A study of the mechanisms underlying the anti-inflammatory effect of ellagic acid in carrageenan-induced paw edema in rats

Mansouri, Mohammad Taghi; Hemmati, Ali Asghar; Naghizadeh, Bahareh; Mard, Seyyed Ali; Rezaie, Annahita; Ghorbanzadeh, Behnam

doi:10.4103/0253-7613.157127

Cited by 147 publications

(40 citation statements)

References 28 publications

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“…Although this effect was also observed in the kaolin inflammation model, the anti-inflammatory effect was not statistically significant in this case. This finding suggests that the glycerolic extract might be rich in active constituents able to interfere with the proinflammatory mechanism of carrageenan, which produces an increase in TNF-α, IL-1, nitric oxide, and PGE-2 levels [45]. Moreover, previous published studies highlighted that horse chestnut ethanolic extracts inhibit elastase and hyaluronidase, thus diminishing capillary permeability and edema [20].…”

Section: Discussionmentioning

confidence: 92%

Evaluation of Natural Extracts in Animal Models of Pain and Inflammation for a Potential Therapy of Hemorrhoidal Disease

et al. 2019

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The aim of this work was to assess the analgesic effect of three Vitis vinifera L. leaf extracts and the anti-inflammatory effect of three gels obtained from Aesculus hippocastanum L. seed extracts using animal models, as a preliminary study for the future development of topical preparations based on the combination of extracts with synergistic therapeutic effects on hemorrhoid disease. The analgesic effect was determined by means of the writhing test in mice. The anti-inflammatory effect was determined after administration of carrageenan or kaolin in the rat paw. Extraction using glycerol yielded the highest amounts of flavonoids for both V. vinifera leaves (37.27 ± 1.174 mg/L) and A. hippocastanum seeds (53.48 ± 0.212 mg/L). The highest total phenolic contents were registered for the V. vinifera 20% ethanolic extract (615.3 ± 34.44 mg/L) and for the A. hippocastanum glycerolic extract (247.8 ± 6.991 mg/L). The writhing test revealed that the V. vinifera ethanolic extract induced the most efficient analgesia (57.20%, p < 0.01), better than that induced by the positive control. In the carrageenan inflammation model, only the gel obtained from the A. hippocastanum glycerolic extract significantly reduced paw edema (17.27%, p < 0.05). An anti-inflammatory effect was also observed in the kaolin inflammation model but was not statistically significant (10.12%, p > 0.05). Our findings indicate that V. vinifera and A. hippocastanum extracts may have potential uses for the treatment of pain and inflammation associated with hemorrhoid disease.

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Section: Discussionmentioning

confidence: 92%

Evaluation of Natural Extracts in Animal Models of Pain and Inflammation for a Potential Therapy of Hemorrhoidal Disease

et al. 2019

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“…Moreover, the administrations of the CBE, CBW, and CBW-1 without CO application did not cause any inflammation or tissue damage in the rats. (Dey et al, 2016;Mansouri et al, 2015;Wang et al, 2009). Therefore, TNF-α, IL-6, and MPO are widely used as indices of tissue damage caused by various inflammatory factors.…”

Section: Re Sults and Discussionmentioning

confidence: 99%

Antihemorrhoidal activity of organic acids ofCapsella bursa‐pastorison croton oil‐induced hemorrhoid in rats

et al. 2020

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This study was aimed to investigate the antihemorrhoidal effects of ethanol (CBE) and water extracts (CBW) of Capsella bursa‐pastoris, an edible plant and a precipitant (CBW‐1) obtained from the CBW in croton oil (CO)‐induced hemorrhoid model in rats. CBW‐1 was contain three organic acids, citric acid (36.09%), malic acid (35.56%), and quinic acid (17.73%). Hemorrhoids were evaluated by histopathology of recto‐anal tissues and biochemical parameters in plasma and recto‐anal tissues of rats. CBW, CBE, and CBW‐1 significantly reduced hemorrhagic necrotic enteritis induced by CO. CO also increased the cytokines and lipid peroxidation (LPO) in serum, and myeloperoxidase (MPO) activity and LPO in recto‐anal tissues, and reduced the GSH, CAT, GPx, and SOD levels in serum and recto‐anal tissues. However, CBE, CBW, and CBW‐1 ameliorated the levels of the cytokines, LPO, MPO, and the antioxidants. Our results conclude that the curative effect of Capsella bursa‐pastoris is closely related with its organic acids constituents, citric, malic, and quinic acids. Practical applications The fresh leaves of Capsella bursa‐pastoris are edible, eaten raw or cooked, and also used in salad. It has a widespread traditional usage in the treatment of the hemorrhoids in the Anatolia and in the Middle East Countries. According to our literature survey, any scientific evidence has not been found in the literature that C. bursa‐pastoris could be used in the treatment of hemorrhoids. Therefore, in the current study, we aimed to investigate the antihemorrhoidal and antioxidant effects of ethanol and water extracts, and a precipitant (CBW‐1) obtained from the CBW of C. bursa‐pastoris in croton oil (CO)‐induced hemorrhoid model in rats. The current results showed that its water extract and CBW‐1 containing three organic acids, citric acid (36.09%), malic acid (35.56%), and quinic acid (17.73%) significantly reduced the hemorrhagic necrotic enteritis induced by CO ameliorating the levels of the cytokines, LPO, MPO, and the antioxidants. Our results conclude that the curative effect of C. bursa‐pastoris is closely related with its organic acids constituents, citric, malic, and quinic acids.

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“…TNF- α , IL-1 β , and IL-6 are known as the most representative proinflammatory cytokines. Specifically, TNF- α is able to initiate other inflammatory mediators and thus augments the responses to inflammatory stimuli [ 23 ]. IL-1 β has profound effects on inflammation and immunity and is responsible for cartilage destruction and bone resorption [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

“…Our results revealed that PAO significantly suppressed NO release and downregulated iNOS expression in paw tissues. Furthermore, Mansouri et al [ 23 ] revealed that NO also involved in catalyzing of COX-2 biosynthesis. The underlying mechanism possibly relates to lipid peroxidation initiated by peroxynitrite, which accounts for arachidonic acid liberation and COX-2 pathway activation [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Patchoulene Epoxide Isolated from Patchouli Oil Suppresses Acute Inflammation through Inhibition of NF-κB and Downregulation of COX-2/iNOS

Liang

Liu

et al. 2017

Mediators of Inflammation

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According to the GC-MS analysis, compositional variation was observed between samples of patchouli oil, of which an unknown compound identified as patchoulene epoxide (PAO) was found only in the long-stored oil, whose biological activity still remains unknown. Therefore, the present study aimed to evaluate the potential anti-inflammatory activity with three in vivo inflammatory models: xylene-induced ear edema, acetic acid-induced vascular permeability, and carrageenan-induced paw edema. Further investigation into its underlying mechanism on carrageenan-induced paw edema was conducted. Results demonstrated that PAO significantly inhibited the ear edema induced by xylene, lowered vascular permeability induced by acetic acid and decreased the paw edema induced by carrageenan. Moreover, PAO markedly decreased levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), prostaglandin E2 (PGE2), and nitric oxide (NO), but increased levels of interleukin-4 (IL-4) and interleukin-10 (IL-10). PAO was also shown to significantly downregulate the protein and mRNA expressions of cyclooxygenase-2 (COX-2) and inducible nitric-oxide synthase (iNOS). Western blot analysis revealed that PAO remarkably inhibited p50 and p65 translocation from the cytosol to the nucleus by suppressing IKKβ and IκBα phosphorylation. In conclusion, PAO exhibited potent anti-inflammatory activity probably by suppressing the activation of iNOS, COX-2 and NF-κB signaling pathways.

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A study of the mechanisms underlying the anti-inflammatory effect of ellagic acid in carrageenan-induced paw edema in rats

Cited by 147 publications

References 28 publications

Evaluation of Natural Extracts in Animal Models of Pain and Inflammation for a Potential Therapy of Hemorrhoidal Disease

Evaluation of Natural Extracts in Animal Models of Pain and Inflammation for a Potential Therapy of Hemorrhoidal Disease

Antihemorrhoidal activity of organic acids ofCapsella bursa‐pastorison croton oil‐induced hemorrhoid in rats

Patchoulene Epoxide Isolated from Patchouli Oil Suppresses Acute Inflammation through Inhibition of NF-κB and Downregulation of COX-2/iNOS

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