Silk-Based Biomaterials in Cutaneous Wound Healing: A Systematic Review

Kamalathevan, Pragash; Ooi, Peng S; Loo, Yew Loong

doi:10.1097/01.asw.0000546233.35130.a9

Cited by 45 publications

(34 citation statements)

References 56 publications

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“…Additionally, SF has been developed into several forms of dressings, including sponges, films, scaffolds, electrospun nanofibers, and hydrogels [ 13 , 14 ]. Silk fibroin exists in alpha-helix and beta-sheet form, both of which are highly biocompatible and can also promote tissue regeneration [ 13 , 15 ]. However, it is difficult for amorphous silk fibroin (α-helix or random coil) to form stable scaffolds [ 16 , 17 ].…”

Section: Introductionsmentioning

confidence: 99%

Cutaneous Regeneration Mechanism of β-Sheet Silk Fibroin in a Rat Burn Wound Healing Model

et al. 2021

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Therapeutic dressings to enhance burn wound repair and regeneration are required. Silk fibroin (SF), a natural protein, induces cell migration and serves as a biomaterial in various dressings. SF dressings usually contain α-helices and β-sheets. The former has been confirmed to improve cell proliferation and migration, but the wound healing effect and related mechanisms of β-sheet SF remain unclear. We investigated the effects of β-sheet SF in vivo and in vitro. Alcohol-treated α-helix SF transformed into the β-sheet form, which promoted granulation formation and re-epithelialization when applied as lyophilized SF dressing (LSFD) in a rat burn model. Our in vitro results showed that β-sheet SF increased human dermal fibroblast (HDF) migration and promoted the expression of extracellular matrix (ECM) proteins (fibronectin and type III collagen), matrix metalloproteinase-12, and the cell adhesion molecule, integrin β1, in rat granulation tissue and HDFs. This confirms the role of crosstalk between integrin β1 and ECM proteins in cell migration. In summary, we demonstrated that β-sheet SF facilitates tissue regeneration by modulating cell adhesion molecules in dermal fibroblasts. LSFD could find clinical application for burn wound regeneration. Moreover, β-sheet SF could be combined with anti-inflammatory materials, growth factors, or antibiotics to develop novel dressings.

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Section: Introductionsmentioning

confidence: 99%

Cutaneous Regeneration Mechanism of β-Sheet Silk Fibroin in a Rat Burn Wound Healing Model

et al. 2021

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“…In both in vitro and in vivo studies on wound healing, SF-based biomaterials have demonstrated good cell adhesion and fibroblast proliferation, with improved neovascularization [ 42 ], faster and better tissue healing and complete regeneration in a rat model [ 43 ].…”

Section: Silk Fibroin As a Nature Derived Materials For Wound Healimentioning

confidence: 99%

Bioinspired Materials for Wound Healing Application: The Potential of Silk Fibroin

Pollini

Paladini

2020

Materials

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Nature is an incredible source of inspiration for scientific research due to the multiple examples of sophisticated structures and architectures which have evolved for billions of years in different environments. Numerous biomaterials have evolved toward high level functions and performances, which can be exploited for designing novel biomedical devices. Naturally derived biopolymers, in particular, offer a wide range of chances to design appropriate substrates for tissue regeneration and wound healing applications. Wound management still represents a challenging field which requires continuous efforts in scientific research for definition of novel approaches to facilitate and promote wound healing and tissue regeneration, particularly where the conventional therapies fail. Moreover, big concerns associated to the risk of wound infections and antibiotic resistance have stimulated the scientific research toward the definition of products with simultaneous regenerative and antimicrobial properties. Among the bioinspired materials for wound healing, this review focuses attention on a protein derived from the silkworm cocoon, namely silk fibroin, which is characterized by incredible biological features and wound healing capability. As demonstrated by the increasing number of publications, today fibroin has received great attention for providing valuable options for fabrication of biomedical devices and products for tissue engineering. In combination with antimicrobial agents, particularly with silver nanoparticles, fibroin also allows the development of products with improved wound healing and antibacterial properties. This review aims at providing the reader with a comprehensive analysis of the most recent findings on silk fibroin, presenting studies and results demonstrating its effective role in wound healing and its great potential for wound healing applications.

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“…Recent studies have shown that SS is biocompatible and has good biological performance. SS shows great hydrophilic and has multiple biological properties, including anti-oxidation, anti-bacterial, and promoting cell adhesion and proliferation ( 20 , 21 ). However, SS shows poor mechanical properties.…”

Section: Introductionmentioning

confidence: 99%

Human Umbilical Cord Mesenchymal Stem Cell Derived Exosomes Delivered Using Silk Fibroin and Sericin Composite Hydrogel Promote Wound Healing

Han

Liu

Chen

et al. 2021

Front. Cardiovasc. Med.

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Recent studies have shown that the hydrogels formed by composite biomaterials are better choice than hydrogels formed by single biomaterial for tissue repair. We explored the feasibility of the composite hydrogel formed by silk fibroin (SF) and silk sericin (SS) in tissue repair for the excellent mechanical properties of SF, and cell adhesion and biocompatible properties of SS. In our study, the SF SS hydrogel was formed by SF and SS protein with separate extraction method (LiBr dissolution for SF and hot alkaline water dissolution for SS), while SF-SS hydrogel was formed by SF and SS protein using simultaneous extraction method (LiBr dissolution for SF and SS protein). The effects of the two composite hydrogels on the release of inflammatory cytokines from macrophages and the wound were analyzed. Moreover, two hydrogels were used to encapsulate and deliver human umbilical cord mesenchymal stem cell derived exosomes (UMSC-Exo). Both SF SS and SF-SS hydrogels promoted wound healing, angiogenesis, and reduced inflammation and TNF-α secretion by macrophages. These beneficial effects were more significant in the experimental group treated by UMSC-Exo encapsulated in SF-SS hydrogel. Our study found that SF-SS hydrogel could be used as an excellent alternative to deliver exosomes for tissue repair.

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Silk-Based Biomaterials in Cutaneous Wound Healing: A Systematic Review

Cited by 45 publications

References 56 publications

Cutaneous Regeneration Mechanism of β-Sheet Silk Fibroin in a Rat Burn Wound Healing Model

Cutaneous Regeneration Mechanism of β-Sheet Silk Fibroin in a Rat Burn Wound Healing Model

Bioinspired Materials for Wound Healing Application: The Potential of Silk Fibroin

Human Umbilical Cord Mesenchymal Stem Cell Derived Exosomes Delivered Using Silk Fibroin and Sericin Composite Hydrogel Promote Wound Healing

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