Significant enhancement of Pseudomonas aeruginosa FW_SH-1 lipase production using response surface methodology and analysis of its hydrolysis capability

Ali, Chaudhry Haider; Mbadinga, Serge Maurice; Liu, Jinfeng; Yang, Shi‐Zhong; Gu, Ji‐Dong; Mu, Bo‐Zhong

doi:10.1016/j.jtice.2015.02.001

Cited by 17 publications

(3 citation statements)

References 33 publications

(49 reference statements)

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“…Haider Ali et al (2015) reported the significant negative effect of NaCl on lipase production by Pseudomonas aeruginosa FW_SH-1. However, Sathishkumar et al (2015) reported the positive effect of NaCl on lipase production by Halobacillus trueperi RSK CAS9.…”

Section: Medium Optimizationmentioning

confidence: 99%

“…Investigation on the factors influencing the production of biomolecules is essential in many bioprocess development procedures (Forootanfar et al 2015;Moshafi et al 2011). Generally, a higher productivity could be achieved by culture medium optimization (Haider Ali et al 2015;Hasan-Beikdashti et al 2012;Riyadi et al 2017). The statistical optimization of processes has advantages compared to the traditional method of varying one factor at a time (Colla et al 2016;Faisal et al 2014) such as the lower number of experiments and the possibility of evaluating the interaction effects between factors.…”

Section: Introductionmentioning

confidence: 99%

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Statistical optimization of cultural medium composition of thermoalkalophilic lipase produced by a chemically induced mutant strain of Bacillus atrophaeus FSHM2

et al. 2019

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Extremophilic microbial derived lipases have been widely applied in different biotechnological processes due to their resistance to harsh conditions such as high salt concentration, elevated temperature, and extreme acidic or alkaline pH. The present study was designed to overproduce the halophilic, thermoalkalophilic lipase of Bacillus atrophaeus FSHM2 through chemically induced random mutagenesis and optimization of cultural medium components assisted by statistical experimental design. At first, improvement of lipase production ability of B. atrophaeus FSHM2 was performed through exposure of the wild bacterial strain to ethidium bromide for 5-90 min to obtain a suitable mutant of lipase producer (designated as EB-5, 4301.1 U/l). Afterwards, Plackett-Burman experimental design augmented to D-optimal design was employed to optimize medium components (olive oil, maltose, glucose, sucrose, tryptone, urea, (NH 4 ) 2 SO 4 , NaCl, CaCl 2 , and ZnSO 4 ) for lipase production by the EB-5 mutant. A maximum lipase production of 14,824.3 U/l was predicted in the optimum medium containing 5% of olive oil, 0.5% of glucose, 0.5% of sucrose, 2% of maltose, 2.5 g/l of yeast extract, 1.75 g/l of urea, 1.75 g/l of (NH 4 ) 2 SO 4 , 2.5 g/l of tryptone, 2 g/l of NaCl, 1 g/l of CaCl 2 , and 1 g/l of ZnSO 4 . A mean value of 14,773 ± 576.9 U/l of lipase was acquired from real experiments.

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Section: Medium Optimizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Statistical optimization of cultural medium composition of thermoalkalophilic lipase produced by a chemically induced mutant strain of Bacillus atrophaeus FSHM2

et al. 2019

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“…Moreover, validation experiments showed a good correlation coefficient of 0.994 and very low p value, i.e., the quadratic polynomial model was highly significant and indicated high correlation between the predicted and observed values of biodiesel produced. Ali et al (2015) reported the quadratic response models to be successfully applied for the production of biodiesel using onsite lipase-mediated reaction with central composite design. Similar studies were conducted by Chen et al (2002) and Shieh et al (2003).…”

Section: Statistical Optimizationmentioning

confidence: 99%

Optimization of parameters for the production of biodiesel from rubber seed oil using onsite lipase by response surface methodology

et al. 2018

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In the present study, we demonstrate the properties of rubber seed oil (RSO) and its potential application for producing biodiesel by lipase-catalyzed transesterification. Rubber seed contains 39.45% of oil on a dry weight basis. Lipase was secreted from novel bacterial isolate of Pseudomonas aeruginosa strain BUP2. Response Surface Methodology (RSM) cum Box-Behnken Design (BBD) was employed to optimize the combined effect of different independent parameters namely oil-methanol ratio, enzyme unit, reaction temperature and reaction time. Biodiesel yield of 99.52% was obtained in the validation experiments at the optimal level of lipase (750 U), methanol ratio (1:10), temperature (45 °C) and time (4 h). The fuel properties of biodiesel obtained under the validation condition met the specifications as mentioned in ASTM D6751 and EN 14214 standards. Biodiesel aliquots were characterized using thin-layer chromatography (TLC), gas chromatography (GC), fourier transform infra-red spectroscopy (FTIR) and elemental analysis. The present study demonstrates an important application of a potential substitute for fossil fuel from raw feedstocks with high economic value.

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Optimization and analysis of reaction injection molding of polydicyclopentadiene using response surface methodology

Kim

Son

Lee

et al. 2017

Korean J. Chem. Eng.

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Significant enhancement of Pseudomonas aeruginosa FW_SH-1 lipase production using response surface methodology and analysis of its hydrolysis capability

Cited by 17 publications

References 33 publications

Statistical optimization of cultural medium composition of thermoalkalophilic lipase produced by a chemically induced mutant strain of Bacillus atrophaeus FSHM2

Statistical optimization of cultural medium composition of thermoalkalophilic lipase produced by a chemically induced mutant strain of Bacillus atrophaeus FSHM2

Optimization of parameters for the production of biodiesel from rubber seed oil using onsite lipase by response surface methodology

Optimization and analysis of reaction injection molding of polydicyclopentadiene using response surface methodology

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