Significance of MiB‐1 staining in smears with atypical glandular cells

Abstract

MiB-1 immunostaining may facilitate recognition of developing adenocarcinoma in cervical smear screening. In a retrospective analysis of prospectively collected data of 170 patients with atypical endocervical glandular cells and with repeat smears, archival Papanicolaou-stained smears were restained for MiB-1 and classified for the presence of preneoplastic changes of MiB-1 positive epithelial fragments. The results of classification based on MiB-1 positive epithelial fragments corresponded "roughly" with cytomorphological diagnoses. Of the 38 patients in which the primary smear was found to be MiB-1-positive, 12 of these persisted in the repeat smear. Thirty-eight of the 132 repeat smears of patients originally negative for MiB-1 pathology were positive. Of the total 50 MiB-1 positive repeat smears, four showed adenocarcinoma in situ on cytomorphological grounds. MiB-1 staining enhances detection of (pre)neoplastic changes. This approach does not destroy the morphology of the original smear and can be applied to routine material.

Diagnostic Value of Protein Ki67 (MIB-1) in Atypical Pap Smears of Postmenopausal Women

“…Ki-67 incidence in Pap smear samples with endocervical granular atypia showed that analyzing Ki-67 status for distinguishing neoplastic and non-neoplastic lesions was helpful method (Boon et al, 2004).…”

Immunocytochemical detection of Ki‐67 in Diff‐Quik‐stained cytological smears of canine mammary gland tumours

“…In preliminary experiments, there was no reactivity following 1% acid alcohol treatment prior to immunostaining. Microwave pre‐treatment is essential to immunocytochemistry in previously stained FNA smears both to destain and to enhance immunoreaction 5–7 …”

“…Microwave pre-treatment is essential to immunocytochemistry in previously stained FNA smears both to destain and to enhance immunoreaction. [5][6][7] There has been controversy over the optimal conditions to evaluate Ki-67 in FNA smears in samples from humans. Some authors recommend immediate fixation without air-drying of smears.…”

“…On the other hand cytological samples are easy to obtain in clinical settings. If it is possible to evaluate tumour markers in fine needle aspiration (FNA) smears, they would be a valuable adjunct for evaluation of tumour behaviour 4–17 . Zuccari et al.…”

“…If it is possible to evaluate tumour markers in fine needle aspiration (FNA) smears, they would be a valuable adjunct for evaluation of tumour behaviour. [4][5][6][7][8][9][10][11][12][13][14][15][16][17] Zuccari et al 17 applied Ki-67 antibody to ethanol-fixed imprint smears to show that the marker can be assessed in both FNA smears and histological samples. This study evaluates the possibility of retrieving Ki-67 antigen in Diff-Quik-stained smears from canine mammary gland tumours.…”