Significant Association Between MTHFR C677T Polymorphism and Thyroid Cancer Risk: Evidence from a Meta-Analysis

Chen, Ying; Wang, Bin; Yan, Shushan; Wang, Yangang

doi:10.1089/gtmb.2014.0029

Cited by 3 publications

(2 citation statements)

References 26 publications

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“…FBXW7 mediates the recognition, binding, and targeting substrates for ubiquitin‐mediated degradation . Mutations in these genes have been associated with different types of cancer: PER3 with prostate cancer; MTHFR with leukemia, breast, and thyroid cancer; and FBXW7 with leukemia, cholangiocarcinoma, and breast cancer …”

Section: Methodsmentioning

confidence: 99%

Denaturing high‐performance liquid chromatography and principal component analysis for identification of DNA point mutations in breast cancer and lymphoma samples

Sepúlveda-Villegas

Durán

Villela

et al. 2018

Journal of Chemometrics

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DNA mutations are identified by techniques that use the knowledge of the wild‐type DNA sequence and its mutated variant. The involved analytic methods must be accurate, rapid, and sustainable, if a clinical application is pursued. High‐performance liquid chromatography under denaturing conditions is a useful technique to screen mutations. Denaturing high‐performance liquid chromatography resultant chromatograms are suitable for feature extraction analysis with multivariate methods such as principal component analysis. In this work, principal component analysis was applied to analyze the chromatograms from 3 different genes. Fragments with verified wild‐type sequence were used as reference and samples with sequence unknown were tested. A statistical characterization based on Tukey's boxplot equation of principal component scores allowed us to analyze the distance distribution between reference and sample clusters to establish a classification criterion: an outlier could represent a mutated sample, and a typical value could be a wild‐type sample. Identified outliers were further analyzed by sequencing and proved to carry a mutation. From 72 datasets with a total of 4258 injections, we successfully assessed the classification criterion, identifying mutated samples in lymphoma and breast cancer patients with ratio of prediction Gmean = [0.89, 1.00]. Compared with sequencing analysis, this procedure reduced time and costs.

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Section: Methodsmentioning

confidence: 99%

Denaturing high‐performance liquid chromatography and principal component analysis for identification of DNA point mutations in breast cancer and lymphoma samples

Sepúlveda-Villegas

Durán

Villela

et al. 2018

Journal of Chemometrics

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“…The MTHFR +1298A/C polymorphism (rs1801131) results in a glutamic acid (A)-to-alanine (C) substitution leading to a significant decrease of MTHFR enzyme activity in CC genotype individuals (19). Genetic variation in this gene affects the susceptibility to many types of cancer, including thyroid cancer (20)(21)(22)(23)(24).…”

Section: Introductionmentioning

confidence: 99%

MTHFR gene polymorphisms in hypothyroidism and hyperthyroidism among Jordanian females

Abu-Hassan¹,

Alhouri²,

Altork³

et al. 2019

Archives of Endocrinology and Metabolism

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Objective: Methylenetetrahydrofolate reductase (MTHFR) is involved in DNA methylation that is associated with autoimmune pathology. We investigated the association between MTHFR genetic polymorphisms at g.677C>T and g.1298A>C and their haplotypes, and the risk of thyroid dysfunction among Jordanian females. Subjects and methods: A case-control study involving 98 hypothyroidism cases, 66 hyperthyroidism cases and 100 controls was conducted. Polymerase chain reaction/restriction fragment length polymorphism technique was performed to determine genotypes. Statistical analysis using SPSS software was performed. Results: Genetic analysis showed a significant difference in genotype frequency of g.1298A>C between cases, and controls [hypothyroidism: AA (45.9%), AC (37.8%), CC (16.3%); hyperthyroidism: AA (9.1%), AC (69.7%), CC (21.2%); controls: AA (37.8%), AC (29.6%), CC (32.7%); CC hypo vs. AA hypo : 2.55, 95% CI: (1.18-5.52); OR at least on C hypo : 1.79, 95% CI: (1.07-2.99)]; CC hyper vs. AA hyper : 4.01, 95% CI: (1.79-9.01); OR at least on C hyper : 0.18, 95% CI: (0.07-0.48)]. There was no significant difference in genotype frequency of g.677C>T between cases and controls [hypothyroidism: CC (50.0%), CT (32.7%), TT (17.3%); hyperthyroidism: CC (77.3%), CT (15.2%), TT (7.6%); controls: CC (55.6%), CT (32.3%), TT (12.1%)]. There was a significant difference of MTHFR haplotypes among hypothyroidism cases and controls. TA and CC had a lower hypothyroidism risk whereas; TC showed a higher risk. Conclusions: g.1298A>C genetic polymorphism of MTHFR may modulate the risk of thyroid disease. CC, TA, and TC haplotypes affect the risk of hypothyroidism. Larger samples should be included in the future to verify the role of MTHFR polymorphisms in thyroid diseases.

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Association of Methylenetetrahydrafolate Reductase Gene Polymorphism (MTHFR) in Patients with Gallbladder Cancer

Dixit

Singh

Pandey

et al. 2015

J Gastrointest Canc

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Significant Association Between MTHFR C677T Polymorphism and Thyroid Cancer Risk: Evidence from a Meta-Analysis

Abstract: This meta-analysis suggests that there is a significant association between MTHFR C677T polymorphism and thyroid cancer risk in Caucasians.

Cited by 3 publications

References 26 publications

Denaturing high‐performance liquid chromatography and principal component analysis for identification of DNA point mutations in breast cancer and lymphoma samples

Denaturing high‐performance liquid chromatography and principal component analysis for identification of DNA point mutations in breast cancer and lymphoma samples

MTHFR gene polymorphisms in hypothyroidism and hyperthyroidism among Jordanian females

Association of Methylenetetrahydrafolate Reductase Gene Polymorphism (MTHFR) in Patients with Gallbladder Cancer

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