Silica Aerogel Improves the Biocompatibility in a Poly-<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:mi mathvariant="bold">ε</mml:mi></mml:mrow></mml:math>-Caprolactone Composite Used as a Tissue Engineering Scaffold

This study is to investigate the effect of synthesis approaches on morphology, porosity, and biocompatibility of bioactive glass (BG). BG prepared through sol–gel approach was subsequently subjected to microwave and probe sonication techniques to investigate the structural and morphological effect. Hexagonal rod‐shaped morphology was obtained in sol–gel‐derived bioactive glass, whereas mesoporous particles and spherical‐shaped morphology were observed in probe‐sonicated and microwave‐assisted sol–gel approaches, respectively. The probe‐sonicated BG has mesopores with pore diameter of 14.7 nm, whereas surface porosity of 1.5 nm, and 3.5 nm for pure sol–gel and microwave‐assisted sol–gel fabricated BGs. Granular size, shape, and porosity have a significant role at the point of contact with cellular membrane. Therefore, we studied the biocompatibility with respect to morphology and porosity of the fabricated BGs. From this study, we observed that the BG prepared using probe sonication method controls the particle size, further it enhances the porosity that altogether improves the biocompatibility. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 108B:143–155, 2020.

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“…Cell viability % was calculated using the following formula (Eq. 2)

Cell viability % = \frac{Sample absorbance}{Control} \times 100

…”

Section: Methodsmentioning

confidence: 99%

Effect of microwave and probe sonication processes on sol–gel‐derived bioactive glass and its structural and biocompatible investigations

2019

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“…In this context, a well-diversified collection of biopolymers has been considered for the preparation of bio-aerogels, including collagen [ 10 ], gelatin [ 11 ], whey proteins [ 12 ], etc. Also polysaccharides such as alginate [ 13 ], cellulose [ 14 ], chitosan (CS) [ 15 ] and many others have been successfully used to produce bio-based aerogels [ 16 , 17 ]. Among the natural compounds, CS (a biopolymer extracted from chitin natural source) has attracted great interest due to their remarkable properties for industrial technology and biomedical fields [ 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Chitosan-GPTMS-Silica Hybrid Mesoporous Aerogels for Bone Tissue Engineering

et al. 2020

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This study introduces a new synthesis route for obtaining homogeneous chitosan (CS)-silica hybrid aerogels with CS contents up to 10 wt%, using 3-glycidoxypropyl trimethoxysilane (GPTMS) as coupling agent, for tissue engineering applications. Aerogels were obtained using the sol-gel process followed by CO2 supercritical drying, resulting in samples with bulk densities ranging from 0.17 g/cm3 to 0.38 g/cm3. The textural analysis by N2-physisorption revealed an interconnected mesopore network with decreasing specific surface areas (1230–700 m2/g) and pore sizes (11.1–8.7 nm) by increasing GPTMS content (2–4 molar ratio GPTMS:CS monomer). In addition, samples exhibited extremely fast swelling by spontaneous capillary imbibition in PBS solution, presenting swelling capacities from 1.75 to 3.75. The formation of a covalent crosslinked hybrid structure was suggested by FTIR and confirmed by an increase of four hundred fold or more in the compressive strength up to 96 MPa. Instead, samples synthesized without GPTMS fractured at only 0.10–0.26 MPa, revealing a week structure consisted in interpenetrated polymer networks. The aerogels presented bioactivity in simulated body fluid (SBF), as confirmed by the in vitro formation of hydroxyapatite (HAp) layer with crystal size of approximately 2 µm size in diameter. In vitro studies revealed also non cytotoxic effect on HOB® osteoblasts and also a mechanosensitive response. Additionally, control cells grown on glass developed scarce or no stress fibers, while cells grown on hybrid samples showed a significant (p < 0.05) increase in well-developed stress fibers and mature focal adhesion complexes.

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“…Its biocompatible property has been also proved for tissue engineering scaffold application of poly-3-caprolactone composite. 40 Aerogel minimize the acidic condition aer polymer decomposition. Therefore 3T3 and primary mouse osteoblastic cells were long term stabilized around pH level of 7.2 and 7.4.…”

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confidence: 99%

Calf thymus DNA characterization and its adsorption on different silica surfaces

Yetgin

Balköse

2015

RSC Adv.

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DNA adsorption is the initial stage of gene therapy for drug delivery systems and solid phase extraction methods of DNA purification. High pore volume and high adsorption capacity are simple requirements not only for producing 'smart' drug delivery systems but also the development of purification kits. Silica is the most used material for this purpose. The present study aimed at elucidating the calf thymus DNA biosorption process by the characterization of calf thymus DNA and silica to increase the efficiency of the currently used silica material. Mesoporous silica has long been used for DNA adsorption and silica aerogel is the new adsorbent investigated in the present study. When DNA solution was freeze dried on a silica wafer, self-assembled super helices formed as shown by atomic microscopy (AFM). Thus DNA existed not as single molecules but as large sized agglomerates in water. Thus it could be adsorbed in the macropores and on the external surface of adsorbents. Adsorption of calf thymus DNA to a silica aerogel, a mesoporous silica gel and a silica wafer was investigated in the present study. Silica aerogel was synthesized from TEOS by a supercritical ethanol drying process. The DNA adsorption capacity of the silica aerogel was nearly two times that of the mesoporous silica gel due to its macroporous structure and its higher silanol content. Silica aerogel was found to be a very promising material for DNA adsorption. Therefore silica aerogel can be considered as a good candidate for the delivery of DNA.

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Silica Aerogel Improves the Biocompatibility in a Poly-ε-Caprolactone Composite Used as a Tissue Engineering Scaffold

Cited by 27 publications

References 35 publications

Effect of microwave and probe sonication processes on sol–gel‐derived bioactive glass and its structural and biocompatible investigations

Effect of microwave and probe sonication processes on sol–gel‐derived bioactive glass and its structural and biocompatible investigations

Chitosan-GPTMS-Silica Hybrid Mesoporous Aerogels for Bone Tissue Engineering

Calf thymus DNA characterization and its adsorption on different silica surfaces

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