Comparative transcriptome analyses reveal that the MsNST1 gene affects lignin synthesis in alfalfa (Medicago sativa L.)

Zhou, Qiang; Mao, Pei; Luo, Dong; Chai, Xutian; Deng, Hao; Fang, Qiangen; Fang, Lichun; Zhang, Nan; Wen, Jiangqi; Liu, Wenxian

doi:10.1016/j.cj.2022.02.002

Cited by 5 publications

(4 citation statements)

References 66 publications

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“…It is worth mentioning that several genes in the phenylpropanoid biosynthesis pathway were noticed when analysing KEGG classification map phase [23]. As the second-mostabundant natural polymer, lignin plays an essential role in plant development.…”

Section: Discussionmentioning

confidence: 99%

Molecular Regulation of Bud Regeneration from Callus of Hybrid Sweetgum (Liquidambar styraciflua × Liquidambar formosana)

Ai,

Ma,

Zhang

et al. 2023

Forests

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Adventitious bud regeneration is an effective means of rapid propagation of plants, which can be used in the study of plant development and genetic transformation. It can be divided into direct and indirect adventitious bud regeneration. Of the two kinds of adventitious bud regeneration mentioned, indirect adventitious bud regeneration includes callus formation in vitro and organ regeneration. In the process of callus formation, some cells acquire the pluripotency of tissue regeneration, which is the key to regeneration of adventient buds. It is not clear which molecular processes and genetic factors are involved in establishing cellular pluripotency. The object of the study is hybrid sweetgum, whose mother is Liquidambar styraciflua and father is Liquidambar formosana. At present, the reproductive efficiency of hybrid sweetgum is low and the reproductive cycle is long. Improving its reproductive efficiency by improving the differentiation speed of callus may be a decent approach. In order to explore the mechanism of pluripotency acquisition during forming hybrid sweetgum callus, we used RNA-seq to perform transcriptomic analysis of the regenerable calli (RC) and non-regenerable (NRC) calli of hybrid sweetgum. A dataset of differentially expressed genes (DEG) was obtained and several genes probably involved in bud formation were analyzed to explain the molecular processes of acquiring and maintaining pluripotency. In this study, a total of 665 significantly expressed DEGs were identified in the RC and NRC of hybrid sweetgum, among which, 585 differentially expressed genes were up-regulated and 80 differentially expressed genes were down-regulated.

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Section: Discussionmentioning

confidence: 99%

Molecular Regulation of Bud Regeneration from Callus of Hybrid Sweetgum (Liquidambar styraciflua × Liquidambar formosana)

Ai,

Ma,

Zhang

et al. 2023

Forests

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“…To examine the lignification of the MsDREB1C overexpression alfalfa stem, histological analysis using the Wiesner test [12] was conducted. The tenth internode from the top of alfalfa at the 6-week stage was sectioned and dyed using phloroglucinol-HCl to detect the pink stain of lignifying xylem cell walls.…”

Section: Overexpression Of Msdreb1c In Alfalfa Improves Forage Qualit...mentioning

confidence: 99%

“…Fresh samples of the tenth stem segments, counting from the top of the 6-week-old plant, were cross-sectioned into 20-25 µm thin slices and stained with 2% phloroglucinol staining solution after dewaxing and hydration according to a previous method [12]. The sealed sample was imaged under an optical microscope (Olympus microscope CX-21, Tokyo, Japan).…”

Section: Lignification Analysismentioning

confidence: 99%

“…Therefore, to ensure alfalfa yield, screening germplasms with a high leaf-stem ratio (LSR) and crude protein content is crucial to improve forage quality and reduce fertilizer application in order to promote sustainable agricultural development. In recent years, evidence has shown that by regulating the key synthetic genes of cellulose, such as caffeoyl CoA 3-Omethyltransferase (CCoAOMT) [9][10][11] and NAC SECONDARY WALL THICKENING PROMOTING FACTOR (NST) [12], the cellulose content of alfalfa can be reduced, thus laying the foundation for low-cellulose alfalfa breeding. In addition, studies have also shown that regulating the flowering time by regulating the expression of the MsFT gene can also improve the quality and yield of alfalfa [2,13].…”

Section: Introductionmentioning

confidence: 99%

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Overexpression of MsDREB1C Modulates Growth and Improves Forage Quality in Tetraploid Alfalfa (Medicago sativa L.)

Zhang,

Wang,

Zhang

et al. 2024

Plants

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DREB has been reported to be involved in plant growth and response to environmental factors. However, the function of DREB in growth and development has not been elucidated in alfalfa (Medicago sativa L.), a perennial tetraploid forage cultivated worldwide. In this study, an ortholog of MtDREB1C was characterized from alfalfa and named MsDREB1C accordingly. MsDREB1C was significantly induced by abiotic stress. The transcription factor MsDREB1C resided in the nucleus and had self-transactivation activity. The MsDREB1C overexpression (OE) alfalfa displayed growth retardation under both long-day and short-day conditions, which was supported by decreased MsGA20ox and upregulated MsGA2ox in the OE lines. Consistently, a decrease in active gibberellin (GA) was detected, suggesting a negative effect of MsDREB1C on GA accumulation in alfalfa. Interestingly, the forage quality of the OE lines was better than that of WT lines, with higher crude protein and lower lignin content, which was supported by an increase in the leaf–stem ratio (LSR) and repression of several lignin-synthesis genes (MsNST, MsPAL1, MsC4H, and Ms4CL). Therefore, this study revealed the effects of MsDREB1C overexpression on growth and forage quality via modifying GA accumulation and lignin synthesis, respectively. Our findings provide a valuable candidate for improving the critical agronomic traits of alfalfa, such as overwintering and feeding value of the forage.

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Genome-Wide Identification of COMT Gene Family in Maize and its Function in Response to Light

Lei,

Chen,

et al. 2024

Biochem Genet

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Comparative transcriptome analyses reveal that the MsNST1 gene affects lignin synthesis in alfalfa (Medicago sativa L.)

Cited by 5 publications

References 66 publications

Molecular Regulation of Bud Regeneration from Callus of Hybrid Sweetgum (Liquidambar styraciflua × Liquidambar formosana)

Molecular Regulation of Bud Regeneration from Callus of Hybrid Sweetgum (Liquidambar styraciflua × Liquidambar formosana)

Overexpression of MsDREB1C Modulates Growth and Improves Forage Quality in Tetraploid Alfalfa (Medicago sativa L.)

Genome-Wide Identification of COMT Gene Family in Maize and its Function in Response to Light

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