Silencing of OBP genes: Generation of loss-of-function mutants of PBP by genome editing

Shiota, Yusuke; Sakurai, Takeshi

doi:10.1016/bs.mie.2020.05.009

Cited by 2 publications

(1 citation statement)

References 41 publications

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“…At this time, the antennae tips (approximately 1 mm) were cut with scissors to measure the potential difference. After excising the antennae and setting the electrodes, to fix the antennae and improve their connection with the electrodes, we applied a conductive gel (Spectra 360 Electrode Conductive Gel, Parker Laboratories Inc., USA), which is commonly employed for measuring EAGs, (19,20) using pointed tweezers. After installing the antennae, they were connected to the amplifiers and their responses to odor stimuli were measured.…”

Section: Design Of Printed Electrodementioning

confidence: 99%

Dynamic Model Identification for Insect Electroantennogram with Printed Electrode

Yamada¹,

Ohashi²,

Umedachi³

et al. 2021

Sensors and Materials

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The purpose of this study is to establish a method by which anyone can easily use an insect antenna as a robot olfactory sensor. Research has been conducted to use an insect antenna as a robot olfactory sensor because insect antennae are much more selective and sensitive than artificial odor sensors. To use an antenna as a robot olfactory sensor, a robot was controlled on the basis of the change in the electroantennogram (EAG) response to odor. However, EAG measurements were performed using microelectrodes or glass electrodes. Hence, it is difficult for engineering researchers and others who are not familiar with microscopic work to easily use an antenna as an odor sensor. For that reason, in this study, we designed a printed electrode using printed electronics (PE) technology that can be used for EAG measurement even for different morphologies of antennae. To detect the presence or absence of odor from the EAG response, we used a dynamic model to eliminate the effects of hum noise and drift associated with the measurement of biological signals. As a result, we proposed a method to make insect antennae easily usable for olfaction for an autonomous robot.This version has been created for advance publication by formatting the accepted manuscript. Some editorial changes may be made to this version.

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Section: Design Of Printed Electrodementioning

confidence: 99%