Silencing Of Circular RNA-ZNF609 Ameliorates Vascular Endothelial Dysfunction

Liu, Chang; Yao, Mengfei; Li, Chaopeng; Shan, K. Y.; Yang, Hong; Wang, Jia-Jian; Liu, Ban; Li, Xiu‐Miao; Yao, Jin; Jiang, Qin; Yan, Biao

doi:10.7150/thno.19353

Cited by 222 publications

(177 citation statements)

References 39 publications

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“…Accordingly, an increasing number of studies has revealed that the circRNA‐miRNA‐target gene regulatory network might have profound implications for understanding diseases, especially cancers . For example, a recent study shows that circ‐ZNF609 acts as an endogenous miR‐615‐5p sponge to inhibit miR‐615‐5p activity, increasing myocyte‐specific enhancer factor 2A (MEF2A) expression and ameliorating vascular endothelial dysfunction . Zhong et al found that circ‐MYLK is not only related to the stage and grade of the cancer but can also work with miR‐29a and relieve the suppression of target vascular endothelial growth factor A ( VEGFA ), a key member of the VEGFA/VEGFR2/Ras/ERK signaling pathway.…”

Section: Functions Of Circrnasmentioning

confidence: 99%

Circular RNAs in hepatocellular carcinoma: Functions and implications

Jiang

et al. 2018

Cancer Medicine

108

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At present, as hotspot members of the noncoding RNA network, circular RNAs (circRNAs) with distinct properties and diverse pathophysiological functions are being increasingly delineated. CircRNAs play roles at the epigenetic, transcriptional and posttranscriptional regulatory levels. Major studies have focused on their functions as efficient microRNA sponges. The validated number of endogenous circRNAs involved in hepatocellular carcinoma (HCC) continues to increase. Altered circRNA expression is associated with HCC occurrence, invasion, and metastasis. Moreover, the aberrant expression of circRNAs is also significantly related to HCC tumor stage, size, differentiation and metastasis. Because they are exceptionally stable, highly conserved and have tissue‐specific expression patterns, some circRNAs, including hsa_circ_0004018, hsa_circ_0003570, and hsa_circ_0005075, may be potential markers for the diagnosis of HCC. We herein summarize the current knowledge of HCC‐associated circRNAs and present their implications for carcinogenesis and their potential value as diagnostic and prognostic biomarkers. Finally, we discuss the future directions of studies on HCC‐associated circRNAs.

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Section: Functions Of Circrnasmentioning

confidence: 99%

Circular RNAs in hepatocellular carcinoma: Functions and implications

Jiang

et al. 2018

Cancer Medicine

108

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“…[6][7][8] Knockdown of cZNF609, which is highly enriched in endothelial cells, diminished retinal vessel loss and inhibited pathological angiogenesis in vivo. 9 CircRNA USP1 (circ-USP1, also known as hsa_circ_0000080 according to circBase), which is located at chr1p31, is derived from the back-splicing of exon 6-8 of ubiquitin-specific peptidase 1 (USP1) gene. Circ-USP1 is significantly upregulated in our microarray screen detected by comparing human cerebral microvascular endothelial cell line (ECs) with endothelia cells co-cultured with glioma (GECs).…”

mentioning

confidence: 99%

Circular RNA USP1 regulates the permeability of blood‐tumour barrier via miR‐194‐5p/FLI1 axis

Gao

et al. 2019

J Cellular Molecular Medi

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Recent studies indicate circular RNAs are related to dysregulation of vascular endothelial cell function, yet the underlying mechanisms have remained elusive. Here, we characterized the functional role of circular RNA USP1 (circ‐USP1) in the regulation of the blood‐tumour barrier (BTB) permeability and the potential mechanisms. In the current study, the circ‐USP1 expressing level was up‐regulated in glioma cerebral microvascular endothelial cells (GECs) of the BTB model in vitro. Knockdown of circ‐USP1 disrupted the barrier integrity, increased its permeability as well as reduced tight junction‐related protein claudin‐5, occludin and ZO‐1 expressions in GECs. Bioinformatic prediction and luciferase assay indicated that circ‐USP1 bound to miR‐194‐5p and suppressed its activity. MiR‐194‐5p contributed to circ‐USP1 knockdown‐induced increase of BTB permeability via targeting and down‐regulating transcription factor FLI1. Furthermore, FLI1 regulated the expressions of claudin‐5, occludin and ZO‐1 in GECs through binding to their promoter regions. Single or combined treatment of circ‐USP1 and miR‐194‐5p effectively promoted anti‐tumour drug doxorubicin across BTB to induce apoptosis of glioma cells. Overall, this present study identified the crucial regulation of circ‐USP1 on BTB permeability via miR‐194‐5p/FLI1 axis‐mediated regulation of tight junction proteins, which might facilitate the development of therapeutics against human gliomas.

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“…Loss‐of‐function experiments have shown that hsa‐circ‐0003575 silencing promoted the proliferation and angiogenesis ability of OX‐LDL–induced ECs. In addition, circ‐ZNF609 silencing can also increase EC migration and vascular formation . Besides, circ‐ZNF609‐miR‐615‐5p‐MEF2A network was confirmed subsequently to be the main signaling pathway that is involved in regulating endothelial cell function.…”

Section: Targets Of Noncoding Rnas In Cardiovascular Disease With Diamentioning

confidence: 85%

Noncoding RNAs as therapeutic targets in atherosclerosis with diabetes mellitus

Tang

Jiang

Yang

et al. 2018

Cardiovascular Therapeutics

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Atherosclerosis is one of the major macrovascular complications of diabetes mellitus (DM), and it is the main cause of death from clinical observation. Among various cell types involved in this disorder, endothelial cells, vascular smooth muscle cells (VSMCs), and macrophages play a crucial role in the occurrence and development of this disease. The regulation and stabilization of these cells are a key therapeutic strategy for DM-associated atherosclerosis. An increasing number of evidences implicate that various types of noncoding RNAs (ncRNAs) play a vital role in many cellular responses as well as in physiological and pathological processes of atherosclerosis and DM that drive atherogenic/antiatherogenic processes in those cells. Encouragingly, many ncRNAs have already been tested in animal experiments or clinical trials showing good performance. In this review, we summarize recent progresses in research on functional regulatory role of ncRNAs in atherosclerosis with DM. More importantly, we illustrate new thoughts and findings relevant to ncRNAs as potential therapeutic targets or biomarkers for atherosclerosis with DM.

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Silencing Of Circular RNA-ZNF609 Ameliorates Vascular Endothelial Dysfunction

Cited by 222 publications

References 39 publications

Circular RNAs in hepatocellular carcinoma: Functions and implications

Circular RNAs in hepatocellular carcinoma: Functions and implications

Circular RNA USP1 regulates the permeability of blood‐tumour barrier via miR‐194‐5p/FLI1 axis

Noncoding RNAs as therapeutic targets in atherosclerosis with diabetes mellitus

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