Circular RNA USP1 regulates the permeability of blood‐tumour barrier via miR‐194‐5p/FLI1 axis

Gao, Yang; Wu, Peiqi; Ma, Yuhang; Xue, Yixue; Liu, Yunhui; Zheng, Jian; Liu, Xiaobai; He, Qianru; Ma, Jun; Liu, Libo; Wang, Ping

doi:10.1111/jcmm.14735

Cited by 13 publications

(11 citation statements)

References 45 publications

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“…Circ-DLGAP4 can promote HECTD1 expression by sponging miR-143 and enhance BBB function 183 . As a miR-194-5p sponge, circ-ubiquitin specific peptidase 1 (USP1) relieves the inhibitory effect of miR-194-5p on occludin, ZO-1, and claudin-5 mRNAs and decreases the BBTB permeability 184 . CircRNAs are stable and have potential as molecular markers and drug targets, and their relationships with TJs deserve more extensive and in-depth researches.…”

Section: Circrnas and Tjsmentioning

confidence: 99%

Tight junctions and their regulation by non-coding RNAs

Zhao¹,

Zeng²,

Li³

et al. 2021

Int. J. Biol. Sci.

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Tight junction (TJ) is a “zippering up” junction structure located at the uppermost portion of adjacent epithelial/endothelial cells in organs and tissues. TJs maintain the relative stability of intracellular substances and functions by closing or opening intercellular pathways, coordinating the entry and exit of molecules of different sizes and charges, and regulating the permeability of paracellular barrier. TJs also prevent microbial invasion, maintain epithelial/endothelial cell polarity, and regulate cell proliferation. TJs are widely present in the skin and mucosal epithelial barriers, intestinal epithelial barrier, glomerular filtration barrier, bladder epithelial barrier, blood-brain barrier, brain-blood tumor barrier, and blood-testis barrier. TJ dysfunction in different organs can lead to a variety of diseases. In addition to signal pathways, transcription factors, DNA methylation, histone modification, TJ proteins can also be regulated by a variety of non-coding RNAs, such as micro-RNAs, long-noncoding RNAs, and circular RNAs, directly or indirectly. This review summarizes the structure of TJs and introduces the functions and regulatory mechanisms of TJs in different organs and tissues. The roles and mechanisms of non-coding RNAs in the regulation of TJs are also highlighted in this review.

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Section: Circrnas and Tjsmentioning

confidence: 99%

Tight junctions and their regulation by non-coding RNAs

Zhao¹,

Zeng²,

Li³

et al. 2021

Int. J. Biol. Sci.

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“…The results showed that the expression of circ‐USP1 was elevated in renal allografts in a time‐dependent manner. Notably, the expression of miR‐194‐5p, a previously reported circ‐USP1 target, 19 decreased in diseased kidneys. Additionally, silencing circ‐USP1 in mice protected them from kidney injury following a renal allograft.…”

Section: Introductionmentioning

confidence: 84%

“…Although recent reports indicate that circ‐USP1 is involved in the dysregulation of endothelial cell functions, 19 its role in kidney diseases remains largely unclear. Gao et al found that silencing circ‐USP increased permeability of the blood‐tumour barrier by decreasing tight junction‐related protein expression in cerebral microvascular endothelial cells in glioma 19 . In this study, it was shown that silencing the circ‐USP1 protects renal tubule cells from apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, recent evidence shows that miR‐194‐5p is involved in the progression of various diseases including acute myeloid leukaemia, clear cell kidney carcinoma and hepatocellular carcinoma 16‐18 . A previous study showed that miR‐194‐5p was a target of circ‐USP1 and both were involved in regulating the permeability of the blood‐tumour barrier 19 . Nevertheless, to the best of our knowledge, the role of miR‐194‐5p and circ‐USP1 in the progression of kidney injury induced by hypoxia in acute renal allografts remains unknown.…”

Section: Introductionmentioning

confidence: 99%

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Silencing circ‐USP1 protects the renal tubular from kidney injury induced by hypoxia via modulating miR‐194‐5p/DNMT3A axis in acute renal allografts

Yao

Zha

Gao

et al. 2021

J Cellular Molecular Medi

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Recent studies indicate that circular RNAs are involved in dysregulation of kidney injury. Nevertheless, the underlying mechanisms remain largely unclear. Therefore, this study sought to investigate the role of circ‐USP1 in the pathogenesis of early renal allografts. Thirty‐two male C57BL/6J mice aged between 6 and 8 weeks were divided into the sham and allograft groups. Thereafter, the association between miR‐194‐5p, circ‐USP1 and DNMT3A was confirmed using a combination of bioinformatics and the luciferase reporter gene assay. Additionally, the expression of circ‐USP1, miR‐194‐5p and DNMT3A mRNA was detected through qPCR. Afterwards, the Western blot assay was performed to examine the expression of DNMT3A protein. Finally, the TUNEL assay was conducted to determine the rate of apoptosis in DNMT3A cells. The expression of circ‐USP1 increased, while that of miR‐194‐5p decreased in renal allografts. Additionally, silencing circ‐USP1 reduced kidney injuries caused by renal allografts in mice. Moreover, miR‐194‐5p was a target for circ‐USP1, and DNMT3A was a target of miR‐194‐5p. Finally, it was shown that silencing circ‐USP1 reduced DNMT3A expression in the kidney of mice that received renal allografts. Circ‐USP1 functions as a competing endogenous RNA for miR‐194‐5p. This occurs in order to regulate DNMT3A expression in kidney injury induced by hypoxia in acute renal allografts.

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“…For example, miR-194-5p regulates FOXA1 by targeting and reversing the apoptosis and cell cycle arrest of hepatocellular carcinoma induced by MCM3AP-AS1 silencing 61 . miR-194-5p binds to FLI1 and downregulates its expression to increase the permeability of the blood tumour barrier 62 . In the current study, based on the application of biological information software starBase to predict the binding site of Runx3 and miR-194-5p, the dual-luciferase reporter gene experiment confirmed Runx3 as the target gene of miR-194-5p.…”

Section: Discussionmentioning

confidence: 99%

Effect of circular RNA, mmu_circ_0000296, on neuronal apoptosis in chronic cerebral ischaemia via the miR-194-5p/Runx3/Sirt1 axis

et al. 2021

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Chronic cerebral ischaemia (CCI) is a common pathological disorder, which is associated with various diseases, such as cerebral arteriosclerosis and vascular dementia, resulting in neurological dysfunction. As a type of non-coding RNA, circular RNA is involved in regulating the occurrence and development of diseases, such as ischaemic brain injury. Here, we found that HT22 cells and hippocampus treated with CCI had low expression of circ_0000296, Runx3, Sirt1, but high expression of miR-194-5p. Overexpression of circ_0000296, Runx3, Sirt1, and silenced miR-194-5p significantly inhibited neuronal apoptosis induced by CCI. This study demonstrated that circ_0000296 specifically bound to miR-194-5p; miR-194-5p bound to the 3′UTR region of Runx3 mRNA; Runx3 directly bound to the promoter region of Sirt1, enhancing its transcriptional activity. Overexpression of circ_0000296 by miR-194-5p reduced the negative regulatory effect of miR-194-5p on Runx3, promoted the transcriptional effect of Runx3 on Sirt1, and inhibited neuronal apoptosis induced by CCI. mmu_circ_0000296 plays an important role in regulating neuronal apoptosis induced by CCI through miR-194-5p/Runx3/Sirt1 pathway.

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Circular RNA USP1 regulates the permeability of blood‐tumour barrier via miR‐194‐5p/FLI1 axis

Cited by 13 publications

References 45 publications

Tight junctions and their regulation by non-coding RNAs

Tight junctions and their regulation by non-coding RNAs

Silencing circ‐USP1 protects the renal tubular from kidney injury induced by hypoxia via modulating miR‐194‐5p/DNMT3A axis in acute renal allografts

Effect of circular RNA, mmu_circ_0000296, on neuronal apoptosis in chronic cerebral ischaemia via the miR-194-5p/Runx3/Sirt1 axis

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