Silencing of rice stripe virus in Laodelphax striatellus using virus-derived double-stranded RNAs

An, Saes Byeol; Choi, Jae Young; Lee, Seok Hee; Fāng, Yīng; Kim, Jong Hoon; Park, Dong Hwan; Park, Min Gu; Woo, Ra Mi; Kim, Woo Jin; Je, Yeon Ho

doi:10.1016/j.aspen.2017.04.009

Cited by 5 publications

(6 citation statements)

References 22 publications

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“…The RSV-infested rice used in this study was kindly provided by the National Institute of Crop Science, Miryang, Korea. RSV-viruliferous L. striatellus was obtained by feeding 2nd instar nymphs of naive L. striatellus on RSV-infected rice (5-6 cm tall) for 5 days, with RSV infection confirmed by reverse transcription polymerase chain reaction as previously described (An et al, 2017). The RSV-viruliferous L. striatellus individuals were provided RSV-infested rice periodically and maintained in the laboratory at 28°C and 80% relative humidity under a 16 h light/8 h dark photocycle.…”

Section: Methodsmentioning

confidence: 99%

“…RNAi-mediated control of plant viruses in their vector insects could be achieved either directly by silencing of viral transcripts or indirectly by silencing host genes essential for viral replication (Kanakala and Ghanim, 2016). Replication of RSV in L. striatellus has been efficiently suppressed not only by application of exogenous RSV-specific dsRNAs (An et al, 2017) but also by transcriptional silencing of L. striatellus genes, specifically, CRP1 and LsE75 (Fang et al, 2017;Liu et al, 2015). In a previous comparative transcriptomic analysis, we identified L. striatellus genes that are strongly upregulated in RSV-viruliferous L. striatellus (Lee et al, 2013).…”

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confidence: 99%

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Suppression of Rice Stripe Virus Replication in Laodelphax striatellus Using Vector Insect-Derived Double-Stranded RNAs

et al. 2020

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RNA interference (RNAi) has attracted attention as a promising approach to control plant viruses in their insect vectors. In the present study, to suppress replication of the rice stripe virus (RSV) in its vector, Laodelphax striatellus, using RNAi, dsRNAs against L. striatellus genes that are strongly upregulated upon RSV infection were delivered through a rice leaf-mediated method. RNAi-based silencing of peroxiredoxin, cathepsin B, and cytochrome P450 resulted in significant down regulation of the NS3 gene of RSV, achieving a transcriptional reduction greater than 73.6% at a concentration of 100 ng/µl and, possibly compromising viral replication. L. striatellus genes might play crucial roles in the transmission of RSV; transcriptional silencing of these genes could suppress viral replication in L. striatellus. These results suggest effective RNAi-based approaches for controlling RSV and provide insight into RSV-L. striatellus interactions.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

Suppression of Rice Stripe Virus Replication in Laodelphax striatellus Using Vector Insect-Derived Double-Stranded RNAs

et al. 2020

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“…L. striatellus ingested dsRNA using the rice‐mediated feeding RNAi method as described previously (An et al, 2017). Fifteen fourth instar nymphs of L. striatellus were infested over rice leaves steeped in 300 µl of each dsRNA solution (30, 60, 120, 180, and 250 ng/µl in 10% sucrose solution).…”

Section: Methodsmentioning

confidence: 99%

“…2.6 | Bioassays using dsRNA L. striatellus ingested dsRNA using the rice-mediated feeding RNAi method as described previously (An et al, 2017).…”

Section: Dsrna Synthesis and Quantificationmentioning

confidence: 99%

Insecticidal and synergistic activity of dsRNAs targeting buprofezin‐specific genes against the small brown planthopper, Laodelphax striatellus

Fāng

Park

Choi

et al. 2020

Arch Insect Biochem Physiol

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The small brown planthopper, Laodelphax striatellus, is a dangerous pest in rice fields. Although buprofezin has been used to control L. striatellus for more than a decade, the occurrence of buprofezin-resistant L. striatellus has been recently reported. To develop an alternative pest control strategy, comparative transcriptome analysis of buprofezin-treated and nontreated L. striatellus was performed to screen the buprofezin-specific target genes for RNA interference (RNAi) application. Among six genes downregulated in the buprofezin-treated L. striatellus, RNAi-based silencing of the lipophorin precursor, endocuticle structure glycoprotein, and chitin synthase significantly induced the lethality of L. striatellus in a concentration-dependent manner. In addition, a cocktail of double-stranded RNAs against these three genes showed synergistic effects with buprofezin. These results provide RNAi-based effective approaches to control L. striatellus as well as an efficient method to identify novel target genes for RNAi application.

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“…In addition, CP can not only protect the viral genome from proteolytic enzymes and nucleases but also transfer the viral genome through specific binding to host cell receptors. The knockdown of CP by RNA interference significantly inhibits the replication of RSV genomes [20]. Recent studies have shown that CP and viral genomic RNA were partially transported to the nucleus of the vector and affected the immune response by utilizing the nuclear importin protein α transport system [21,22].…”

Section: Introductionmentioning

confidence: 99%

Rice Stripe Virus Infection Facilitates the Reproductive Potential of Laodelphax striatellus

Zhang,

Yu,

et al. 2024

Agronomy

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The rice stripe virus (RSV) is transmitted by the small brown planthopper Laodelphax striatellus in a persistent and circulative–propagative manner. During the last few decades, RSV has caused serious disease outbreaks in rice fields in China. The results of long-term coevolution have led to complex and diverse relationships between viruses and vector insects, and understanding these interactions is critical for the management of diseases and vector organisms. In this study, three groups of comparative experiments were performed to investigate the effects of RSV infection on the reproductive parameters, vitellogenin (Vg), and Vg receptor (VgR) expressions of L. striatellus. The results showed that RSV infection promoted the fecundity, hatchability, and Vg and VgR expressions of L. striatellus but had no effect on the preoviposition period and oviposition period. Furthermore, the RNA interference of the nucleocapsid protein (CP) gene and the injection of the CP antibody in RSV-viruliferous L. striatellus reduced the fecundity, hatchability, and Vg and VgR expressions, further strengthening the hypothesis that RSV may manipulate the reproductive behavior of insect vectors to promote its own transmission. The results of this study can further reveal the interaction mechanism of virus–vector insects in reproduction and virus transmission, and provide new insights for the control of insect-borne viruses.

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Silencing of rice stripe virus in Laodelphax striatellus using virus-derived double-stranded RNAs

Cited by 5 publications

References 22 publications

Suppression of Rice Stripe Virus Replication in Laodelphax striatellus Using Vector Insect-Derived Double-Stranded RNAs

Suppression of Rice Stripe Virus Replication in Laodelphax striatellus Using Vector Insect-Derived Double-Stranded RNAs

Insecticidal and synergistic activity of dsRNAs targeting buprofezin‐specific genes against the small brown planthopper, Laodelphax striatellus

Rice Stripe Virus Infection Facilitates the Reproductive Potential of Laodelphax striatellus

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