Silicon-mediated enhancement of physiological and biochemical characteristics of Zinnia elegans ‘Dreamland Yellow’ grown under salinity stress

Manivannan, Abinaya; Soundararajan, Prabhakaran; Arum, Laras Sekar; Ko, Chung Ho; Muneer, Sowbiya; Jeong, Byoung Ryong

doi:10.1007/s13580-015-1081-2

Cited by 26 publications

(16 citation statements)

References 48 publications

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“…CAT activity was assessed by the change in absorbance at 240 nm during incubation of the extracts (100 mL) at 25°C with 10 mM H 2 O 2 in a 50 mM phosphate buffer (pH 7.0) in a total volume of 3 mL (Wang et al, 2004b). POD activity was detected at 460 nm with 18 mM guaiacol and 5 mM H 2 O 2 in a 50 mM sodium acetate buffer (pH 5.4) in a total volume of 3 mL of enzyme extract (Manivannan et al, 2015a). APX activity was measured in a reaction mixture containing a 50 mM potassium phosphate buffer (pH 7.0), 5 mM ascorbic acid, 0.1 mM H 2 O 2 , and 100 mL enzyme extract.…”

Section: Methodsmentioning

confidence: 99%

Effects of Rare Earth Elements on Growth and Determination of Secondary Metabolites under In Vitro Conditions in Salvia miltiorrhiza

Fan¹,

Zhang²,

Wang³

et al. 2020

horts

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Salvia miltiorrhiza, known as danshen, is one of most valued medicinal plants in China. Although it has been cultivated since ancient times, an optimal culture system needs to be standardized for this important species. Here, we explored the phytochemical properties of S. miltiorrhiza with the treatments of rare earth elements (REEs) to develop an optimal tissue culture system. Four-week-old in vitro-grown S. miltiorrhiza plantlets were used as explants. The experiment was conducted in a randomized block design on a Murashige and Skoog (MS) medium containing 0.2 mg·L−1 naphthaleneacetic acid (NAA) to induce rooting at four different concentrations (50, 100, 200, and 300 μM) of REEs such as cerium (Ce), lanthanum (La), or praseodymium (Pr), respectively. Compared with all REEs at different concentrations, 100 μM Pr induced greater root length than Ce or La at any concentrations. Concomitantly, 0.38 μg tanshinone IIA/mg dry weight (DW) was observed, which was 54.84% higher than in the control. Similarly, chlorophyll content, antioxidant enzyme activity, and secondary metabolite were enhanced in rooting medium supplemented with 100 μM Pr. Therefore, this study showed that 100 μM Pr is an adequate concentration in the optimal culture system for promoting plant growth as well as enhancing secondary metabolite content in S. miltiorrhiza.

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Section: Methodsmentioning

confidence: 99%

Effects of Rare Earth Elements on Growth and Determination of Secondary Metabolites under In Vitro Conditions in Salvia miltiorrhiza

Fan¹,

Zhang²,

Wang³

et al. 2020

horts

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“…Several studies reported that Si modulated the redox-homeostasis mechanism. Supplemented Si regulated the activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), guaiacolperoxidase (GPX), and ascorbate peroxidase (APX) to overcome the redox imbalance [17,18,19]. In addition, lower molecular non-enzymatic antioxidants such as glutathione and proline content under the stress condition was improved by the Si supplementation [20].…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of Silicon-Mediated Amelioration of Salt Stress in Plants

Liu

Soundararajan

Manivannan

2019

Plants

102

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Silicon (Si), the second most predominant element in the earth crust consists of numerous benefits to plant. Beneficial effect of Si has been apparently visible under both abiotic and biotic stress conditions in plants. Supplementation of Si improved physiology and yield on several important agricultural and horticultural crops. Salinity is one of the major abiotic stresses that affect growth and yield. The presence of high concentration of salt in growing medium causes oxidative, osmotic, and ionic stresses to plants. In extreme conditions salinity affects soil, ground water, and limits agricultural production. Si ameliorates salt stress in several plants. The Si mediated stress mitigation involves various regulatory mechanisms such as photosynthesis, detoxification of harmful reactive oxygen species using antioxidant and non-antioxidants, and proper nutrient management. In the present review, Si mediated alleviation of salinity stress in plants through the regulation of photosynthesis, root developmental changes, redox homeostasis equilibrium, and regulation of nutrients have been dealt in detail.

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“…The absorbance of the supernatant was measure at 530 nm and the superoxide content was estimated using the standard sodium nitrite calibration curve. The endogenous H 2 O 2 level was measured at 390 nm using the standard H 2 O 2 calibration curve [ 40 ].…”

Section: Methodsmentioning

confidence: 99%

“…Then the homogenate was centrifuged at 10,000× g for 20 min at 4 °C and the supernatant was used for determination of antioxidant enzymes activity. Superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and guaiacol peroxidase (GPX) enzyme activities were estimated by following the protocols of Manivannan et al [ 40 ]. The total protein content was estimated at 595 nm according to Bradford method [ 41 ] using bovine serum albumin as standard.…”

Section: Methodsmentioning

confidence: 99%

Chemical Elicitor-Induced Modulation of Antioxidant Metabolism and Enhancement of Secondary Metabolite Accumulation in Cell Suspension Cultures of Scrophularia kakudensis Franch

Manivannan

Soundararajan

Park

et al. 2016

IJMS

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Scrophularia kakudensis is an important medicinal plant with pharmaceutically valuable secondary metabolites. To develop a sustainable source of naturaceuticals with vital therapeutic importance, a cell suspension culture was established in S. kakudensis for the first time. Friable calli were induced from the leaf explants cultured on a Murashige and Skoog (MS) medium containing 3.0 mg¨L´1 6-benzyladenine (BA) in a combination with 2 mg¨L´1 2,4-dichlorophenoxy acetic acid (2,4-D). From the callus cultures, a cell suspension culture was initiated and the cellular differentiation was investigated. In addition, the effect of biotic elicitors such as methyl jasmonate (MeJa), salicylic acid (SA), and sodium nitroprusside (SNP) on the accumulation of secondary metabolites and antioxidant properties was demonstrated. Among the elicitors, the MeJa elicited the accumulation of total phenols, flavonoids, and acacetin, a flavonoid compound with multiple pharmaceutical values. Similarly, the higher concentrations of the MeJa significantly modulated the activities of antioxidant enzymes and enhanced the scavenging potentials of free radicals of cell suspension extracts. Overall, the outcomes of this study can be utilized for the large scale production of pharmaceutically important secondary metabolites from S. kakudensis through cell suspension cultures.

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Silicon-mediated enhancement of physiological and biochemical characteristics of Zinnia elegans ‘Dreamland Yellow’ grown under salinity stress

Cited by 26 publications

References 48 publications

Effects of Rare Earth Elements on Growth and Determination of Secondary Metabolites under In Vitro Conditions in Salvia miltiorrhiza

Effects of Rare Earth Elements on Growth and Determination of Secondary Metabolites under In Vitro Conditions in Salvia miltiorrhiza

Mechanisms of Silicon-Mediated Amelioration of Salt Stress in Plants

Chemical Elicitor-Induced Modulation of Antioxidant Metabolism and Enhancement of Secondary Metabolite Accumulation in Cell Suspension Cultures of Scrophularia kakudensis Franch

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