Silencing the nosocomial pathogen Serratia marcescens by glyceryl trinitrate

Abbas, Hisham A.; Elsherbini, Ahmed

doi:10.4314/ahs.v18i1.2

Cited by 7 publications

(12 citation statements)

References 29 publications

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“…Moreover, using FDA approved drug saves the high costs and shortens the long development times to produce new antimicrobials or virulence inhibitors33. Our previous studies showed that GTN is a potent inhibitor of quorum sensing and virulence in Pseudomonas aeruginosa and Serratia marcescens 34,35. The rationale of repurposing drugs for use as anti-virulence agents against Stapylococcus aureus was previously reported.…”

Section: Discussionmentioning

confidence: 99%

Glyceryl trinitrate blocks staphyloxanthin and biofilm formation in Staphylococcus aureus

Abbas¹,

Elsherbini²,

Shaldam³

2019

Afr H. Sci.

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Background Staphylococcus aureus is an important nosocomial bacterium that is responsible for a number of infections that may be fatal. The treatment of such infections is limited by emergence of antibiotic resistance. Targeting virulence of Staphylococcus aureus may provide an alternative option to antibiotic that may bypass the emergence of resistant strains due to lack of stress on viability. Objectives Investigation of the ability of glyceryl trinitrate (GTN) to inhibit staphyloxanthin, biofilm and tolerance to oxidative stress. Methods The disk sensitivity method was used to detect the methicillin resistance of Staphylococcus aureus . The effect of sub-inhibitory concentration of GTN on biofilm formation, staphyloxanthin production and tolerance to oxidative stress was evaluated. Molecular docking study was used to investigate the ability of GTN to bind to dehydrosqualene synthase enzyme. Results GTN showed a significant inhibition of biofilm, staphyloxanthin and tolerance to oxidative stress. In the molecular docking study, it was found that GTN could bind to dehydrosqualene synthase enzyme by hydrogen bonding, electrostatic interaction and pi-cation interaction. Conclusion The present study revealed the ability of GTN to serve as a potential anti-virulence candidate for attenuation of S. aureus pathogenicity.

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Section: Discussionmentioning

confidence: 99%

Glyceryl trinitrate blocks staphyloxanthin and biofilm formation in Staphylococcus aureus

Abbas¹,

Elsherbini²,

Shaldam³

2019

Afr H. Sci.

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“…For example, Abbas and coworkers [59] showed that diclofenac sodium was able to inhibit the virulence of S. aureus. In addition, it was reported that GTN reduced several virulence factors in S. aureus, Pseudomonas aeruginosa and Serratia marcescens [32,75,76]. To our knowledge, our study is the first study that investigate the EPI activity of diclofenac, domperidone and GTN.…”

Section: Plos Onementioning

confidence: 75%

Promising FDA-approved drugs with efflux pump inhibitory activities against clinical isolates of Staphylococcus aureus

et al. 2022

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Background and objectives Staphylococcus aureus is an opportunistic pathogen that causes wide range of nosocomial and community-acquired infections which have spread worldwide leading to an urgent need for developing effective anti-staphylococcal agents. Efflux is an important resistance mechanism that bacteria used to fight the antimicrobial action. This study aimed to investigate the efflux mechanism in S. aureus and assess diclofenac, domperidone, glyceryl trinitrate and metformin as potential efflux pump inhibitors that can be used in combination with antibiotics for treating topical infections caused by S. aureus. Materials and methods Efflux was detected qualitatively by the ethidium bromide Cart-Wheel method followed by investigating the presence of efflux genes by polymerase chain reaction. Twenty-six isolates were selected for further investigation of efflux by Cart-Wheel method in absence and presence of tested compounds followed by quantitative efflux assay. Furthermore, antibiotics minimum inhibitory concentrations in absence and presence of tested compounds were determined. The effects of tested drugs on expression levels of efflux genes norA, fexA and tetK were determined by quantitative real time-polymerase chain reaction. Results Efflux was found in 65.3% of isolates, the prevalence of norA, tetK, fexA and msrA genes were 91.7%, 77.8%, 27.8% and 6.9%. Efflux assay revealed that tested drugs had potential efflux inhibitory activities, reduced the antibiotic’s MICs and significantly decreased the relative expression of efflux genes. Conclusion Diclofenac sodium, domperidone and glyceryl trinitrate showed higher efflux inhibitory activities than verapamil and metformin. To our knowledge, this is the first report that shows that diclofenac sodium, glyceryl trinitrate and domperidone have efflux pump inhibitory activities against S. aureus.

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“…Nevertheless, South African Grade 8 learners are engaging in risky sexual behaviour and require sexual and reproduction education programmes tailored to the age group 21 . Adolescents require sexual negotiation sklls, improved sexual decision making and the ability to critically examine social scripts and peer pressures 10 , 16 .…”

Section: Discussionmentioning

confidence: 99%

“…Various interventions, such as HIV counselling and testing (HCT) and the LoveLife education programme have been proposed at global, national and school level but they have been ineffective due to stigmatisation and the prospect of a positive HIV outcome (Department of Health and Department of Basic Education, 2012). Many adolescents, who remain untested, are not receiving counselling and are engaging in risky behaviour that cause transmission of HIV 10 . Gender power imbalances (associated with significantly older partners in particular), early sexual debut, barriers to contraceptive use, misinformation on sexual health matters and lack of parental support at home also increase the risk of teenage pregnancies 11 .…”

Section: Introductionmentioning

confidence: 99%

Sexual knowledge and practice of adolescent learners in a rural South African school

et al. 2020

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Background: Premature sexual activity has become a norm in South African society, often resulting in teenage pregnancy and sexually transmitted diseases (STD). Occurrence of premature sexual activity is related to insufficient education, gender inequal- ities, household poverty and place of residence. The Stepping Stones project uses a 10-session programme to educate learners about relationships, HIV-prevention and teenage pregnancy. The purpose was to measure and describe learners’ sexual knowl- edge and activities in a rural technical secondary school in North-west Province, South Africa. Methods: A cross-sectional survey. Questionnaires were distributed to learners in grade 8 to 12. Descriptive statistics was used in analysis. Results: Seventy-nine questionnaires were analysed. Despite a young sample, 26.6% were sexually active and 24.1% engaged in sexual activity. The mean age for first-time sexual intercourse was 15.2±2.3 years. The use of contraceptives was low (41.2%) and participants reported difficulty in talking to partners about condom use (54.8%). Almost half (45.5%) of the participants had never heard of STDs. Participants expressed a need to use social media as a sex education tool (12.3%). The primary source of information was from school-based programmes (58.0%). Conclusion: Findings point to unsafe sexual practice of learners at a school in rural South Africa, even from an early age. This concern is accompanied by the occurrence of low levels of sexually-related knowledge. The learners would benefit from contin- ued implementation of the Stepping Stones programme. Implementation could be improved by incorporating social media and emphasising gender equality and negotiation skills in sexually vulnerable situations. Keywords: Sexual knowledge; adolescent learners; South Africa.

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Silencing the nosocomial pathogen Serratia marcescens by glyceryl trinitrate

Cited by 7 publications

References 29 publications

Glyceryl trinitrate blocks staphyloxanthin and biofilm formation in Staphylococcus aureus

Glyceryl trinitrate blocks staphyloxanthin and biofilm formation in Staphylococcus aureus

Promising FDA-approved drugs with efflux pump inhibitory activities against clinical isolates of Staphylococcus aureus

Sexual knowledge and practice of adolescent learners in a rural South African school

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