Peritoneal macrophages are distinct from monocytes and adherent macrophages

Selvarajan, Krithika; Moldovan, Leni; Chandrakala, Aluganti N.; Litvinov, Dmitry; Parthasarathy, S.

doi:10.1016/j.atherosclerosis.2011.09.014

Cited by 20 publications

(17 citation statements)

References 30 publications

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“…However, these data are consistent with the relative expression of Abca1 and Abcg1 in naive peritoneal macrophages that are noninfl ammatory ( 62,63 ) isolated from obese db/db mice for which Abcg1 was highly expressed ( 17 ).…”

Section: Discussionsupporting

confidence: 81%

ABCG1 regulates mouse adipose tissue macrophage cholesterol levels and ratio of M1 to M2 cells in obesity and caloric restriction

Tarling

McMillen

et al. 2015

Journal of Lipid Research

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Supplemental Material can be found at: 2338 Journal of Lipid Research Volume 56, 2015ATMs and that Abcg1 plays a key role in modulating ATM cholesterol levels in obesity and acute weight loss.ATMs were collected from mice for which the myeloid cells had been replaced by bone marrow transplantation (BMT) from Abcg1 Ϫ / Ϫ mice and from WT mice undergoing autologous BMT. We found that Abcg1 expression affected the steady-state cholesterol levels of ATMs, with Abcg1ATMs having the highest total cholesterol content. Other sterol species were not evaluated due to limitations in ATM cell quantities. We found that the ratio of M1 to M2 ATMs was altered with loss of Abcg1 expression, and that Abcg1 was most highly expressed by M2 rather than proinfl ammatory M1 macrophages suggesting that M2 cells have a unique role in adipose tissue sterol homeostasis. Overall, this study identifi es ABCG1 as an important protein determining the relative levels of ATMs and cholesterol metabolism especially in M2 macrophages. Understanding the contribution of ABCG1 to ATM cholesterol homeostasis may provide new information for controlling obesity, weight loss, and related disorders such as lung, liver, and gallbladder diseases ( 38-40 ). MATERIALS AND METHODS MiceIsogenic WT C57BL/6 mice were purchased from the Jackson Laboratory (Bar Harbor, ME; #000664). ABCG1 knockout ( Abcg1 Ϫ / Ϫ ) mice on a C57BL/6 background were generated as previously described ( 41 ). BMT was conducted using C57BL/6 male mice as acceptors of either C57BL/6 bone marrow cells (WT-BMT) or cells from Abcg1 Ϫ / Ϫ mice ( Abcg1 Ϫ / Ϫ BMT) using procedures as described below. Femurs taken from Abcg1Ϫ / Ϫ mice were placed in PBS and sent overnight on ice from the University of California, Los Angeles to the University of Washington where bone marrow cells were isolated. In addition, C57BL/6 male mice raised at the University of Washington (three to four generations from the Jackson Laboratory) were used as a reference strain for measures of body composition. All mice were housed in pathogen-free conditions, in a temperature-and humidity-controlled environment (12 h light/dark cycle). Animal procedures were reviewed and approved by the Institutional Animal Care and Use Committee of University of Washington. Study designBMT was performed essentially as described ( 42 ). Briefl y, male C57BL/6 mice (WT) at 13 weeks of age were irradiated (950 rads) and then engrafted with either WT or Abcg1 Ϫ / Ϫ bone marrow cells (5 × 10 6 cells/mouse; n = 20 in each group) by retroorbital injection. Mice were maintained on pelleted rodent chow (LabDiet 5053; Purina Mills, St. Louis, MO) and acidifi ed water with neomycin (X-gen Pharmaceuticals, Big Flats, NY) for 4 weeks of recovery. Mice were then fed a high-fat diet (HFD) [D12492; Research Diets Inc., New Brunswick, NJ; 60% kcal (primarily lard) and 20% kcal carbohydrate (maltodextrin 10 and sucrose)] for 13 weeks. Mice were then randomly assigned to one of two groups for another 3 weeks of either continued ad libitum HFD feeding or to...

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Section: Discussionsupporting

confidence: 81%

ABCG1 regulates mouse adipose tissue macrophage cholesterol levels and ratio of M1 to M2 cells in obesity and caloric restriction

Tarling

McMillen

et al. 2015

Journal of Lipid Research

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“…Monocytes were purifi ed from the rest of monocytic cells by 1 h plastic adherence followed by extensive washing with 1× PBS (pH 7.4) to remove nonadherent cells ( 27 ). Macrophages from the peritoneal cavity were isolated by peritoneal lavage using 3 ml of cold saline, centrifuged, and plated for 1 h in serum-free RPMI-1640 at 37°C in a 5% CO 2 incubator followed by washes with 1× PBS to remove nonadherent cells ( 28 ).…”

Section: Supplementary Abstract Atherosclerosis • Dyslipidemias • Fomentioning

confidence: 99%

Differential lipid metabolism in monocytes and macrophages: influence of cholesterol loading

Fernández-Ruíz

Puchalska

Narasimhulu

et al. 2016

Journal of Lipid Research

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“…Peritoneal macrophages (pMΦs) were harvested from pre-stimulated C57BL/6 mice (female, 6-8 weeks), according to a previously described protocol [19]. To detect antigen uptake, different vaccine formulations (Alexa Fluor 488-labelled OVA, 5 μg/ mL; microparticles, 100 μg/mL) were incubated with pMΦs in 24-well plates.…”

Section: Preparation and Characterization Of Plga Microparticles And mentioning

confidence: 99%

Immunopotentiator-Loaded Polymeric Microparticles as Robust Adjuvant to Improve Vaccine Efficacy

et al. 2015

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Peritoneal macrophages are distinct from monocytes and adherent macrophages

Cited by 20 publications

References 30 publications

ABCG1 regulates mouse adipose tissue macrophage cholesterol levels and ratio of M1 to M2 cells in obesity and caloric restriction

ABCG1 regulates mouse adipose tissue macrophage cholesterol levels and ratio of M1 to M2 cells in obesity and caloric restriction

Differential lipid metabolism in monocytes and macrophages: influence of cholesterol loading

Immunopotentiator-Loaded Polymeric Microparticles as Robust Adjuvant to Improve Vaccine Efficacy

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