Significant associations of the mitochondrial transcription factor A promoter polymorphisms with marbling and subcutaneous fat depth in Wagyu x Limousin F2 crosses

Jiang, Zhihua; Kunej, Tanja; Michal, Jennifer J.; Gaskins, C. T.; Reeves, Jerry J.; Busboom, J. R.; Dovč, Peter; Wright, Raymond W.

doi:10.1016/j.bbrc.2005.06.120

Cited by 50 publications

(71 citation statements)

References 21 publications

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“…The SNP having the strongest association with fertility traits was in the gene transcription factor A, mitochondrial (TFAM). TFAM is an autosomal mitochondrial gene that encodes a histone-like protein essential for the transcription and replication of mitochondrial DNA (Jiang et al 2005). Cows that were GG homozygotes for the SNP TFAM3 not only produced less milk than the AG heterozygotes, but they also had worse fertility, being less likely to conceive and having a 24 day longer calving-to-calving interval (Clempson et al 2011a).…”

Section: Mitochondrial Genesmentioning

confidence: 99%

Associations between lipid metabolism and fertility in the dairy cow

Wathes

Clempson

Pollott

2013

Reprod. Fertil. Dev.

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Abstract. Dairy cows mobilise body tissues to support milk production and, because glucose supplies are limited, lipids are used preferentially for energy production. Lipogenic activity is switched off and lipolytic mechanisms in adipose tissue increase through changes in the expression of several key enzymes. This results in a loss of body condition, together with high circulating concentrations of non-esterified fatty acids. Changes in the synthesis, secretion and signalling pathways of somatotrophic hormones (insulin, growth hormone, insulin-like growth factor 1) and adipokines (e.g. leptin) are central to the regulation of these processes. A high reliance on fatty acids as an energy source in the peripartum period causes oxidative damage to mitochondria in metabolically active tissues, including the liver and reproductive tract. The expression of genes involved in insulin resistance (PDK4, AHSG) is increased, together with expression of TIEG1, a transcription factor that can induce apoptosis via the mitochondrial pathway. Polymorphisms in TFAM and UCP2, two autosomal mitochondrial genes, have been associated with longevity in dairy cows. Polymorphisms in many other genes that affect lipid metabolism also show some associations with fertility traits. These include DGAT1, SCD1, DECR1, CRH, CBFA2T1, GH, LEP and NPY. Excess lipid accumulation in oocytes and the regenerating endometrium reduces fertility via reductions in embryo survival and increased inflammatory changes, respectively.

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Section: Mitochondrial Genesmentioning

confidence: 99%

Associations between lipid metabolism and fertility in the dairy cow

Wathes

Clempson

Pollott

2013

Reprod. Fertil. Dev.

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“…They include the genes encoding m-calpaïn (Page et al, 2002;White et al, 2005) and lysyl oxidase and calpastatin (Barendse, 2001;Drinkwater et al, 2006) for tenderness. They also include the genes encoding leptin (Buchanan et al, 2002), DGAT1 (Thaller et al, 2003), TG (Barendse et al, 2004), RORC (Barendse, 2003), GH1 (Schlee et al, 1994), SCD, mitochondria (Mannen et al, 1998;2003), mitochondrial transcription factor A (Jiang et al, 2005) and FABP4 (Michal et al, 2006) for marbling. Summaries of the tests have been published (e.g.…”

Section: Bovine Genome Sequencing and Applications For Breedingmentioning

confidence: 99%

Recent advances in cattle functional genomics and their application to beef quality

Hocquette

Lehnert

Barendse

et al. 2007

Animal

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The advent of high-throughput DNA sequencing techniques, array technology and protein analysis has increased the efficiency of research in bovine muscle physiology, with the ultimate objective of improving beef quality either by breeding or rearing factors. For genetic purposes, polymorphisms in some key genes have been reported for their association with beef quality traits. The sequencing of the bovine genome has dramatically increased the number of available gene polymorphisms. The association of these new polymorphisms with the variability in beef quality (e.g. tenderness, marbling) for different breeds in different rearing systems will be a very important issue. For rearing purposes, global gene expression profiling at the mRNA or protein level has already shown that previously unsuspected genes may be associated either with muscle development or growth, and may lead to the development of new molecular indicators of tenderness or marbling. Some of these genes are specifically regulated by genetic and nutritional factors or differ between different beef cuts. In recognition of the potential economic benefits of genomics, public institutions in association with the beef industry are developing livestock genomics projects around the world. From the scientific, technical and economical points of view, genomics is thus reshaping research on beef quality.

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“…Molecular characterization of the porcine TFAM gene: Comparative annotation: The comparative approach for annotation of the porcine TFAM gene was described previously [7] . Basically, this annotation process consists of three steps.…”

Section: Methodsmentioning

confidence: 99%

“…For example, mice heterozygous for a targeted mutation of the Tfam gene exhibited reduced mtDNA copy number, while homozygous knockout embryos exhibited severe mtDNA loss and embryonic lethality, probably due to abolished transcription dependent priming of mtDNA replication [2,3] . In mammals, the TFAM gene has already been isolated and characterized in human [4] , mouse [5] , rat [6] , cattle [7] and silvered leaf-monkey [8] . The TFAM gene sequences for orangutan (CR857400), chimpanzee (XM_521481) and dog (XM_546107) were also deposited in the GenBank database (http://www.ncbi.nlm.nih.gov/).…”

Section: Introductionmentioning

confidence: 99%

The Porcine Mitochondrial Transcription Factor a Gene: Molecular Characterization, Radiation Hybrid Mapping and Genetic Diversity among 12 Pig Breeds

Kunej¹,

Wu²,

Michal³

et al. 2009

American J. of Animal and Veterinary Sciences

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Problem statement: Mitochondrial transcription factor A (TFAM) is a nucleus-encoded protein that is a key activator of mitochondrial transcription as well as a major participant in mitochondrial genome replication. Genomic characterization of the porcine TFAM gene is, therefore, necessary to determine its involvement in regulation of fat depots and meat quality traits in pigs. Approach: Genomic DNA sequence was determined using a comparative in silico annotation approach. RT-PCR was used for analysis of alternative splicing. Genome location was determined using Radiation Hybrid (RH) mapping. Genetic marker was identified by sequencing and genotyped by the PCR-RFLP method with SacI. GENEPOP version 3.3 software was used for statistic analysis. Results: We determined both full-length cDNA and genomic DNA sequences of the porcine TFAM gene. Gene expression analysis revealed an alternative 5' splice site, which excludes exon 4 of the pig gene. We assigned this gene to porcine chromosome 14 (SSC14). A G/A substitution was detected in intron 1 of porcine TFAM gene and genotyped on a total of 252 animals, including 165 from seven Chinese and 87 from five Western pig breeds. The Bayesian analysis via MCMC (Markov chain Monte Carlo) revealed that these two groups of pigs were well separated at this locus during the breed history; 95% of the posterior difference of TFAM allelic frequency between these two pig groups was greater than zero. Conclusion/Recommendations: All these data provided basic genomic information needed for further functional characterization of the porcine TFAM gene. Because marked differences in fat and lean tissue deposition exist between Western and Chinese pig breeds, the G557A mutation in the TFAM gene deserves further evaluation to determine its phenotypic effect on fattening and carcass traits in commercial pig populations.

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Significant associations of the mitochondrial transcription factor A promoter polymorphisms with marbling and subcutaneous fat depth in Wagyu x Limousin F2 crosses

Cited by 50 publications

References 21 publications

Associations between lipid metabolism and fertility in the dairy cow

Associations between lipid metabolism and fertility in the dairy cow

Recent advances in cattle functional genomics and their application to beef quality

The Porcine Mitochondrial Transcription Factor a Gene: Molecular Characterization, Radiation Hybrid Mapping and Genetic Diversity among 12 Pig Breeds

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