Effect of circular RNA, mmu_circ_0000296, on neuronal apoptosis in chronic cerebral ischaemia via the miR-194-5p/Runx3/Sirt1 axis

Abstract

Chronic cerebral ischaemia (CCI) is a common pathological disorder, which is associated with various diseases, such as cerebral arteriosclerosis and vascular dementia, resulting in neurological dysfunction. As a type of non-coding RNA, circular RNA is involved in regulating the occurrence and development of diseases, such as ischaemic brain injury. Here, we found that HT22 cells and hippocampus treated with CCI had low expression of circ_0000296, Runx3, Sirt1, but high expression of miR-194-5p. Overexpression of circ_0000296, Runx3, Sirt1, and silenced miR-194-5p significantly inhibited neuronal apoptosis induced by CCI. This study demonstrated that circ_0000296 specifically bound to miR-194-5p; miR-194-5p bound to the 3′UTR region of Runx3 mRNA; Runx3 directly bound to the promoter region of Sirt1, enhancing its transcriptional activity. Overexpression of circ_0000296 by miR-194-5p reduced the negative regulatory effect of miR-194-5p on Runx3, promoted the transcriptional effect of Runx3 on Sirt1, and inhibited neuronal apoptosis induced by CCI. mmu_circ_0000296 plays an important role in regulating neuronal apoptosis induced by CCI through miR-194-5p/Runx3/Sirt1 pathway.

Expression levels of RUNX3 and FGFR2 in peripheral blood of severe acute pancreatitis and their clinical significance

“…As indicated by a previous study, RUNX3 is a transcription activator of its downstream genes [ 13 ]. Through the JASPAR database, RUNX3 was predicted to bind to the FGFR2 promoter ( Fig.…”

“…Beyond that, FGFR2 overexpression alleviates apoptosis and inflammatory responses in caerulein-treated pancreatic duct epithelial cells [ 12 ]. RUNX3 as a transcription factor can bind to the gene promoter to promote the transcription of downstream genes [ 13 ], and the JASPAR database ( http://jaspar.genereg.net/ ) further predicted the binding site of RUNX3 and FGFR2 , hinting at the regulation of RUNX3 on FGFR2 expression. Nevertheless, expression patterns of RUNX3 and FGFR2 in peripheral blood of SAP patients, their diagnostic values in SAP, and whether RUNX3 can regulate FGFR2 expression in SAP have not been reported before.…”