Silicon Nanomaterials for Biosensing and Bioimaging Analysis

Ji, Xiaoyuan; Wang, Houyu; Song, Bin; Chu, Binbin; He, Yao

doi:10.3389/fchem.2018.00038

Cited by 91 publications

(44 citation statements)

References 63 publications

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“…The developed immobilization platform enables to cover a broad spectrum of industrially and medically relevant materials. Six materials were selected: Gold is used for applications in medicine and biotechnology (for instance in biosensors) due to optical, thermal, and electronic properties (Homaei et al, ); PET is utilized, for instance, in blood contacting devices due to its nonimmunogenicity (Ramachandran, Chakraborty, Kannan, Dixit, & Muthuvijayan, ); stainless steel is commonly used in orthopedic areas and bears the advantage of corrosion resistance combined with biocompatibility (Mohd Daud, Hussein al‐Ashwal, Abdul Kadir, & Saidin, ); PS and PP are synthetic polymers utilized for instance as enzyme carriers (Jesionowski et al, ); silicon‐based materials are, for instance, most frequently used semiconductor material in microelectronics and nanoelectronics and find application in, for example, biosensing (Estephan et al, ; Ji, Wang, Song, Chu, & He, ).…”

Section: Introductionmentioning

confidence: 99%

Matter‐tag: A universal immobilization platform for enzymes on polymers, metals, and silicon‐based materials

Dedisch

Wiens

Davari

et al. 2019

Biotech & Bioengineering

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Enzyme immobilization is extensively studied to improve enzyme properties in catalysis and analytical applications. Here, we introduce a simple and versatile enzyme immobilization platform based on adhesion‐promoting peptides, namely Matter‐tags. Matter‐tags immobilize enzymes in an oriented way as a dense monolayer. The immobilization platform was established with three adhesion‐promoting peptides; Cecropin A (CecA), liquid chromatography peak I (LCI), and Tachystatin A2 (TA2), that were genetically fused to enhanced green fluorescent protein and to two industrially important enzymes: a phytase (from Yersinia mollaretii) and a cellulase (CelA2 from a metagenomic library). Here, we report a universal and simple Matter‐tag–based immobilization platform for enzymes on various materials including polymers (polystyrene, polypropylene, and polyethylene terephthalate), metals (stainless steel and gold), and silicon‐based materials (silicon wafer). The Matter‐tag–based enzyme immobilization is performed at ambient temperature within minutes (<10 min) in an aqueous solution harboring the phytase or cellulase by immersing the targeted material. The peptide LCI was identified as universal adhesion promoter; LCI immobilized both enzymes on all investigated materials. The attachment of phytase‐LCI onto gold was characterized with surface plasmon resonance spectroscopy obtaining a dissociation constant value (KD) of 2.9·10−8 M and a maximal surface coverage of 504 ng/cm².

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Section: Introductionmentioning

confidence: 99%

Matter‐tag: A universal immobilization platform for enzymes on polymers, metals, and silicon‐based materials

Dedisch

Wiens

Davari

et al. 2019

Biotech & Bioengineering

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“…Esto gracias a las propiedades que presentan las NPSis como un alta y estable intensidad de fluorescencia [58]. En virtud de estas mismas características, es posible realizar caracterizaciones sistemáticas del bio-comportamiento de las NPSis en los mecanismos de internalización celular, tráfico intracelular y su metabolización, elementos esenciales en la evaluación toxicológica de estos nanomateriales [59][60][61][62].…”

Section: Imagenología Biológicaunclassified

“…En laúltima década, se han alcanzado logros emocionantes en elárea de los sensores basados en nanomateriales de silicio y nanohíbridos, los cuales han permitido a su vez determinar una gama amplia de especies químicas y biológicas en formas importantemente confiables y sensibles [58]. En esta sección se hará enfasis en los sensores basados en silicio, como lo son el transistor de efecto de campo (FET, siglas en inglés), sensores fluorescentes y los sensores por dispersión Raman en superficie mejorada (SERS, siglas en inglés).…”

Section: Biosensoresunclassified

“…Tomando ventaja de estas características, los sustratos o matrices basados en NESis híbridas han permitido el análisis de diversas muestras químicas y biológicas en función de buena sensibilidad y confiabilidad, por ejemplo, en células apoptóticas [79], bacterias [80], iones plomo [81]. Gracias a estas importantes aplicaciones, se han diseñados SERS portátiles con excelentes plataformas analíticas, que pueden convertirse también en herramientas valiosas para el análisis ambiental en campo [58]. Recientes investigaciones [82] han determinado nuevas fuentes de silicio de origen natural.…”

Section: Biosensoresunclassified

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Nanoestructuras De Silicio en Biomedicina Y Biotecnología

Rios¹,

Vega-Baudrit

Villegas³

et al. 2020

Momento

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Silicon nanostructures have properties that make them appealing for applications in biomedicine and biotechnology. The fabrication and characterization of silicon nanostructures are currently gaining a lot of attention and they can include nano-wires, whiskers, pillars, tubes, cones, particles, etc. There are several methods to assist the generation of these nanostructures like laser ablation, thermal evaporation decomposition, and chemical vapor deposition, among others. We consider they are highly potential tools for the improving of diagnosis techniques and the treatment of important biological conditions. The objective of this review is to summarize the main aspects of the physicochemical design of silicon nanomaterials, in addition to toxicological considerations regarding biocompatibility, biodistribution and cell dynamics. Finally, we emphasis on important biomedical applications, such as gene therapy, drug delivery, imaging and sensoring.

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“…Nanocrystalline silicon (nc-Si) has been studied extensively for the last few years due to the possibility to use it for the production of optical [1], light emitting [2] and electronic [3,4] devices and biosensors [5]. Silicon dioxide that is formed on the surface of silicon nanocrystals is of great importance for some other applications such as fabrication of integrated circuits.…”

Section: Introductionmentioning

confidence: 99%

Oxidation of nanocrystalline silicon at room temperature and various humidity

Popelensky¹,

Dorofeev²,

Kononov³

et al. 2019

Preprint

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Oxidation of HF vapor-etched nanocrystalline silicon films, prepared by drop coating from nanocrystalline Si sol in acetonitrile, was studied. Oxidation of nc-Si at room temperature in air with 5% and 86% relative humidity was observed by means of IR spectroscopy for 2 days. The change in film mass after 15 hours of oxidation was determined using quartz crystal microbalance. In dry air, film mass and integral intensity of bands attributed to vibrations in Si3-x‒Si‒Hx and Si-O-Si groups changed linearly with time. In humid air, intensity of in Si3-x‒Si‒Hx band decays exponentially and intensity of Si-O-Si band increases as a square root of oxidation time. Film mass gain after 15 hours of oxidation corresponds to an average oxide layer thickness of 0.02 nm in dry air and 0.51 nm in wet air.

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Silicon Nanomaterials for Biosensing and Bioimaging Analysis

Cited by 91 publications

References 63 publications

Matter‐tag: A universal immobilization platform for enzymes on polymers, metals, and silicon‐based materials

Matter‐tag: A universal immobilization platform for enzymes on polymers, metals, and silicon‐based materials

Nanoestructuras De Silicio en Biomedicina Y Biotecnología

Oxidation of nanocrystalline silicon at room temperature and various humidity

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