Significant improvement of sperm DNA quality after microsurgical repair of varicocele

Li, Fuping; Yamaguchi, Kensei; Okada, Keisuke; Matsushita, Kei; Ando, Motomi; Chiba, Koji; Yue, Huanxun; Fujisawa, Masato

doi:10.3109/19396368.2012.692431

Cited by 66 publications

(44 citation statements)

References 21 publications

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“…As expected and in agreement with background literature [42][43][44], the mean percentage of sperm DNA fragmentation was higher in individuals with varicocele compared to fertile men. In addition, Park et al [45] demonstrated a significant correlation between the expression of PLCζ and the DNA oxidation status assessed using 8-hydroxy-2-deoxyguanosine in human spermatozoa [45].…”

Section: Discussionsupporting

confidence: 92%

An association between sperm PLCζ levels and varicocele?

Janghorban-Laricheh

Ghazavi-Khorasgani

Tavalaee

et al. 2016

J Assist Reprod Genet

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Purpose We aimed to compare the expression of phospholipase C ζ (PLCζ), as one of the main sperm factors involved in oocyte activation, at both RNA and protein levels in fertile men and those with varicocele. Methods This study included 35 individuals with male factor infertility presenting primary infertility with grade II and III unilateral varicocele and 20 fertile men without varicocele. Semen parameters were assessed according to WHO 2010. Sperm DNA fragmentation, relative expression of PLCζ at messenger RNA, and protein levels were evaluated by sperm chromatin structure assay (SCSA), real-time PCR, and Western blot analysis, respectively. Results The results of this study reveal that the mean relative expression of PLCζ was significantly lower in individuals with varicocele compared to fertile men at both transcription and translation levels. In addition, the percentage of DNA fragmentation was significantly higher in infertile men with varicocele compared to fertile men. Conclusions The findings of the present study illustrate that one of the etiologies of reduced fertility associated with varicocele is the low expression of PLCζ. This effect could subsequently reduce the sperm ability to induce oocyte activation. Therefore, these results hold promise to modify our understanding of reproductive physiology of varicocele state.

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Section: Discussionsupporting

confidence: 92%

An association between sperm PLCζ levels and varicocele?

Janghorban-Laricheh

Ghazavi-Khorasgani

Tavalaee

et al. 2016

J Assist Reprod Genet

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“…Several studies have indicated that high levels of ROS in seminal fluid increase the risk of male infertility including varicocele (9,10). Elevated levels of ROS in semen samples of varicocele infertile men were observed, which were associated with an increased DNA fragmentation index (9,11,12). These findings suggest that the imbalance between oxidant capacity and the natural antioxidant defense system could play a role in varicocele-associated infertility.…”

Section: Introductionmentioning

confidence: 94%

Association of Seminal Plasma Total Antioxidant Capacity and Malondialdehyde Levels With Sperm Parameters in Infertile Men With Varicocele

Salimi¹,

Fazeli²,

Khosravi³

et al. 2016

Avicenna J Med Biochem

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“…[2][3][4] While varicoceles are the most easily treatable cause of male infertility, these vascular malformations are associated with a progressive worsening of testicular function if left untreated. [5][6][7] Varicocele repair improves semen quality and sperm DNA integrity, 8 increases clinical pregnancy and live birth rates during in-vitro fertilization (IVF) via intra-cytoplasmic sperm injection (ICSI) 9 and potentially recovers semen parameters in men with nonobstructive azoospermia. 10,11 While the effects of varicocele repair are well-established, the method of correction that is most cost-effective, is controversial.…”

Section: Introductionmentioning

confidence: 99%

Cost-effectiveness analysis reveals microsurgical varicocele repair is superior to percutaneous embolization in the treatment of male infertility

Kovac

Fantus

Lipshultz

et al. 2014

CUAJ

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Introduction: Varicoceles are a common cause of male infertility; repair can be accomplished using either surgical or radiological means. We compare the cost-effectiveness of the gold standard, the microsurgical varicocele repair (MV), to the options of a nonmicrosurgical approach (NMV) and percutaneous embolization (PE) to manage varicocele-associated infertility. Methods: A Markov decision-analysis model was developed to estimate costs and pregnancy rates. Within the model, recurrences following MV and NMV were re-treated with PE and recurrences following PE were treated with repeat PE, MV or NMV. Pregnancy and recurrence rates were based on the literature, while costs were obtained from institutional and government supplied data. Univariate and probabilistic sensitivity-analyses were performed to determine the effects of the various parameters on model outcomes. Results: Primary treatment with MV was the most cost-effective strategy at $5402 CAD (Canadian)/pregnancy. Primary treatment with NMV was the least costly approach, but it also yielded the fewest pregnancies. Primary treatment with PE was the least cost-effective strategy costing about $7300 CAD/pregnancy. Probabilistic sensitivity analysis reinforced MV as the most cost-effective strategy at a willingness-to-pay threshold of >$4100 CAD/pregnancy. Conclusions: MV yielded the most pregnancies at acceptable levels of incremental costs. As such, it is the preferred primary treatment strategy for varicocele-associated infertility. Treatment with PE was the least cost-effective approach and, as such, is best used only in cases of surgical failure.

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Significant improvement of sperm DNA quality after microsurgical repair of varicocele

Cited by 66 publications

References 21 publications

An association between sperm PLCζ levels and varicocele?

An association between sperm PLCζ levels and varicocele?

Association of Seminal Plasma Total Antioxidant Capacity and Malondialdehyde Levels With Sperm Parameters in Infertile Men With Varicocele

Cost-effectiveness analysis reveals microsurgical varicocele repair is superior to percutaneous embolization in the treatment of male infertility

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