2019
DOI: 10.1021/acs.jafc.9b02317
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Silencing TT8 and HB12 Decreased Protein Degradation and Digestion, Microbial Synthesis, and Metabolic Protein in Relation to Molecular Structures of Alfalfa (Medicago sativa)

Abstract: This study aimed to explore the effects of silencing HB12 and TT8 genes on protein utilization characteristics of alfalfa. Ground samples of 11 HB12-silenced (HB12i), 5 TT8-silenced (TT8i) and 4 wild type (WT) were incubated in a Daisy II incubator with N15 labeled ammonium sulfate for 0, 4, 8, 12, and 24 h. CP degradation and degradational kinetics, microbial nitrogen fractions, and protein metabolic profiles were determined. Moreover, relationships between protein profiles and FTIR spectral parameters were e… Show more

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Cited by 5 publications
(4 citation statements)
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“…The identification of these lignin synthesis genes will promote the development of low-lignin alfalfa cultivars. As reported, a low-lignin alfalfa cultivar, KK179, characterized by its down-regulation of CCoAOMT , has been approved in many countries [ 42 ]. Here, a total of 12 differentially expressed genes, including PAL , 4CL , HCT , CAD , COMT and POD , were identified in four developmental stages between the cultivars.…”
Section: Discussionmentioning
confidence: 99%
“…The identification of these lignin synthesis genes will promote the development of low-lignin alfalfa cultivars. As reported, a low-lignin alfalfa cultivar, KK179, characterized by its down-regulation of CCoAOMT , has been approved in many countries [ 42 ]. Here, a total of 12 differentially expressed genes, including PAL , 4CL , HCT , CAD , COMT and POD , were identified in four developmental stages between the cultivars.…”
Section: Discussionmentioning
confidence: 99%
“…Alfalfa plant samples were obtained from the Agriculture and Agri-Food Canada-Saskatoon Research and Development Centre (Saskatoon, Canada). The detailed processes of alfalfa transformation, growth conditions and harvest information were previously described ( Li et al., 2015 , Lei et al., 2018a , 2018b , 2019 ). Briefly, to make RNAi constructs, cDNA was synthesized from total alfalfa RNA that was extracted from the fourth internode.…”
Section: Methodsmentioning
confidence: 99%
“…Transparent Testa ( TT8 ) and Homeobox 12 ( HB12 ) are two transcriptional factors in this pathway. Observation from Brassica napus showed a negative relationship between lignin content and gene expression of TT8 and HB12 ( Li et al., 2016a ; Lei et al., 2018a , 2018b , 2019 ). Therefore, we transformed alfalfa with TT8 and HB12 RNAi constructs to silence these 2 genes in alfalfa.…”
Section: Introductionmentioning
confidence: 99%
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