Significant boost in xylose yield and enhanced economic value with one-pot process using deep eutectic solvent for the pretreatment and saccharification of rice straw

Huang, Zijian; Feng, Guo-Jian; Lin, Kai-Peng; Pu, Fu-Long; Tan, Yan-Ming; Tu, Wei-Chien; Han, Yuping; Hou, Xue-Dan; Zhang, Hongmei; Zhang, Yi

doi:10.1016/j.indcrop.2020.112515

Cited by 36 publications

(13 citation statements)

References 24 publications

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“…Recently, a new class of catalysts called as the deep eutectic solvents (DESs) that consist of a eutectic-mix of Lewis or Brønsted acids and bases with atleast one hydrogen bond donor (HBD) and an acceptor (HBA) were found to be equally promising for delignification 22 , 23 . Moreover, DESs are effective, cheaper, biodegradable and recyclable solvents 24 ; albeit being slow-acting (12–24 h) 25 , 26 and may show some deterioration over time 27 , 28 . Overall, the choice of the pretreatment influences the nature of cellulose-rich solids and its amenability to hydrolysis by cellulases.…”

Section: Introductionmentioning

confidence: 99%

Generation of highly amenable cellulose-Iβ via selective delignification of rice straw using a reusable cyclic ether-assisted deep eutectic solvent system

Chiranjeevi

Balasubramaniam

Velankar

2021

Sci Rep

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Cellulolytic enzymes can readily access the cellulosic component of lignocellulosic biomass after the removal of lignin during biomass pretreatment. The enzymatic hydrolysis of cellulose is necessary for generating monomeric sugars, which are then fermented into ethanol. In our study, a combination of a deep eutectic (DE) mixture (of 2-aminoethanol and tetra-n-butyl ammonium bromide) and a cyclic ether (tetrahydrofuran) was used for selective delignification of rice straw (RS) under mild conditions (100 °C). Pretreatment with DE-THF solvent system caused ~ 46% delignification whereas cellulose (~ 91%) and hemicellulose (~ 67%) recoveries remained higher. The new solvent system could be reused upto 10 subsequent cycles with the same effectivity. Interestingly, the DE-THF pretreated cellulose showed remarkable enzymatic hydrolysability, despite an increase in its crystallinity to 72.3%. Contrary to conventional pretreatments, we report for the first time that the enzymatic hydrolysis of pretreated cellulose is enhanced by the removal of lignin during DE-THF pretreatment, notwithstanding an increase in its crystallinity. The current study paves way for the development of newer strategies for biomass depolymerization with DES based solvents.

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Section: Introductionmentioning

confidence: 99%

Generation of highly amenable cellulose-Iβ via selective delignification of rice straw using a reusable cyclic ether-assisted deep eutectic solvent system

Chiranjeevi

Balasubramaniam

Velankar

2021

Sci Rep

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“…Traditional procedures for the exploitation of the solid and liquid phases require a prior separation of the fractions, with a resulting consumption of water and energy to wash the pretreated biomass [27]. Regarding this, a strategy based on one-pot reactions would entail both environmental and economic advantages, avoiding the isolation or purification of intermediate components [28,29].…”

Section: Introductionmentioning

confidence: 99%

A Whole-Slurry Fermentation Approach to High-Solid Loading for Bioethanol Production from Corn Stover

Río¹,

Gullón²,

Rebelo³

et al. 2020

Agronomy

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Corn stover is the most produced byproduct from maize worldwide. Since it is generated as a residue from maize harvesting, it is an inexpensive and interesting crop residue to be used as a feedstock. An ecologically friendly pretreatment such as autohydrolysis was selected for the manufacture of second-generation bioethanol from corn stover via whole-slurry fermentation at high-solid loadings. Temperatures from 200 to 240 °C were set for the autohydrolysis process, and the solid and liquid phases were analyzed. Additionally, the enzymatic susceptibility of the solid phases was assessed to test the suitability of the pretreatment. Afterward, the production of bioethanol from autohydrolyzed corn stover was carried out, mixing the solid with different percentages of the autohydrolysis liquor (25%, 50%, 75%, and 100%) and water (0% of liquor), from a total whole slurry fermentation (saving energy and water in the liquid–solid separation and subsequent washing of the solid phase) to employing water as only liquid medium. In spite of the challenging scenario of using the liquor fraction as liquid phase in the fermentation, values between 32.2 and 41.9 g ethanol/L and ethanol conversions up to 80% were achieved. This work exhibits the feasibility of corn stover for the production of bioethanol via a whole-slurry fermentation process.

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“…Among the enzymes commonly required to degrade the plant matrix and used in both the biorefinery process and active molecule extraction [ 25 , 54 , 55 , 66 , 68 , 69 , 70 , 71 , 72 , 73 ], cellulase, pectinase, and laccase were screened to formulate an enzymatic cocktail. The variability of extraction yield explained by the factor pH according to the Taguchi analysis ( Figure A2 ) suggested better activity of the enzymes at pH range from 4 to 5 ( Figure A2 ).…”

Section: Discussionmentioning

confidence: 99%

“…In comparison to the formulated enzyme mixtures, the two commercial cocktails were more efficient for the recovery of phenolic and flavonoid content, and the highest TPC and TFC were obtained with Ultraflo© compared to Viscozyme© ( Figure 5 , Figure 6 and Figure 7 ). Ultraflo© and Viscozyme© enhance the TPC in Opuntia humifusa hydrolysate [ 72 ], and the Viscozyme© efficiency was confirmed for the recovery of specific flavonoids from Opuntia ficus-indica [ 67 ], which could be attributed to the presence of xylanases in both cocktails, which allows for a better degradation of the hemicellulose of A. lechuguilla composed at 63% of xylose [ 21 ]. Thus, xylanase should be considered in future mix formulations, such as those proposed by Wang et al [ 69 ].…”

Section: Discussionmentioning

confidence: 99%

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Early Optimization Stages of Agave lechuguilla Bagasse Processing toward Biorefinement: Drying Procedure and Enzymatic Hydrolysis for Flavonoid Extraction

et al. 2021

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Agave lechuguilla agro-waste is a promising renewable material for biorefining purposes. The procurement of added-value co-products, such as bioactive phytochemicals, is required to improve bioprocesses and promote the bio-based economy of the productive areas of Mexico. In this study, we aimed to evaluate the effect of post-harvest management and enzymatic pretreatment as the first stages of the A. lechuguilla valorization process. Four drying methods were compared, and enzymatic hydrolysis was optimized to obtain a flavonoid-enriched extract applying ultrasound-assisted extraction. In both experiments, the total phenolic (TPC) and flavonoid (TFC) contents, HPLC-UV flavonoid profiles, and radical scavenging capacity (DPPH) were considered as response variables. The results demonstrated that light exposure during the drying process particularly affected the flavonoid content, whereas oven-dehydration at 40 °C in the dark preserved the flavonoid diversity and antioxidant functionality of the extracts. Flavonoid glycoside recovery, particularly anthocyanidins, was 1.5–1.4-fold enhanced by enzymatic hydrolysis using the commercial mix Ultraflo© under optimized conditions (pH 4, 40 °C, 180 rpm, and 2.5 h) compared to the unpretreated biomass. The extraction of flavonoids from A. lechuguilla bagasse can be carried out using a scalable drying method and enzymatic pretreatment. This study confirmed the potential of this agro-waste as a source of marketable natural products.

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Significant boost in xylose yield and enhanced economic value with one-pot process using deep eutectic solvent for the pretreatment and saccharification of rice straw

Cited by 36 publications

References 24 publications

Generation of highly amenable cellulose-Iβ via selective delignification of rice straw using a reusable cyclic ether-assisted deep eutectic solvent system

Generation of highly amenable cellulose-Iβ via selective delignification of rice straw using a reusable cyclic ether-assisted deep eutectic solvent system

A Whole-Slurry Fermentation Approach to High-Solid Loading for Bioethanol Production from Corn Stover

Early Optimization Stages of Agave lechuguilla Bagasse Processing toward Biorefinement: Drying Procedure and Enzymatic Hydrolysis for Flavonoid Extraction

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