Silenced rice in both cytosolic ascorbate peroxidases displays pre-acclimation to cope with oxidative stress induced by 3-aminotriazole-inhibited catalase

Bonifácio, Aurenívia; Carvalho, Fernanda Torres de; Martins, Márcio O.; Neto, Milton Costa Lima; Cunha, Juliana R.; Ribeiro, C. T. M.; Margis‐Pinheiro, Márcia; Silveira, Joaquim Albenísio Gomes da

doi:10.1016/j.jplph.2016.06.015

Cited by 35 publications

(25 citation statements)

References 69 publications

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“…Although TRX h2 is a nonplastid protein, there is evidence from previous studies that alteration of genes involved in cytosolic and mitochondrial redox metabolism can impair photosynthesis (Bonifacio et al, ; Carvalho et al, ; Lima‐Melo et al, ). We thus decided to investigate whether the lack of TRX h 2 impacts photosynthesis in Arabidopsis through the analysis of photochemical and gas exchange parameters under different light and CO 2 conditions.…”

Section: Resultsmentioning

confidence: 99%

Thioredoxin h2 contributes to the redox regulation of mitochondrial photorespiratory metabolism

Fonseca‐Pereira

Souza

Hou

et al. 2019

Plant Cell & Environment

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Thioredoxins (TRXs) are important proteins involved in redox regulation of metabolism. In plants, it has been shown that the mitochondrial metabolism is regulated by the mitochondrial TRX system. However, the functional significance of TRX h2, which is found at both cytosol and mitochondria, remains unclear. Arabidopsis plants lacking TRX h2 showed delayed seed germination and reduced respiration alongside impaired stomatal and mesophyll conductance, without impacting photosynthesis under ambient O2 conditions. However, an increase in the stoichiometry of photorespiratory CO2 release was found during O2‐dependent gas exchange measurements in trxh2 mutants. Metabolite profiling of trxh2 leaves revealed alterations in key metabolites of photorespiration and in several metabolites involved in respiration and amino acid metabolism. Decreased abundance of serine hydroxymethyltransferase and glycine decarboxylase (GDC) H and L subunits as well as reduced NADH/NAD+ ratios were also observed in trxh2 mutants. We further demonstrated that the redox status of GDC‐L is altered in trxh2 mutants in vivo and that recombinant TRX h2 can deactivate GDC‐L in vitro, indicating that this protein is redox regulated by the TRX system. Collectively, our results demonstrate that TRX h2 plays an important role in the redox regulation of mitochondrial photorespiratory metabolism.

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Section: Resultsmentioning

confidence: 99%

Thioredoxin h2 contributes to the redox regulation of mitochondrial photorespiratory metabolism

Fonseca‐Pereira

Souza

Hou

et al. 2019

Plant Cell & Environment

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“…A plausible reason is that Cd stress causes cellular damage and peroxidative loss of free radicals in rape leaves; these lead to intracellular lipid peroxidation of intracellular membranes and initiates the stress defense mechanisms, thereby causing the increases in MDA and proline contents in rape. The contents of MDA and proline decreased in rape leaves under combined Mo application, which proves that Mo can effectively reduce Cd-induced damage of intracellular membranes in rape [46,47].…”

Section: Exogenous Mo Benefits Plant Physiology and Biomass In Cd-strmentioning

confidence: 95%

Effect of Molybdenum on Plant Physiology and Cadmium Uptake and Translocation in Rape (Brassica napus L.) under Different Levels of Cadmium Stress

Han

Wang

Wan

et al. 2020

IJERPH

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This study investigated the beneficial effect of molybdenum (Mo) application on rape plants (Brassica napus L.) grown in a soil polluted by cadmium (Cd). A pot experiment was conducted to determine how different concentrations of exogenous Mo (0, 50, 100, and 200 mg/kg) affect plant physiology, biomass, photosynthesis, cation uptake, and Cd translocation and enrichment in rape plants under Cd stress (0.5 and 6.0 mg/kg). Under single Cd treatment, plant physiological and biochemical parameters, biomass parameters, leaf chlorophyll fluorescence parameters, and macroelement uptake of rape plants decreased, while their malonaldehyde content, proline content, non-photochemical quenching coefficient, and Cd uptake significantly increased, compared to those of the control group (p-values < 0.05). High-Cd treatment resulted in much larger changes in these parameters than low-Cd treatment. Following Mo application, the accumulation of malondialdehyde and proline decreased in the leaves of Cd-stressed plants; reversely, the contents of soluble protein, soluble sugar, and chlorophyll, and the activities of superoxide dismutase and glutathione peroxidase, all increased compared to those of single Cd treatment (p-values < 0.05). Exogenous Mo application promoted shoot and root growth of Cd-stressed plants in terms of their length, fresh weight, and dry weight. The negative effect of Cd stress on leaf chlorophyll fluorescence was substantially mitigated by applying Mo. Exogenous Mo also improved the uptake of inorganic cations, especially potassium (K+), in Cd-stressed plants. After Mo application, Cd uptake and accumulation were inhibited and Cd tolerance was enhanced, but Cd translocation was less affected in Cd-stressed plants. The mitigation effect of Mo on Cd stress in rape was achieved through the immobilization of soil Cd to reduce plant uptake, and improvement of plant physiological properties to enhance Cd tolerance. In conclusion, exogenous Mo can effectively reduce Cd toxicity to rape and the optimal Mo concentration was 100 mg/kg under the experimental conditions.

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“…As H 2 O 2 changes gene expression and directly affects the function of specific proteins by cysteine oxidation, we must suppose that this results in acclimation to potentially toxic H 2 O 2 exposure and related stresses. Various studies with APX and CAT mutants, most recently in rice, suggest that, under some conditions, these are more resistant to oxidative stress, consistent with acclimation (Bonifacio et al ., ). Specifically, H 2 O 2 pretreatment increased tobacco resistance to CAT inhibition and high light (Gechev et al ., ).…”

Section: H2o2 Signallingmentioning

confidence: 97%

Hydrogen peroxide metabolism and functions in plants

2018

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1 I. Introduction 2 II. Measurement and imaging of H O 2 III. H O and O toxicity 3 IV. Production of H O : enzymes and subcellular locations 4 V. H O transport 9 VI. Control of H O concentration: how and where? 9 VII. Metabolic functions of H O 11 VIII. H O signalling 11 IX. Where next? 13 Acknowledgements 13 References 13 SUMMARY: Hydrogen peroxide (H O ) is produced, via superoxide and superoxide dismutase, by electron transport in chloroplasts and mitochondria, plasma membrane NADPH oxidases, peroxisomal oxidases, type III peroxidases and other apoplastic oxidases. Intracellular transport is facilitated by aquaporins and H O is removed by catalase, peroxiredoxin, glutathione peroxidase-like enzymes and ascorbate peroxidase, all of which have cell compartment-specific isoforms. Apoplastic H O influences cell expansion, development and defence by its involvement in type III peroxidase-mediated polymer cross-linking, lignification and, possibly, cell expansion via H O -derived hydroxyl radicals. Excess H O triggers chloroplast and peroxisome autophagy and programmed cell death. The role of H O in signalling, for example during acclimation to stress and pathogen defence, has received much attention, but the signal transduction mechanisms are poorly defined. H O oxidizes specific cysteine residues of target proteins to the sulfenic acid form and, similar to other organisms, this modification could initiate thiol-based redox relays and modify target enzymes, receptor kinases and transcription factors. Quantification of the sources and sinks of H O is being improved by the spatial and temporal resolution of genetically encoded H O sensors, such as HyPer and roGFP2-Orp1. These H O sensors, combined with the detection of specific proteins modified by H O , will allow a deeper understanding of its signalling roles.

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Silenced rice in both cytosolic ascorbate peroxidases displays pre-acclimation to cope with oxidative stress induced by 3-aminotriazole-inhibited catalase

Cited by 35 publications

References 69 publications

Thioredoxin h2 contributes to the redox regulation of mitochondrial photorespiratory metabolism

Thioredoxin h2 contributes to the redox regulation of mitochondrial photorespiratory metabolism

Effect of Molybdenum on Plant Physiology and Cadmium Uptake and Translocation in Rape (Brassica napus L.) under Different Levels of Cadmium Stress

Hydrogen peroxide metabolism and functions in plants

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