Silica‐Coated CaF₂:Eu³⁺ Nanoparticles Functionalized with Oxalic Acid for Bio‐conjugation to BSA Proteins

Abstract

A new method for silica-coated CaF 2 :Eu 3 ＋ core-shell nanoparticles functionalized with oxalic acid for bio-conjugation to bovine serum albumin (BSA) proteins has been developed. Moreover, CaF 2 :Eu 3＋ /SiO 2 core-shell nanoparticles modified with oxalic acid are biocompatible and can be dispersed in water. As an organic functional molecule, oxalic acid is able to react with hydroxyl groups existed on the surface of SiO 2 layer by esterification reaction to form carboxylic acid for further bio-conjugation with BSA. The final products were characterized by means of X-ray diffraction (XRD), transmission electron microscope (TEM), field-emission scanning electron microscopy (FE-SEM), ultraviolet (UV) spectrophotometer, infrared (IR) spectrophotometer and photoluminescence (PL) spectra. XRD result confirmed the phase purity of with pH ranging from 2 to 9. Therefore, these luminescent colloidal nanoparticles can be potentially employed as targeted fluorescent labels in biomedical research applications.

Bright X-ray and up-conversion nanophosphors annealed using encapsulated sintering agents for bioimaging applications

“…41, 42 However, such multicomponent materials can be complex to characterize, especially in biological systems and CaF 2 can be difficult to functionalize without additional shells. 43 Thus, the synthesis of brighter X-ray and up-conversion nanophosphors remains a significant challenge.…”

Glucose detection using SERS with multi-branched gold nanostructures in aqueous medium

“…Moreover, the silanization of various metal nanoparticle systems has shown great success in protecting their surface characteristics and facilitating bioconjugation. 52,53 The SERS activity of gold and silver nanostructures has been experimentally veried using rhodamine B as an analyte due to its distinct Raman features and adsorbability onto nanoparticles. 54,55 One of the important applications of SERS is detection of glucose.…”

Fluorescent Silica Nanoparticles for Medical Imaging

“…2,[27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45] Other smaller molecules (proteins, antibodies, cofactors) require specialized conjugation to the SiNP. 38,[46][47][48][49][50][51][52][53][54][55][56][57][58][59][60][61] Even the addition of magnetic NP conjugation to the FSNP 3,4,62 greatly assists in the recognition and tracking of different organic molecules.…”