Significance of explant preparation and sizing in Aloe vera L.—A highly efficient method for in vitro multiple shoot induction

Singh, B Chandra Sekhar; Sood, Neelu

doi:10.1016/j.scienta.2009.03.023

Cited by 15 publications

(5 citation statements)

References 20 publications

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“…An interesting result was observed in explants preparation, sizing and a single mother plant can be multiplied for 30 to 35 explants per mother plant and 8.84 ± 0.03 shoots per explants were obtained on MS medium supplemented with BAP (1.5 mgL (Balraj and Neelu, 2009). It was also reported that the enhancement of shoots was observed by using BA and NAA.…”

Section: Shoot Multiplicationmentioning

confidence: 89%

A method of rapid in vitro proliferation and morphological characterization of the medicinal plant Aloe vera L.

Singh¹,

Adugna²,

Rani³

2017

Afr. J. Biotechnol.

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Section: Shoot Multiplicationmentioning

confidence: 89%

A method of rapid in vitro proliferation and morphological characterization of the medicinal plant Aloe vera L.

Singh¹,

Adugna²,

Rani³

2017

Afr. J. Biotechnol.

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“…At the early stages of plant tissue culture, scientists observed that the size of the explant is closely linked to the final outcome of tissue culture [36]. The selection of explant size is a crucial factor that affects the success of tissue culture procedures [37,38]. The size of the explant can impact nutrient absorption, growth rate, and differentiation [39].…”

Section: Discussionmentioning

confidence: 99%

The Influence of Maturity, Storage, and Embryo Size on Coconut Callus Induction Success

Mu,

Yang,

et al. 2024

Forests

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Coconut palms (Cocos nucifera L.) are globally significant palms with both economic and cultural value. Despite the increasing demand for coconut products, production is decreasing globally due to palm senility, pests, and diseases. It has been estimated that over half of the world’s coconut palms need to be replaced immediately. The coconut industry has acknowledged that conventional propagation methods are unlikely to yield sufficient high-quality planting material. Therefore, coconut tissue culture is considered a potential solution to this problem. By using coconut tissue culture, a large number of plantlets can be obtained in a short period of time. In this study, the quality of explants and the development stage (visible shoot/non-visible shoot) of coconut used for micropropagation were examined. To our knowledge, little research has been undertaken on this aspect of coconut micropropagation. Our results indicated that tender coconut fruit exhibited an advantage over mature fruits. In addition, coconut plumule explants subjected to an extended storage of 15 days demonstrated enhanced development compared to those without storage. Notably, smaller embryos utilized as explants displayed superior callus formation compared to their larger counterparts. Finally, embryos possessing shoots exhibited improved callus initiation, albeit accompanied by a more pronounced browning effect. Further investigations are required to obtain more knowledge about the most suitable conditions for plumule explants that lead to optimal callus initiation.

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“…Before culture, this study used double sterilization with NaOCl to effectively sterilize explants, including seeds obtained from the field under ambient conditions, which may be highly susceptible to contaminations. Even though NaOCl was used, double sterilization of field-grown Aloe vera was effectively decontaminated before culture using 0.1% mercuric chloride and 0.04% streptomycin in 5-6 min (Singh and Sood 2009). Coatless seeds and embryos were not immersed in the sterilant after isolation since they are well protected in intact double sterilized seeds.…”

Section: Decontamination Of Explantsmentioning

confidence: 99%

Effective decontamination and multiplication of Croton membranaceus in vitro

2022

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Abstract. Adukonu IA, Ayeh KO, Acheampong E. 2022. Effective decontamination and multiplication of Croton membranaceus in vitro. Cell Biol Dev 6: 68-81. Croton membranaceus Müll. Arg. is a useful herb with medicinal properties, and its leaves, roots, and bark are used to treat diverse ailments. However, the harvesting method by traditional medical practitioners without replacement exposes it to extinction. Therefore, the only means of propagating is by relatively slow use of seeds, and an alternative propagation method is needed for field establishment and nursery. Therefore, this study aims at determining an effective propagation by sterilization regime and subsequent in vitro regeneration using different explants; intact seeds, isolated embryos, coatless seeds, and nodal-cutting. The explants were decontaminated using double sterilization; the best was achieved by pre-treatment with 70% ethanol for 3 min before immersion in sodium hypochlorite (NaOCl). Intact seeds were effectively decontaminated by immersion in 15% NaOCl solution for 20 min, then by 10% NaOCl for 15 min. Conversely, coatless seeds were effectively decontaminated when isolated from intact seeds immersed in 20% NaOCl solution for 20 min and 15% NaOCl for 15 min. Further, embryos isolated from intact seeds were effectively decontaminated in 20% NaOCl for 15 min, then by 15% NaOCl for 10 min sequentially. These sterilization regimes successfully decontaminated 86% intact seeds, 100% isolated embryos, and 80% coatless seeds. Nodal-cutting explants were best decontaminated by immersion in 20% NaOCl solution for 15 min, followed by 15% NaOCl for 10 min sequentially without ethanol pre-treatment. This sterilization regime successfully decontaminated 100% of the nodal-cutting explants. However, the development of shoot explants varied in response to sterilization. Intact seeds did not develop into shoots, while isolated embryos, coatless seeds, and nodal-cutting explants developed into shoots independent of the sterilization regime. Shoot development was highest with the medium’s shoot-tip explants added with BAP, NAA, and GA3. Shoot multiplication was best achieved on an MS basal medium with 5.0 ?M BAP, 0.5 ?M NAA, and 5.0 ?M GA3 amendment.

show abstract

Significance of explant preparation and sizing in Aloe vera L.—A highly efficient method for in vitro multiple shoot induction

Cited by 15 publications

References 20 publications

A method of rapid in vitro proliferation and morphological characterization of the medicinal plant Aloe vera L.

A method of rapid in vitro proliferation and morphological characterization of the medicinal plant Aloe vera L.

The Influence of Maturity, Storage, and Embryo Size on Coconut Callus Induction Success

Effective decontamination and multiplication of Croton membranaceus in vitro

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