Silica gels activated by BCl₃ and aliphatic diamines as supports for glucoamylase immobilization

Cinnamic ester of D‐sorbitol for immobilization of mushroom tyrosinase

“…Precipitation of the cinnamoyl derivative (ie the immobilization support) on glass beads (ie the inert matrix) was carried out by dissolvent evaporation 32. The best precipitation results were obtained at support concentrations close to 15 g dm −3 29.…”

Stability of horseradish peroxidase immobilized on different cinnamic carbohydrate esters

“…This means that this support would be very stable at the normal work temperatures used in those industrial applications where it is suitable, even without cross‐linking. The derivatives were cross‐linked by irradiation in the ultraviolet region 15, 23…”

Immobilization of enzymes on porous silica supports

“…Slightly favoured pattern of porosity seems t o have silica 1 for which thesoniewhat higher stability is observed for all studied enzymes. Therefore we suppose that this type , of Si-gel porosity seems to be the optimal for biotechnological plant scale enzymes immobilization, taking into account also that the Si-gels activation were performed using easier and less expensive activat,ion technique with SiC1, instead of BCI, [6].…”

“…A number of enzymes have been succesfully bound to silica; for instance, alkylaniinated silica supports were found to be an excellent support for hydrogenase [5] and for glucoamylase used in the pilot plant production of glucose [2]. It was also successfully used by us in the immobilization of glucoamylase [6]. The positive results with this support encouraged us to check the influence of silica structure on protein immobilization.…”