Significant difference in response of malignant tumor cells of individual patients to photodynamic treatment as revealed by digital holographic microscopy

Machine Learning Assisted Classification of Cell Lines and Cell States on Quantitative Phase Images

“…Thus trained classification algorithm based on the SVM classifier with quadratic kernel functions operating with the set of 10 cell parameters was applied to analysis of the dynamics of cell response to PDTr with different doses. As we have previously shown [36,42], different doses of PDTr, provided by variation of either irradiation power density or irradiation duration or both can cause different pathways of cell death and different death dynamics. In experiments we varied the power density keeping constant the irradiation duration.…”

“…The obtained optical parameters of cells depend on both morphological characteristics and refractive index distributions, related to dry mass density in different cellular compartments. Analysis of these parameters allows for assessment of cell life cycle and growth [32], volumetry and refractometry [33], treatment efficacy of cancer cells [34,35], quantitative estimation of the dynamics of morphological changes [36,37], etc.…”

Analysis of cellular response to photodynamic treatment with Radachlorin photosensitizer by means of quantitative phase microscopy using highly coherent and partially coherent light sources

“…Similar decrease of this parameter along with the decrease of dry mass was reported for other types of living cells as well. 42,43 On the other hand, apoptosis of HeLa cells resulted in the increase of average phase shift detected by off-axis DHM, TIE and SLIM.…”

Recording of Long Low-Amplitude Bulk Elastic Waves in Transparent Solid Waveguides by Digital and Classical Holography

“…The development of high-speed digital cameras equipped with fast, global shutters allowed eliminating the need to probe rapid dynamic processes using pulsed lasers [26]. At the same time, despite much more frequent use of digital holography, especially in the field of microscopy and biology [27][28][29][30][31], holographic interferometry with traditional recording on holographic plates and films is still in demand in a number of applications [32][33][34][35][36]. Among these applications is the recording of elastic waves of different types in solids, in particular, shock and strain solitary waves (solitons) in solid waveguides.…”