Optimization of parameters for the production of biodiesel from rubber seed oil using onsite lipase by response surface methodology

Abstract

In the present study, we demonstrate the properties of rubber seed oil (RSO) and its potential application for producing biodiesel by lipase-catalyzed transesterification. Rubber seed contains 39.45% of oil on a dry weight basis. Lipase was secreted from novel bacterial isolate of Pseudomonas aeruginosa strain BUP2. Response Surface Methodology (RSM) cum Box-Behnken Design (BBD) was employed to optimize the combined effect of different independent parameters namely oil-methanol ratio, enzyme unit, reaction temperature and reaction time. Biodiesel yield of 99.52% was obtained in the validation experiments at the optimal level of lipase (750 U), methanol ratio (1:10), temperature (45 °C) and time (4 h). The fuel properties of biodiesel obtained under the validation condition met the specifications as mentioned in ASTM D6751 and EN 14214 standards. Biodiesel aliquots were characterized using thin-layer chromatography (TLC), gas chromatography (GC), fourier transform infra-red spectroscopy (FTIR) and elemental analysis. The present study demonstrates an important application of a potential substitute for fossil fuel from raw feedstocks with high economic value.

Application of artificial neural networks for enhancing Aspergillus flavipes lipase synthesis for green biodiesel production

“…The fungal lipase obtained based on the previous optimum conditions - inferred by the ANN model - was incorporated in the lipase biosynthesis trial. The reaction was carried out according to Panichikkal et al [ 23 ] with some modifications. A mixture of corn-oil, and methanol at a molar ratio of 1: 9 was added to a 250-ml conical flask.…”

Fabrication and Optimization of Nanocatalyst for Biodiesel Production: An Overview

“…These waste cooking oils containing free fatty acids are ultimately reduced by using pre-treatment processes and alkali based trans-esterification process as high fatty acid content leads to soap formation. However, the pre-treatment process is mainly affected by various reaction parameters (Faisal et al, 2018). Biodiesel production mainly occurs by esterification and trans-esterification of free fatty acids and triglycerides, as the amount of free fatty acid corresponds to its yield.…”

Application of Response Surface Methodology (RSM) to Study Transesterification of Palm Oil in the Presence of Zeolite-A as Catalyst

“…However, since glycerides are the prominent components of vegetable oil, transesterification is the main issue in biodiesel production. Several examples of vegetable oils that have been reported in the literature are waste cooking oil [1][2], coconut oil [3], Ricinus communis oil [4], rubber seed oil [5] [6], and soybean oil [7]. Concerning this reaction, biodiesel is also known as a mixture of fatty acid methyl esters (FAMEs).…”

“…To overcome these deficiencies, the use of multi-variable statistical techniques is now increasingly explored; one of them is known as response surface methodology (RSM) [7] [20]. RSM is a statistical technique that uses multiple regression and correlation analysis as a tool to assess the effect of two or more independent variables on the dependent variable, with the aid of analysis of variance (ANOVA) [6] [10] [13][21]. This statistical method has been applied for the optimization of transesterification of different vegetable oils such as papaya oil [22], Kusum oil [23], and low-grade cooking oil [24].…”