet structure, which stabilized the structure of the particles in the aqueous medium system (DMEM and PBS) [ 16 , 45 ].…”

Section: Discussionmentioning

confidence: 99%

“…Silk fibroin from Bombyx mori , is a versatile material for drug delivery systems because of its unique combination of biocompatibility, controllable biodegradability, mechanical stability, and diverse processing windows [ 30 ]. The active amino groups of silk fibroin provide opportunities for chemical modification [ 31 ], and it is feasible to label this protein with quantum dots. As a drug carrier, silk fibroin can be processed as films, gels, nanoparticles, microspheres, and 3D-scaffolds [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

The Interactions of Quantum Dot-Labeled Silk Fibroin Micro/Nanoparticles with Cells

et al. 2020

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When silk fibroin particles are used for controlled drug delivery, particle size plays a key role in the location of the carrier on the cells as well as the transport pathway, utilization efficiency, and therapeutic effect of the drugs. In this study, the interactions of different-sized silk fibroin particles and cell lines were investigated. Silk fibroin microparticles with dry size of 1.9 ± 0.4 μm (2.7 ± 0.3 μm in wet state) and silk fibroin nanoparticles with dry size of 51.5 ± 11.0 nm (174.8 ± 12.5 nm in wet state) were prepared by salting-out method and high-voltage electrospray method, respectively. CdSe/ZnS quantum dots were coupled to the surface of the micro/nanoparticles. Photostability observations indicated that the fluorescence stability of the quantum dots was much higher than that of fluorescein isothiocyanate. In vitro, microparticles and nanoparticles were co-cultured with human umbilical vein endothelial cells EA.hy 926 and cervical cancer cells HeLa, respectively. The fluorescence test and cell viability showed that the EA.hy926 cells tended to be adhered to the microparticle surfaces and the cell proliferation was significantly promoted, while the nanoparticles were more likely to be internalized in HeLa cells and the cell proliferation was notably inhibited. Our findings might provide useful information concerning effective drug delivery that microparticles may be preferred if the drugs need to be delivered to normal cell surface, while nanoparticles may be preferred if the drugs need to be transmitted in tumor cells.

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“…In addition, the degradation rate of SF also depends on its pore size, porosity, molecular weight distribution, and implantation sites. [102,103] Therefore, some effective strategies have been developed to obtain the desired degradation rate of SF, including adjusting the content of -sheet and changing the molecular weight distribution of SF.…”

Section: Propertiesmentioning

confidence: 99%

Silk‐Based Biomaterials for Cardiac Tissue Engineering

Song

Wang

Yue

et al. 2020

Adv Healthcare Materials

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Cardiovascular diseases are one of the leading causes of death globally. Among various cardiovascular diseases, myocardial infarction is an important one. Compared with conventional treatments, cardiac tissue engineering provides an alternative to repair and regenerate the injured tissue. Among various types of materials used for tissue engineering applications, silk biomaterials have been increasingly utilized due to their biocompatibility, biological functions, and many favorable physical/chemical properties. Silk biomaterials are often used alone or in combination with other materials in the forms of patches or hydrogels, and serve as promising delivery systems for bioactive compounds in tissue engineering repair scenarios. This review focuses primarily on the promising characteristics of silk biomaterials and their recent advances in cardiac tissue engineering.

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Silk Fibroin as a Functional Biomaterial for Drug and Gene Delivery

Cited by 141 publications

References 133 publications

Diclofenac Embedded in Silk Fibroin Fibers as a Drug Delivery System

Diclofenac Embedded in Silk Fibroin Fibers as a Drug Delivery System

The Interactions of Quantum Dot-Labeled Silk Fibroin Micro/Nanoparticles with Cells

Silk‐Based Biomaterials for Cardiac Tissue Engineering

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