A method of rapid in vitro proliferation and morphological characterization of the medicinal plant Aloe vera L.

Singh, B Chandra Sekhar; Adugna, A Diriba; Rani, Anita

doi:10.5897/ajb2017.16242

Cited by 5 publications

(2 citation statements)

References 27 publications

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“…There are several reports about genetic diversity, germplasm or seed of plant (Admas and Tesfaye, 2017;Ali et al, 2018;Arantes et al, 2018;Chalachew et al, 2017;Chandra et al, 2017;Daniel et al, 2017;Dilooshi et al, 2016;Santos et al, 2017;Ejigu et al, 2018;Emmanuel et al, 2018;Isaac et al, 2016;Lopez-Puc and Rodriguez-Buenfil, 2017;Revolti et al, 2018;Miccah et al, 2016;Mohammad et al, 2016;Mounawer et al, 2016;Mulima et al, 2018;Ochieng et al, 2015;Pacôme et al, 2016;Raimundo et al, 2015;Rodrigues et al, 2016;Tadesse et al, 2018;Titilayo et al, 2018;Zerihun et al, 2018), fungi (Edelvio, 2018;Ling et al, 2016;Shubha and Srinivas, 2017), bacteria (Chaohe et al, 2015;Paul et al, 2016), and virus (Chikoti et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Seed germination of seeds preserved for about 20 years and that of seeds preserved for several years

Park¹,

Jeong²,

Kim³

et al. 2019

Afr. J. Agric. Res.

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From the herbage seeds, only alfalfa (Medicago sativa) showed about 3-5% germination ratio for 20 years old seeds. The results are consistent with that of Park and Kim (2009). No germination was observed from the other herbage species, but the decay of seeds were observed sometimes. However, the vegetable seeds preserved for several years showed high germination ratios. In conclusion, older seeds generally germinate a little, while alfalfa seeds continue to live for long time. In addition, new seeds generally germinate more, while, for example, young summer radish germinated a little.

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Section: Introductionmentioning

confidence: 99%

Seed germination of seeds preserved for about 20 years and that of seeds preserved for several years

Park¹,

Jeong²,

Kim³

et al. 2019

Afr. J. Agric. Res.

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show abstract

“…Plant tissue culture technique would be a sustainable solution in expanding A. vera cultivation through the production of uniform seedlings of desirable genotype. Several attempts have been taken to develop micropropagation of Aloe spp [3][4][5][6][7][8][9][10] . However, quality of the seedlings for possible genetic changes was rarely tested.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of genetic fidelity of in vitro-propagated Aloe vera plants using DNA-based markers

Khatun¹,

Tanny²,

Yesmin³

et al. 2018

ScienceAsia

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Aloe vera is a world-recognized medicinal herb cultivated for its high value in medicine and the cosmetics industry. Natural multiplication of this plant through suckers is inadequate to meet the demand of high quality planting material for commercial cultivation. Earlier we developed an efficient micropropagation protocol using shoot tip explants of field grown seedlings. Field trials of the micropropagated plants reveal no morphological or growth abnormalities. For commercial utility it is therefore imperative to establish genetic uniformity of micropropagated plants to confirm the true-to-type plantlets. Hence the present investigation assesses the clonal fidelity of the in vitro A. vera plants using polymerase chain reaction-based techniques such as random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR). In this study, we assessed the suitability of 20 RAPD and 12 ISSR primers to observe any polymorphism. Out of these, 12 RAPD and 8 ISSR primers produced resolvable, reproducible and scorable bands. The 12 RAPD primers produced 75 distinct and scorable bands with an average of 6.25 bands, and 8 ISSR primers produced 31 distinct and scorable bands with an average of 3.8 bands per primer. RAPD and ISSR profiles obtained through the amplification of genomic DNA of the in vitro grown A. vera plants were similar in all aspects. No RAPD and ISSR polymorphism in the micropropagated plants were detected. This implies that micropropagation through shoot tip explants is a safe method for producing true-to-type plants and could be used for commercial plantation of A. vera.

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Amelioration of Morpho-structural and Physiological Disorders in Micropropagation of Aloe vera L. by Use of an Aromatic Cytokinin 6-(3-Hydroxybenzylamino) Purine

Manokari

Priyadharshini

Cokulraj

et al. 2022

J Plant Growth Regul

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A method of rapid in vitro proliferation and morphological characterization of the medicinal plant Aloe vera L.

Cited by 5 publications

References 27 publications

Seed germination of seeds preserved for about 20 years and that of seeds preserved for several years

Seed germination of seeds preserved for about 20 years and that of seeds preserved for several years

Evaluation of genetic fidelity of in vitro-propagated Aloe vera plants using DNA-based markers

Amelioration of Morpho-structural and Physiological Disorders in Micropropagation of Aloe vera L. by Use of an Aromatic Cytokinin 6-(3-Hydroxybenzylamino) Purine

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