Significance of Polymorphisms and Expression of Enzyme-Encoding Genes Related to Glutathione in Hematopoietic Cancers and Solid Tumors

Zmorzyński, Szymon; Świderska-Kołacz, Grażyna; Koczkodaj, Dorota; Filip, Agata

doi:10.1155/2015/853573

Cited by 28 publications

(23 citation statements)

References 57 publications

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“…Ki-67 protein is expressed during all active phases of the cell cycle (G 1 , S, G 2 , and mitosis), but is absent from resting cells (G 0 )32. GPX3 gene codes for the Glutathione peroxidase 3, also known as plasma glutathione peroxidase (GPx-P), the variations in activity of GPX1, GPX2, and GPX3 isoforms may be associated with the development of cancers, for example, prostate cancer or even colon cancer39. Leprecan-like 1 is a potential tumor suppressor gene since it has been demonstrated to be downregulated in the hepatocarcinoma tissues and its overexpression inhibits cancer cell proliferation and colony formation through regulation of the cell cycle by downregulation of cyclins40.…”

Section: Discussionmentioning

confidence: 99%

HMGA1P7-pseudogene regulates H19 and Igf2 expression by a competitive endogenous RNA mechanism

Martino

Forzati

Marfella

et al. 2016

Sci Rep

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Recent studies have revealed that pseudogene transcripts can function as competing endogenous RNAs, and thereby can also contribute to cancer when dysregulated. We have recently identified two pseudogenes, HMGA1P6 and HMGA1P7 for the HMGA1 gene whose overexpression has a critical role in cancer progression. These pseudogenes work as competitive endogenous RNA decoys for HMGA1 and other cancer related genes suggesting their role in carcinogenesis. Looking for new HMGA1 pseudogene ceRNAs, we performed RNA sequencing technology on mouse embryonic fibroblasts deriving from transgenic mice overexpressing HMGA1P7. Here, we report that HMGA1P7 mRNA sustains the H19 and Igf2 overexpression by acting as miRNA decoy. Lastly, the expression of HMGA1P7 was significantly correlated with H19 and IGF2 levels in human breast cancer thereby suggesting a role for HMGA1P7 deregulation in this neoplasia.

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Section: Discussionmentioning

confidence: 99%

HMGA1P7-pseudogene regulates H19 and Igf2 expression by a competitive endogenous RNA mechanism

Martino

Forzati

Marfella

et al. 2016

Sci Rep

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“…The GSTO-1 enzyme is responsible for catalyzing the reaction that results from glutathione conjugates generated by GSH in response to damage signals, thus influencing the relationship between reduced glutathione and oxidized glutathione (GSH/GSSG) modulating the expression of cytokines related to the Th1 immune response (Dobashi et al, 2001), thus playing a key role on the host immune response modulation. To date, very few studies have identified the effect of SNP into the gsto-1 sequence (He et al, 2013;Zmorzynski et al, 2015), including teleost species (Liao et al, 2017). The analysis of the four gsto-1 nonsynonymous SNPs found by SIFT and POLYPHEN showed two of them with the second (c.205A > G; c484T > C) and the highest (c.499T > C; c.769A > C) score possible.…”

Section: Discussionmentioning

confidence: 99%

Single-Nucleotide Polymorphisms (SNP) Mining and Their Effect on the Tridimensional Protein Structure Prediction in a Set of Immunity-Related Expressed Sequence Tags (EST) in Atlantic Salmon (Salmo salar)

Vallejos‐Vidal¹,

Reyes-Cerpa²,

Rivas‐Pardo³

et al. 2020

Front. Genet.

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Single-nucleotide polymorphisms (SNPs) are single genetic code variations considered one of the most common forms of nucleotide modifications. Such SNPs can be located in genes associated to immune response and, therefore, they may have direct implications over the phenotype of susceptibility to infections affecting the productive sector. In this study, a set of immune-related genes (cc motif chemokine 19 precursor [ccl19], integrin b2 (itb2, also named cd18), glutathione transferase omega-1 [gsto-1], heat shock 70 KDa protein [hsp70], major histocompatibility complex class I [mhc-I]) were analyzed to identify SNPs by data mining. These genes were chosen based on their previously reported expression on infectious pancreatic necrosis virus (IPNV)-infected Atlantic salmon phenotype. The available EST sequences for these genes were obtained from the Unigene database. Twenty-eight SNPs were found in the genes evaluated and identified most of them as transition base changes. The effect of the SNPs located on the 5'-untranslated region (UTR) or 3'-UTR upon transcription factor binding sites and alternative splicing regulatory motifs was assessed and ranked with a low-medium predicted FASTSNP score risk. Synonymous SNPs were found on itb2 (c.2275G > A), gsto-1 (c.558G > A), and hsp70 (c.1950C > T) with low FASTSNP predicted score risk. The difference in the relative synonymous codon usage (RSCU) value between the variant codons and the wild-type codon (DRSCU) showed one negative (hsp70 c.1950C > T) and two positive DRSCU values (itb2 c.2275G > A; gsto-1 c.558G > A), suggesting that these

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“…There are many polymorphisms in the antioxidant enzymes' genes which may alter their ROS detoxification efficiency. It has been suggested that rs1050450 polymorphism of GPx‐1 gene (leucine for proline substitution in residue 198, in 3, 3p21 region) may affect GPx‐1 enzyme function . Moreover, the rs4880 polymorphism which is located on the second exon of SOD2 gene (6, 6q25 region) and is a functional polymorphism at the 16th amino acid of SOD2 (Val16Ala) could alter the enzyme conformation .…”

Section: Discussionmentioning

confidence: 99%

“…It has been suggested that rs1050450 polymorphism of GPx-1 gene (leucine for proline substitution in residue 198, in 3, 3p21 region) may affect GPx-1 enzyme function. 26 Moreover, the rs4880 polymorphism which is located on the second exon of SOD2 gene (6, 6q25 region) and is a functional polymorphism at the 16th amino acid of SOD2 (Val16Ala) could alter the enzyme conformation. 27 This substitution induces a 30-40% reduction in SOD2 antioxidant activity by the reduction of its transportation into the matrix of mitochondria.…”

Section: Discussionmentioning

confidence: 99%

The effects of the genetic polymorphisms of antioxidant enzymes on susceptibility to papillary thyroid carcinoma

et al. 2020

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Several lines of evidences have indicated that inflammation play an important role in the carcinogenesis. During the inflammatory processes, free radical species are produced from oxidative stress. In normal conditions, enzymatic and nonenzymatic antioxidants remove these products. Manganese superoxide dismutase (MnSOD), glutathione peroxidase‐1 (GPx‐1), and catalase (CAT) are three important enzymes. Therefore, this study aimed to evaluate the effects of MnSOD (SOD2), GPX‐1, and CAT genetic polymorphisms on papillary thyroid carcinoma (PTC) susceptibility. A total of 134 patients with PTC and 151 healthy controls were recruited to participate in this study. All samples were genotyped for SOD2 rs4880, GPX1 1050450, and CAT rs7943316 polymorphisms by polymerase chain reaction‐restriction fragment length polymorphism method. The frequencies of the rs1050450, rs4880, and rs7943316 alleles and genotypes were not different between PTC patients and controls. However, the TC genotype of SOD2 rs4880 polymorphism was significantly higher in males compared to that in females in PTC patients (odds ratio [OR], 3.9 [95% CI, 1.5–11], p = .007). The rs4880 polymorphism was also associated with higher stages (III‐IV) of PTC in dominant model. No significant correlation was found between GPX1‐rs1050450 and CAT‐rs7943316 polymorphisms and demographic, clinical, and pathological features of the disease. The SOD2 rs4880CT genotype was more frequent in males with PTC and patients with higher stages (III–IV) of disease (OR, 2.9 [95% CI, 1.1–7.7], p = .04). However, no significant association was found between GPX1‐rs1050450 and CAT‐rs7943316 variants and PTC or its demographic, clinical, and pathological features.

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Significance of Polymorphisms and Expression of Enzyme-Encoding Genes Related to Glutathione in Hematopoietic Cancers and Solid Tumors

Cited by 28 publications

References 57 publications

HMGA1P7-pseudogene regulates H19 and Igf2 expression by a competitive endogenous RNA mechanism

HMGA1P7-pseudogene regulates H19 and Igf2 expression by a competitive endogenous RNA mechanism

Single-Nucleotide Polymorphisms (SNP) Mining and Their Effect on the Tridimensional Protein Structure Prediction in a Set of Immunity-Related Expressed Sequence Tags (EST) in Atlantic Salmon (Salmo salar)

The effects of the genetic polymorphisms of antioxidant enzymes on susceptibility to papillary thyroid carcinoma

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