Siliconizing glassware to be used for suspension cell culture

Noteboom, William D.; Will, Peter C.

doi:10.1007/bf01666873

Cited by 4 publications

(2 citation statements)

References 4 publications

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“…Hibara et al demonstrated how combining structuring together with a modified surface allowed for a counter-current flow between nitrobenzene and H 2 O inside a glass microchip using an octadecylsilane coating [14]. Depositing self-assembled monolayers of silanes with low surface energy tail groups is a common method to avoid stiction effects in MEMS devices [15,16], and glass surfaces are readily made hydrophobic with silanes to reduce sticking of valuable reagents in biological applications [17].…”

Section: Page 3 Of 30mentioning

confidence: 99%

Influence of surface modifications and channel structure for microflows of supercritical carbon dioxide and water

Knaust

Andersson

Hjort

et al. 2016

The Journal of Supercritical Fluids

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Section: Page 3 Of 30mentioning

confidence: 99%

Influence of surface modifications and channel structure for microflows of supercritical carbon dioxide and water

Knaust

Andersson

Hjort

et al. 2016

The Journal of Supercritical Fluids

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“…All glassware used in this study was first siliconized by applying dichlorodimethylsilane (Sigma-Aldrich, Shanghai, China) with ≥99.5% concentration on all surfaces that came in contact with microcarrier beads [23]. After siliconization, the glasswares were then sterilized by autoclaving.…”

Section: Siliconization Of Glasswarementioning

confidence: 99%

Propagation and Molecular Characterization of Fowl Adenovirus Serotype 8b Isolates in Chicken Embryo Liver Cells Adapted on Cytodex™ 1 Microcarrier Using Stirred Tank Bioreactor

et al. 2020

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Large volume production of vaccine virus is essential for prevention and control of viral diseases. The objectives of this study were to propagate Fowl adenovirus (FAdV) isolate (UPM08136) in chicken embryo liver (CEL) cells adapted to Cytodex™ 1 microcarriers using stirred tank bioreactor (STB) and molecularly characterize the virus. CEL cells were prepared and seeded onto prepared Cytodex™ 1 microcarriers and incubated first in stationary phase for 3 h and in STB at 37 °C, 5% CO2, and 20 rpm for 24 h. The CEL cells were infected with FAdV isolate (UPM08136) passage 5 (UPM08136CELP5) or passage 20 (UPM08136CELP20) and monitored until cell detachment. Immunofluorescence, TCID50, sequencing, alignment of hexon and fiber genes, and phylogenetic analysis were carried out. CEL cells were adapted well to Cytodex™ 1 microcarriers and successfully propagated the FAdV isolates in STB with virus titer of 107.5 (UPM08136CELP5B1) and 106.5 (UPM08136CELP20B1) TCID50/mL. These isolates clustered with the reference FAdV serotype 8b in the same evolutionary clade. The molecular characteristics remained unchanged, except for a point substitution at position 4 of the hexon gene of UPM08136CELP20B1, suggesting that propagation of the FAdV isolate in STB is stable and suitable for large volume production and could be a breakthrough in the scale-up process.

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