Significant improvement of Geobacillus thermoleovorans CCR11 thermoalkalophilic lipase production using Response Surface Methodology

Sánchez-Otero, María G.; Ruiz-López, I.I.; Ávila-Nieto, David Eduardo; Oliart-Ros, Rosa María

doi:10.1016/j.nbt.2011.01.010

Cited by 11 publications

(10 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The maximum lipase activity has been achieved when inoculums size and incubation time near their zero levels. The nonlinear relationship of fermentation process variables, namely, inoculum concentration and temperature on lipase yield from Geobacillus thermoleovorans CCR11 has been also found through the RSM approach [19]. Significant and combined effects of polydimethylsiloxane (PDMS) and oxygen volumetric mass transfer coefficient through RSM have been acknowledged by Rech et al [20] for lipase production by Staphylococcus warneri EX17.…”

Section: Resultsmentioning

confidence: 99%

Modelling and Optimization Studies on a Novel Lipase Production by Staphylococcus arlettae through Submerged Fermentation

Chauhan

Garlapati

2013

Enzyme Research

View full text Add to dashboard Cite

Microbial enzymes from extremophilic regions such as hot spring serve as an important source of various stable and valuable industrial enzymes. The present paper encompasses the modeling and optimization approach for production of halophilic, solvent, tolerant, and alkaline lipase from Staphylococcus arlettae through response surface methodology integrated nature inspired genetic algorithm. Response surface model based on central composite design has been developed by considering the individual and interaction effects of fermentation conditions on lipase production through submerged fermentation. The validated input space of response surface model (with R 2 value of 96.6%) has been utilized for optimization through genetic algorithm. An optimum lipase yield of 6.5 U/mL has been obtained using binary coded genetic algorithm predicted conditions of 9.39% inoculum with the oil concentration of 10.285% in 2.99 hrs using pH of 7.32 at 38.8°C. This outcome could contribute to introducing this extremophilic lipase (halophilic, solvent, and tolerant) to industrial biotechnology sector and will be a probable choice for different food, detergent, chemical, and pharmaceutical industries. The present work also demonstrated the feasibility of statistical design tools integration with computational tools for optimization of fermentation conditions for maximum lipase production.

show abstract

Section: Resultsmentioning

confidence: 99%

Modelling and Optimization Studies on a Novel Lipase Production by Staphylococcus arlettae through Submerged Fermentation

Chauhan

Garlapati

2013

Enzyme Research

View full text Add to dashboard Cite

show abstract

“…Many thermostable lipase producing microbial sources are available as wild and recombinant strains. In this relevant, the members of the genus Geobacillus are able to produce extracellular lipase enzymes, which have wide applications in biotechnology . Extracellular lipase enzyme production from this genus was shown high thermostable property, substrate specificity, resistant to mild alkaline condition, solvent degradation and optimization are less expensive .…”

Section: Discussionmentioning

confidence: 99%

Thermostable lipase fromGeobacillussp. Iso5: Bioseparation, characterization and native structural studies

Mahadevan

Neelagund

2013

J. Basic Microbiol.

View full text Add to dashboard Cite

The extracellular thermoalkaline lipase from Geobacillus sp. Iso5 was purified to homogeneity by ultrafiltration, 6% cross-linked agarose and Phenyl spehrose HIC column chromatography. The final purified lipase resulted in 8.7-fold with 6.2% yield. The relative molecular weight of the enzyme was determined to be a monomer of 47 kDa by SDS-PAGE and MALDI-TOF MS/MS spectroscopy. The purified enzyme exhibit optimum activity at 70 °C and pH 8.0. The enzyme retained above 90% activity at temperatures of 70 °C and about 35% activity at 85 °C for 2 h. However, the stability of the enzyme decreased at the temperature over 90 °C. The enzyme activity was promoted in the presence of Ca(2+) and Mg(2+) and strongly inhibited by HgCl2 , PMSF, DTT, K(+) , Co(2+) , and Zn (2+) . EDTA did not affect the enzyme activity. The secondary structure of purified lipase contains 36% α-helix and 64% β-sheet which was determined by Circular dichromism, FTIR, and Raman Spectroscopy.

show abstract

“…However, statistical design methods using computer software have been recently utilized more often to perform optimization procedures [3,24,25]. Lipase activity from different strains is unique, depending upon the microorganisms, culture conditions and nutrients available.…”

Section: Discussionmentioning

confidence: 99%

“…The two models lack of fit and curvature were not significant, meaning that the models fit the data appropriately. The final equation was as follows: Y = 82.09 + 9.87 X 1 + 9.78 X 2 + 3.23 X 4 − 8.96 X 8 (3) where Y is the response (lipase activity) and X 1 , X 2 , X 4 and X 8 are coded values of the significant factors respectively. The multiple correlation coefficient (R 2 ) of the first-order model was 0.86, implying that 86% of data variation can be determined by the model.…”

Section: Plackett-burman Designmentioning

confidence: 99%

“…Due to their distinctive biotechnological versatility and ability to catalyze a wide range of bioconversion reactions, such as the synthesis of pharmaceutical intermediates, agrochemicals and flavor compounds they have attracted attention recently [2,3]. Microbial lipases are more stable than those obtained from plants and animals, while lipases produced by Bacillus, Acinetobacter, Staphylococcus and Pseudomonas have shown high stereo and regio-selectivities, making them important tools in biocatalysis [3,4].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Significant enhancement of Pseudomonas aeruginosa FW_SH-1 lipase production using response surface methodology and analysis of its hydrolysis capability

Ali

Mbadinga

Liu

et al. 2015

Journal of the Taiwan Institute of Chemical Engineers

View full text Add to dashboard Cite

Significant improvement of Geobacillus thermoleovorans CCR11 thermoalkalophilic lipase production using Response Surface Methodology

Cited by 11 publications

References 31 publications

Modelling and Optimization Studies on a Novel Lipase Production by Staphylococcus arlettae through Submerged Fermentation

Modelling and Optimization Studies on a Novel Lipase Production by Staphylococcus arlettae through Submerged Fermentation

Thermostable lipase fromGeobacillussp. Iso5: Bioseparation, characterization and native structural studies

Significant enhancement of Pseudomonas aeruginosa FW_SH-1 lipase production using response surface methodology and analysis of its hydrolysis capability

Contact Info

Product

Resources

About