Stability of horseradish peroxidase immobilized on different cinnamic carbohydrate esters

Abstract

ABTS m). However, the affinity of HRP immobilized on the INCN derivative for these substrates was higher than that shown by the enzyme in solution. In turn, the enzyme immobilized on all the supports was more resistant than the soluble form to inactivation by H 2 O 2 and by heat at neutral pH. When the stability and durability of the immobilized HRP were analysed, the cinnamoylated derivatives prepared were seen to function as suitable supports for immobilizing peroxidase and to be suitable for industrial application of the enzyme.

Studies on the activity and stability of immobilized horseradish peroxidase on poly(ethylene terephthalate) grafted acrylamide fiber

“…Support material, which plays an important role in the utility of an immobilized enzyme, should be readily available and non-toxic and also should provide a large surface area suitable for enzyme reaction, and substrate and product transport with least-diffusional restriction [8]. Some organic and inorganic supports like glass beads [9], polyketone [10], chitosan [11] and aluminum oxide/polyethyleneimine composite [12] have been used for the immobilization of peroxidases.…”

Enzyme Technology of Peroxidases: Immobilization, Chemical and Genetic Modification

“…The immobilized HRP was more resistant to inactivation by urea, Triton X-100, and organic solvents compared to its soluble counterpart [41]. [51,52] (Fig. 9.6) were assayed for noncovalently immobilized HRP.…”

“…All the supports were more resistant than the soluble form to inactivation by H 2 O 2 and by heat at neutral pH. When the stability and durability of the immobilized HRP were analyzed, the cinnamoylated derivatives functioned as suitable supports for immobilizing peroxidase and to be suitable for industrial application of the enzyme [51,52]. Sol-gel encapsulation of biological catalysts in silicate glasses has experienced a swift expansion due to the synthetic ease of enzyme entrapment procedures and to the significantly enhanced stability of the entrapped enzyme [101][102][103].…”

“…The thermostability of the immobilized HRP was greater than that of the free enzyme and also showed great [51,52] (continued) Chemically modified HRP by using anhydrides of mono-and dicarboxilic acids and pycril sulfonic acid…”

A comparative study of free and immobilized soybean and horseradish peroxidases for 4-chlorophenol removal: protective effects of immobilization

“…Many supports and methods have also been used for HRP immobilization: magnetite by physical adsorption [11], glass beads by different methods of covalent coupling [12][13][14], a wide variety of polymers in many diverse configurations [15][16][17][18][19], and ion exchange resins [20]. Some of these immobilized derivatives have been applied to 4-chlorophenol removal with promising results [11,14,15].…”