Silicate-based bioceramics regulating osteoblast differentiation through a BMP2 signalling pathway

Zhai, Dong; Xu, Mengchi; Liu, Liqi; Chang, Jiang; Wu, Chengtie

doi:10.1039/c7tb01931a

Cited by 38 publications

(19 citation statements)

References 38 publications

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“…Therefore, our ALP secretion profile is consistent and in agreement with the cellular adhesion, ECM protein adsorption, and proliferation profiles, as indicated above. In addition, Zhai et al proved that the Si ion released from a Ca–Si–Sr bioceramic significantly affects the OC gene and protein expression that not only activates Smad1/5-BMP2 signaling pathways, but also promotes osteogenic gene transcription in conjunction with the transcription factor Runx2 [18].…”

Section: Resultsmentioning

confidence: 99%

“…A recent study by Chen et al reported that Mg–CS has better degradation rates than neat CS, thus making Mg–CS more suitable for clinical applications [17]. In addition, CS was found to be hydrophilic in nature, and SBF immersed CS was reported to exhibit continuous release of Ca and Si ions throughout the entire immersion period [18,19]. These factors were found to enhance initial cellular adhesion, which led to subsequent downstream up-regulation of cell attachment, proliferation, differentiation, extracellular matrix secretion, and bone tissue formation [20].…”

Section: Introductionmentioning

confidence: 99%

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Improved Bioactivity of 3D Printed Porous Titanium Alloy Scaffold with Chitosan/Magnesium-Calcium Silicate Composite for Orthopaedic Applications

et al. 2019

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Recently, cases of bone defects have been increasing incrementally. Thus, repair or replacement of bone defects is gradually becoming a huge problem for orthopaedic surgeons. Three-dimensional (3D) scaffolds have since emerged as a potential candidate for bone replacement, of which titanium (Ti) alloys are one of the most promising candidates among the metal alloys due to their low cytotoxicity and mechanical properties. However, bioactivity remains a problem for metal alloys, which can be enhanced using simple immersion techniques to coat bioactive compounds onto the surface of Ti–6Al–4V scaffolds. In our study, we fabricated magnesium-calcium silicate (Mg–CS) and chitosan (CH) compounds onto Ti–6Al–4V scaffolds. Characterization of these surface-modified scaffolds involved an assessment of physicochemical properties as well as mechanical testing. Adhesion, proliferation, and growth of human Wharton’s Jelly mesenchymal stem cells (WJMSCs) were assessed in vitro. In addition, the cell attachment morphology was examined using scanning electron microscopy to assess adhesion qualities. Osteogenic and mineralization assays were conducted to assess osteogenic expression. In conclusion, the Mg–CS/CH coated Ti–6Al–4V scaffolds were able to exhibit and retain pore sizes and their original morphologies and architectures, which significantly affected subsequent hard tissue regeneration. In addition, the surface was shown to be hydrophilic after modification and showed mechanical strength comparable to natural bone. Not only were our modified scaffolds able to match the mechanical properties of natural bone, it was also found that such modifications enhanced cellular behavior such as adhesion, proliferation, and differentiation, which led to enhanced osteogenesis and mineralization downstream. In vivo results indicated that Mg–CS/CH coated Ti–6Al–4V enhances the bone regeneration and ingrowth at the critical size bone defects of rabbits. These results indicated that the proposed Mg–CS/CH coated Ti–6Al–4V scaffolds exhibited a favorable, inducive micro-environment that could serve as a promising modification for future bone tissue engineering scaffolds.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Improved Bioactivity of 3D Printed Porous Titanium Alloy Scaffold with Chitosan/Magnesium-Calcium Silicate Composite for Orthopaedic Applications

et al. 2019

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“…However, the underlying mechanism of SrCS and its role in enhancing cellular activities remains unknown. Local extracellular ionic changes were previously reported to have certain enhancing effects on cellular activities [35]. In this study, there was an initial burst release of Si ions from the SrCS scaffold after day 1, and eventually decreased to a gradual release for the rest of the immersion period.…”

Section: Discussionmentioning

confidence: 49%

The Calcium Channel Affect Osteogenic Differentiation of Mesenchymal Stem Cells on Strontium-Substituted Calcium Silicate/Poly-ε-Caprolactone Scaffold

Tp¹,

Huang

Kao

et al. 2020

Processes

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There had been a paradigm shift in tissue engineering studies over the past decades. Of which, part of the hype in such studies was based on exploring for novel biomaterials to enhance regeneration. Strontium ions have been reported by others to have a unique effect on osteogenesis. Both in vitro and in vivo studies had demonstrated that strontium ions were able to promote osteoblast growth, and yet at the same time, inhibit the formation of osteoclasts. Strontium is thus considered an important biomaterial in the field of bone tissue engineering. In this study, we developed a Strontium-calcium silicate scaffold using 3D printing technology and evaluated for its cellular proliferation capabilities by assessing for protein quantification and mineralization of Wharton’s Jelly mesenchymal stem cells. In addition, verapamil (an L-type of calcium channel blocker, CCB) was used to determine the mechanism of action of strontium ions. The results found that the relative cell proliferation rate on the scaffold was increased between 20% to 60% within 7 days of culture, while the CCB group only had up to approximately 10% proliferation as compared with the control specimen. Besides, the CCB group had downregulation and down expressions of all downstream cell signaling proteins (ERK and P38) and osteogenic-related protein (Col I, OPN, and OC). Furthermore, CCB was found to have 3–4 times lesser calcium deposition and quantification after 7 and 14 days of culture. These results effectively show that the 3D printed strontium-contained scaffold could effectively stimulate stem cells to undergo bone differentiation via activation of L-type calcium channels. Such results showed that strontium-calcium silicate scaffolds have high development potential for bone tissue engineering.

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“…Several studies indicated the ideal bioscaffold must be able to release various ions in a sustained manner in order to accomplish and maintain the therapeutic effect, and yet, at the same time, this release profile should be controllable to avert harmful effects [43]. In the previous study, the extracellular ionic variation was regulated the cellular behaviors such as migration, proliferation, and differentiation [44]. The detailed amount of ions quantity nor release rates in various solutions such as SBF, PBS and culture medium at which it will stimulate in-vivo osteogenesis is not yet definitively determined, though such values derived from several studies for Si (0.02 to 50 ppm) and Sr (8.7 to 87.6 ppm) had been previously studied and discussed [45].…”

Section: Immersion Behaviormentioning

confidence: 99%

Enhanced Proliferation and Differentiation of Human Mesenchymal Stem Cell-laden Recycled Fish Gelatin/Strontium Substitution Calcium Silicate 3D Scaffolds

Wang

Liu

et al. 2020

Applied Sciences

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Cell-encapsulated bioscaffold is a promising and novel method to allow fabrication of live functional organs for tissue engineering and regenerative medicine. However, traditional fabrication methods of 3D scaffolds and cell-laden hydrogels still face many difficulties and challenges. This study uses a newer 3D fabrication technique and the concept of recycling of an unutilized resource to fabricate a novel scaffold for bone tissue engineering. In this study, fish-extracted gelatin was incorporated with bioactive ceramic for bone tissue engineering, and with this we successfully fabricated a novel fish gelatin methacrylate (FG) polymer hydrogel mixed with strontium-doped calcium silicate powder (FGSr) 3D scaffold via photo-crosslinking. Our results indicated that the tensile strength of FGSr was almost 2.5-fold higher as compared to FG thus making it a better candidate for future clinical applications. The in-vitro assays illustrated that the FGSr scaffolds showed good biocompatibility with human Wharton jelly-derived mesenchymal stem cells (WJMSC), as well as enhancing the osteogenesis differentiation of WJMSC. The WJMSC-laden FGSr 3D scaffolds expressed a higher degree of alkaline phosphatase activity than those on cell-laden FG 3D scaffolds and this result was further proven with the subsequent calcium deposition results. Therefore, these results showed that 3D-printed cell-laden FGSr scaffolds had enhanced mechanical property and osteogenic-related behavior that made for a more suitable candidate for future clinical applications. Furthermore, bone regeneration is a complex physiological process regulated by osteoclast resorption with osteoblast bone formation and the entire process includes inflammatory reactions, endochondral bone formation and bone remodeling. Currently, for clinical cases, the golden method to repair bone defects is to use autologous bone grafts as this method avoids disease spreading and immunological rejection. However, autografts have some major drawbacks such as limited availability and invasive harvesting that requires additional surgery to harvest healthy bones from another site. Furthermore, additional surgeries mean increased surgical risks and complications [2]. Allograft is an alternative to bone repair; however, it is highly limited due to rejection and lack of availability. To solve this problem, researchers had attempted to develop novel biomaterials including ceramics, polymers and composites as an alternative choice for bone regeneration [3][4][5]. In order to promote bone healing, the material must be biocompatible, biodegradable and able to promote bone regeneration which mimics the distinctive property of natural bone [6]. With the emergence of newer 3D printing techniques, we are now able to fabricate scaffolds with customized shape, pore sizes and architectures that are similar to native bone [7,8].For many years, bioceramics has emerged as promising biomaterial for bone regeneration [9,10]. Calcium phosphate is one of the most popular bioceramics which had been repeate...

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Silicate-based bioceramics regulating osteoblast differentiation through a BMP2 signalling pathway

Abstract: Si-containing bioactive ionic products released from silicate-based bioceramics activate Smad1/5-mediated BMP2 signaling pathways.

Cited by 38 publications

References 38 publications

Improved Bioactivity of 3D Printed Porous Titanium Alloy Scaffold with Chitosan/Magnesium-Calcium Silicate Composite for Orthopaedic Applications

Improved Bioactivity of 3D Printed Porous Titanium Alloy Scaffold with Chitosan/Magnesium-Calcium Silicate Composite for Orthopaedic Applications

The Calcium Channel Affect Osteogenic Differentiation of Mesenchymal Stem Cells on Strontium-Substituted Calcium Silicate/Poly-ε-Caprolactone Scaffold

Enhanced Proliferation and Differentiation of Human Mesenchymal Stem Cell-laden Recycled Fish Gelatin/Strontium Substitution Calcium Silicate 3D Scaffolds

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