Silicon dioxide surfaces with aryl interaction sites for chromatographic applications

At the current state of knowledge, the rational optimization of the chromatographic separation of peptides, as well as the identification of proteins in proteomics are challenges for analytical chemists. In this paper the generalized applicability of a recently derived descriptor log Sum(k+1)AA in a QSRR equation to model peptide retention in RP-LC systems was evaluated. For that purpose, two sets of peptides analyzed on dissimilar RP-LC systems were considered. A first set of 28 peptides was measured on 17 columns/systems, while a second of 70 peptides was eluted on four. The aim of this work was to confirm the usefulness of the partly experimental log Sum(k+1)AA descriptor for the prediction of peptides retention compared to the initially applied, fully experimental log SumAA descriptor. The verification of the predictive abilities of both QSRR models, applying either the initial or the alternative descriptor, was done by using the leave-one-out and leave-three-out cross-validation procedures. The results seem to demonstrate that the QSRR model with log Sum(k+1)AA, for which the retention measurement of only seven out of 20 existing amino acids is necessary, possesses similar or in some cases even better predictive abilities than that containing log SumAA.

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“…( The reaction mechanism and conditions for the synthesis of the home-made cholesterolic and phenyl phases were described earlier [33,34].…”

Section: Equipment and Chromatographic Conditionsmentioning

confidence: 99%

Evaluation of a generalized use of the log Sum(k+1)_AA descriptor in a QSRR model to predict peptide retention on RPLC systems

Bodzioch¹,

Dejaegher²,

Bączek³

et al. 2009

J of Separation Science

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“…However, remains the most popular separation and determination technique high performance liquid chromatography (HPLC) (53)(54)(55)(56)(57). Recently, optimization of determination conditions for some ESsilanates by HPLC methods and capillary electrophoresis (CE) (58-61) was reported.…”

Section: Preparation Of Samples To Analysismentioning

confidence: 99%

Analytical Techniques in Determination of Biologically Active Organosilicons of the ES-Silanate Group

Kluska¹

2008

Critical Reviews in Analytical Chemistry

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An attempt of synthesis of information concerning chosen organosilicon compounds of the group of electrostatically stabilized (ES)-silanates is described. All hypercoordinated organosilicon derivatives show biological activity. This activity is very important for the whole ecosystem. Some of these compounds easily hydrolyze; it causes various difficulties during their isolation from different matrices and while preparing the samples to chromatographic analysis. Hypercoordinated ES-silanates can be determinate by the use of high performance liquid chromatography (HPLC) as well as capillary electrophoresis.

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“…Baker, Łódź, Poland) and naphthylpropyl (RP Si-NAF, Figure 2). [12] Dimensions of the steel columns were: for RP Si-C 18 -250 Â 4.6 mm, for RP Si-C 8 and RP Si-NAF -125 Â 4.6 mm (Table 3). Two anhydrous systems of mobile phase were applied: acetonitrile and dichloromethane.…”

Section: Experimental Hplc Analysis Of (E)-azastilbenesmentioning

confidence: 99%

Separation of Biologically Active Isomers of (E)‐N‐Meta‐ and Para‐Nitroazastilbenes by the HPLC Technique

Prukała

Pypowski²,

Chrząścik³

et al. 2008

Journal of Liquid Chromatography & Related Technologies

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Results of investigations concerning the chromatography of bromides of (E)-N-mand p-nitrobenzyl-4 0 -hydroxy-3 0 -methoxystilbazoles-4 are presented. Analyzed compounds, similarly to other azastilbene halogenides show antimicrobial activity. Optimal conditions of chromatographic separation and determination of two mentioned isomers have been elaborated. In the investigation three stationary phases (octadecyl, octyl, and naphthylpropyl), two mobile phases (acetonitrile, dichloromethane) and various flow were considered. The best selectivity and the highest separation factor, a ¼ 1.74, were obtained using a naphthylpropyl column and 100% acetonitrile, as the mobile phase. An application of an octadecyl phase, recommended by numerous analysts as standard, allowed only the observation of an existence of two compounds, but did not yield satisfactory results.

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Silicon dioxide surfaces with aryl interaction sites for chromatographic applications

Cited by 43 publications

References 43 publications

Evaluation of a generalized use of the log Sum(k+1)_AA descriptor in a QSRR model to predict peptide retention on RPLC systems

Evaluation of a generalized use of the log Sum(k+1)_AA descriptor in a QSRR model to predict peptide retention on RPLC systems

Analytical Techniques in Determination of Biologically Active Organosilicons of the ES-Silanate Group

Separation of Biologically Active Isomers of (E)‐N‐Meta‐ and Para‐Nitroazastilbenes by the HPLC Technique

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Silicon dioxide surfaces with aryl interaction sites for chromatographic applications

Cited by 43 publications

References 43 publications

Evaluation of a generalized use of the log Sum(k+1)AA descriptor in a QSRR model to predict peptide retention on RPLC systems

Evaluation of a generalized use of the log Sum(k+1)AA descriptor in a QSRR model to predict peptide retention on RPLC systems

Analytical Techniques in Determination of Biologically Active Organosilicons of the ES-Silanate Group

Separation of Biologically Active Isomers of (E)‐N‐Meta‐ and Para‐Nitroazastilbenes by the HPLC Technique

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Product

Resources

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Evaluation of a generalized use of the log Sum(k+1)_AA descriptor in a QSRR model to predict peptide retention on RPLC systems

Evaluation of a generalized use of the log Sum(k+1)_AA descriptor in a QSRR model to predict peptide retention on RPLC systems