Silencing of synaptotagmin 13 inhibits tumor growth through suppressing proliferation and promoting apoptosis of colorectal cancer cells

Abstract

The treatment of colorectal cancer is currently hampered by the lack of early detection technology. The identification of molecular biomarkers for colorectal cancer is crucial for improving prognosis. Synaptotagmin (SYT) 13 has been reported to be associated with several human tumors, but its role in colorectal cancer remains elusive. In the present study, immunohistochemistry was utilized to detect the expression of SYT13 in colorectal cancer tissues and cells. MTT, colony formation, wound healing and Transwell assays were conducted to evaluate the effect of SYT13 knockdown on the biological behavior of RKO and HCT116 cells. Cell apoptosis and cell cycle profiles were detected by FACS. A mouse xenograft model was constructed to investigate the effect of SYT13 on colorectal cancer in vivo. The results indicated that SYT13 was upregulated in colorectal tumor tissues compared with paracancerous tissues. Silencing of SYT13 inhibited the proliferation, colony formation, migration and invasion ability of RKO and HCT116 cells. Moreover, SYT13 knockdown arrested the cell cycle in the G2 phase, thus inducing cell apoptosis. The in vivo experiments also demonstrated the inhibitory effect of SYT13 on tumor growth. In conclusion, the present study demonstrated that SYT13 may act as a promoter in the development and progression of colorectal cancer and, therefore, may be of value as a target for the development of novel treatment strategies.

A concurrent canonical and modified miRNAome pan-cancer study on TCGA and TARGET cohorts leads to an enhanced resolution in cancer

“…The copyright holder for this preprint this version posted May 20, 2021. ; https://doi.org/10.1101/2021.05.18.444694 doi: bioRxiv preprint SYT13 in breast cancer (TCGA-BRCA), an oncogenic gene in different cancers (116,117,(122)(123)(124)(125)(126)(127).…”

Comprehensive analysis on expression profile and prognostic values of SYTs family members and their methylation in gastric cancer

“…However, several studies have recently reported that SYTs family members had vital roles in the pathogenesis of human cancer. It was discovered that SYT13 was up-regulated in the lung adenocarcinoma as well as in colorectal cancer, which promoted the lung adenocarcinoma cells and colorectal cancer cells proliferation and migration, and contributed to unsatisfactory prognosis 10,11 . Sung HY et al demonstrated that SYT2 was upregulated in ovarian cancer, promoted the ovarian carcinoma cells migration and invasiveness, and associated with poor survival for patients with ovarian cancer 12 .…”

Prognosis and immune function of Synaptotagmin-4 in gastric cancer and brain low-grade glioma

“…These studies reflected the effects of the SYTs family on immune cells and the diversity of their functions. In the terms of the relationship between SYTs and tumors, a research found that SYT13 was helpful for the occurrence and development of colorectal cancer [10]. SYT7 was beneficial for the development of osteosarcoma [11].…”

“…There is still no research to explore the relationship between SYT4 and cancer, but some studies have found the relationship between other members of SYTs and cancer. Qin Li et al reported that SYT13 was helpful for the occurrence and development of colorectal cancer [10]. Wu Z et al reported that SYT7 was beneficial for the development of osteosarcoma [11].…”