An insect chemosensory protein facilitates locust avoidance to fungal pathogens via recognition of fungal volatiles

Zheng, Renwen; Xie, Mushan; Keyhani, Nemat O.; Xia, Yuxian

doi:10.1016/j.ijbiomac.2023.127389

Cited by 3 publications

(1 citation statement)

References 59 publications

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“…Insects have acquired robust olfactory approaches over the course of long-term evolution, contributing to their behaviors, including host localization, mate location, and selection of egg-laying sites [1,2]. Insect olfaction is facilitated by several types of olfactory proteins, such as odorant-binding proteins (OBPs) [3], chemosensory proteins (CSPs) [4], odorant receptors (ORs) [5], ionotropic receptors (IRs) [6], sensory neuron membrane proteins (SNMPs) [7], gustatory receptors (GRs) [8], and odorant-degrading enzymes (ODEs) [9]. The OBPs recognize hydrophobic odor molecules and deliver them to ORs, which highlight the remarkable sensitivity of insect olfactory systems.…”

Section: Introductionmentioning

confidence: 99%

Molecular and Functional Characterization of Three General Odorant-Binding Protein 2 Genes in Cydia pomonella (Lepidoptera: Tortricidae)

Zhou,

Huang,

et al. 2024

IJMS

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General odorant-binding proteins (GOBPs) play a crucial role in the detection of host plant volatiles and pheromones by lepidopterans. Previous studies identified two duplications in the GOBP2 gene in Cydia pomonella. In this study, we employed qRT-PCR, protein purification, and fluorescence competitive binding assays to investigate the functions of three GOBP2 genes in C. pomonella. Our findings reveal that CpomGOBP2a and CpomGOBP2b are specifically highly expressed in antennae, while CpomGOBP2c exhibits high specific expression in wings, suggesting a potential divergence in their functions. Recombinant proteins of CpomGOBP2a, CpomGOBP2b, and CpomGOBP2c were successfully expressed and purified, enabling an in-depth exploration of their functions. Competitive binding assays with 20 host plant volatiles and the sex pheromone (codlemone) demonstrated that CpomGOBP2a exhibits strong binding to four compounds, namely butyl octanoate, ethyl (2E,4Z)-deca-2,4-dienoate (pear ester), codlemone, and geranylacetone, with corresponding dissolution constants (Ki) of 8.59993 μM, 9.14704 μM, 22.66298 μM, and 22.86923 μM, respectively. CpomGOBP2b showed specific binding to pear ester (Ki = 17.37481 μM), while CpomGOBP2c did not exhibit binding to any tested compounds. In conclusion, our results indicate a functional divergence among CpomGOBP2a, CpomGOBP2b, and CpomGOBP2c. These findings contribute valuable insights for the development of novel prevention and control technologies and enhance our understanding of the evolutionary mechanisms of olfactory genes in C. pomonella.

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Section: Introductionmentioning

confidence: 99%

Molecular and Functional Characterization of Three General Odorant-Binding Protein 2 Genes in Cydia pomonella (Lepidoptera: Tortricidae)

Zhou,

Huang,

et al. 2024

IJMS

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Syntaxin5 is essential for survival by ensuring midgut epithelial homeostsis and regulating feeding in Locusta migratoria

Liu,

Gao,

et al. 2024

Pesticide Biochemistry and Physiology

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Targeting Syntaxin 1A via RNA interference inhibits feeding and midgut development in Locusta migratoria

Liu,

Gao,

et al. 2024

Insect Science

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Syntaxin 1A (Syx1A) has diverse and indispensable functions in animals. Previous studies have mainly focused on the roles of Syx1A in Drosophila, and so how Syx1A operates during the development of other insects remains poorly understood. This study investigated whether disrupting LmSyx1A using RNA interference (RNAi) affects the growth and development of Locusta migratoria. LmSyx1A was expressed in all tissues tested, with the highest expression observed in the fat body. After 5th‐instar nymphs were injected with double‐stranded LmSyx1A (dsLmSyx1A), none of the nymphs were able to molt normally and all eventually died. The silencing of LmSyx1A resulted in the cessation of feeding, body weight loss, and atrophy of the midgut and gastric cecum in locusts. Hematoxylin and eosin (H&E) staining showed that the columnar cells in the midgut were severely damaged, with microvilli defects visible in dsLmSyx1A‐injected nymphs. Secretory vesicles were observed with transmission electron microscopy (TEM). In addition, reverse transcription quantitative polymerase chain reaction (RT‐qPCR) further indicates that silencing LmSyx1A repressed the expression of genes involved in the insulin/mammalian target of rapamycin (mTOR)‐associated nutritional pathway. Taken together, these results suggest that LmSyx1A significantly affects the midgut cell layer of locust nymphs, which was partially associated with the downregulation of the insulin/mTOR‐associated nutritional pathway. Thus, we argue that LmSyx1A is a suitable target for developing dsRNA‐based biological pesticides for managing L. migratoria.

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An insect chemosensory protein facilitates locust avoidance to fungal pathogens via recognition of fungal volatiles

Cited by 3 publications

References 59 publications

Molecular and Functional Characterization of Three General Odorant-Binding Protein 2 Genes in Cydia pomonella (Lepidoptera: Tortricidae)

Molecular and Functional Characterization of Three General Odorant-Binding Protein 2 Genes in Cydia pomonella (Lepidoptera: Tortricidae)

Syntaxin5 is essential for survival by ensuring midgut epithelial homeostsis and regulating feeding in Locusta migratoria

Targeting Syntaxin 1A via RNA interference inhibits feeding and midgut development in Locusta migratoria

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