Sildenafil enhances the peripheral antinociceptive effect of ellagic acid in the rat formalin test

Mansouri, Mohammad Taghi; Naghizadeh, Bahareh; Ghorbanzadeh, Behnam

doi:10.4103/0253-7613.135952

Cited by 8 publications

(4 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The formalin test is widely used as a model of acute inflammatory pain, the closest test to clinical pain to evaluate antinociceptive activity of chemical compounds. Formalin injection activates peripheral sensory nerves and produces pain responses that involve ongoing peripheral activity and peripheral and central sensitization . The early phase of the formalin test represents the transmission of nociceptive impulses while second or late phase of the formalin test represents the events of central sensitization …”

Section: Introductionmentioning

confidence: 99%

Natural antioxidant flavonoids in formalin‐induced mice paw inflammation; inhibition of mitochondrial sorbitol dehydrogenase activity

Hasan

Mohamed

2017

J Biochem & Molecular Tox

View full text Add to dashboard Cite

Flavonoids have reported to cover interesting multiple pharmacological properties. This study evaluated the effect of apigenin or silymarin in paw inflammation induced by formalin in mice. Mice were divided into four groups: I: positive control group; II: apigenin, 3 mg/kg (i.p.); III: silymarin 50 mg/kg (p.o.); IV: meloxicam 10 mg/kg (p.o.), the reference drug. Therapies were administered once a day for 7 days. The curative effects were assessed on inflammatory, oxidative stress and neurotransmitter biomarkers, and apoptosis. Both flavonoids induced marked improvement in paw licking time, paw edema %, malondialdehyde content, superoxide dismutase, and sorbitol dehydrogenase activities, with slight progress in paw interlukin-1β. Additionally, silymarin augmented brain content of dopamine and norepinephrine. Furthermore, flavonoids induced marked decline in extent of apoptosis. So, the results spotlight on the good influence of apigenin or silymarin as anti-inflammatory, antioxidant, and antiapoptotic agents in formalin-induced mice paw inflammation to even a better extent than meloxicam.

show abstract

Section: Introductionmentioning

confidence: 99%

Natural antioxidant flavonoids in formalin‐induced mice paw inflammation; inhibition of mitochondrial sorbitol dehydrogenase activity

Hasan

Mohamed

2017

J Biochem & Molecular Tox

View full text Add to dashboard Cite

show abstract

“…[ 8 ] Extracts from red raspberry leaves or seeds, pomegranates, or various other sources containing high levels of EA are commercially available as dietary supplements. This compound exhibits anti-carcinogenic,[ 9 ] anti-oxidative,[ 10 ] antinociceptive and anti-inflammatory[ 11 12 ] activities. Furthermore, it has been shown that EA suppress the release of NO and prostaglandine E 2 (PGE 2 ) by down-regulating inducible NOS and COX, respectively in the endothelial cells and also rat model of colon cancer.…”

Section: Introductionmentioning

confidence: 99%

A study of the mechanisms underlying the anti-inflammatory effect of ellagic acid in carrageenan-induced paw edema in rats

et al. 2015

Self Cite

View full text Add to dashboard Cite

Objectives:Ellagic acid (EA) has shown antinociceptive and anti-inflammatory effects. Inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2) enzymes and also cytokines play a key role in many inflammatory conditions. This study was aimed to investigate the mechanisms involved in the anti-inflammatory effect of EA.Materials and Methods:Carrageenan-induced mouse paw edema model was used for induction of inflammation.Results:The results showed that intraplantar injection of carrageenan led to time-dependent development of peripheral inflammation, which resulted in a significant increase in the levels of tumor necrosis factor α (TNF-α) and interleukin 1 (IL-1) β, nitric oxide (NO) and prostaglandin E2 (PGE2) and also iNOS and COX-2 protein expression in inflamed paw. However, systemic administration of EA (1–30 mg/kg, intraperitoneal [i.p.]) could reduce edema in a dose-dependent fashion in inflamed rat paws with ED50 value 8.41 (5.26–14.76) mg/kg. It decreased the serum concentration of NO, PGE2, aspartate aminotransferase and alanine aminotransferase, and suppress the protein expression of iNOS, COX-2 enzymes, and attenuated the formation of PGE2, TNF-α and IL-1 β in inflamed paw tissue. We also demonstrated that EA significantly decreased the malondialdehyde (MDA) level in liver at 5 h after carrageenan injection. Moreover, histopathological studies indicated that EA significantly diminished migration of polymorphonuclear leukocytes into site of inflammation, as did indomethacin.Conclusions:Collectively, the anti-inflammatory mechanisms of EA might be related to the decrease in the level of MDA, iNOS, and COX-2 in the edema paw via the suppression of pro-inflammatory cytokines (TNFα, IL1 β), NO and PGE2 overproduction.

show abstract

“…Tail flick and hot plate test are used to explore those analgesic compounds that produce antinociceptive effect through centrally mediated mechanisms, while formalin test is used to elucidate those compounds that produce antinociceptive effects through both peripheral and central mechanisms. In another way, it can be implicit that both tail flick and hot plate tests are selectively used to screen the central analgesic agents (e.g., opiates) acting through opioid receptors [40,41].…”

Section: Discussionmentioning

confidence: 99%

Effects of Cinnamomum Zeylanicum Bark Extract on Nociception and Anxiety Like Behavior in Mice

Jain

Gupta

2019

Asian J Pharm Clin Res

View full text Add to dashboard Cite

Objectives: The aim of the study was to assess the effect of the extract of Cinnamomum zeylanicum (CZ) bark in the experimental models of pain and anxiety-like behavior in mice. Methods: The extract of CZ bark was administered at the doses of 100, 200, and 400 mg/kg, per orally (p.o) and morphine used as a positive control for pain models, was administered at the dose of 5 mg/kg, intraperitoneally (i.p.). Antinociceptive activity was evaluated using three experimental animal models of pain, namely, tail flick, hot plate, and formalin test. Elevated plus maze test was used to assess the effect on anxiety-like behavior. Rotarod apparatus and actophotometer were used to test muscle coordination and locomotor activity, respectively. Results: Administration of CZ bark extract in the dose of 200 and 400 mg/kg showed significantly increased in the tail-flick latency and latency to reaction time in hot plate test as compared to the control group. In the first phase (0–5 min) of the formalin test, a significant reduction in the pain response was found in CZ (200 and 400 mg/kg) and morphine-treated groups, however during the second phase (30–35 min) significant reduction in formalin-induced pain response was observed in 100, 200, and 400 mg/kg CZ extract-treated group when compared to control group. CZ extract administration at 200 and 400 mg/kg dose caused a significant increase in the percentage of time spent in open arms in the elevated plus maze as compared to the control group. Conclusion: Results suggest that CZ bark extract possesses the antinociceptive activity and modulates anxiety-like behavior.

show abstract

Sildenafil enhances the peripheral antinociceptive effect of ellagic acid in the rat formalin test

Cited by 8 publications

References 20 publications

Natural antioxidant flavonoids in formalin‐induced mice paw inflammation; inhibition of mitochondrial sorbitol dehydrogenase activity

Natural antioxidant flavonoids in formalin‐induced mice paw inflammation; inhibition of mitochondrial sorbitol dehydrogenase activity

A study of the mechanisms underlying the anti-inflammatory effect of ellagic acid in carrageenan-induced paw edema in rats

Effects of Cinnamomum Zeylanicum Bark Extract on Nociception and Anxiety Like Behavior in Mice

Contact Info

Product

Resources

About