Significance of Brownian Motion for Nanoparticle and Virus Capture in Nanocellulose-Based Filter Paper

Abstract

Pressure-dependent breakthrough of nanobioparticles in filtration was observed and it was related to depend on both convective forces due to flow and diffusion as a result of Brownian motion. The aim of this work was to investigate the significance of Brownian motion on nanoparticle and virus capture in a nanocellulose-based virus removal filter paper through theoretical modeling and filtration experiments. Local flow velocities in the pores of the filter paper were modeled through two different approaches (i.e., with the Hagen–Poiseuille equation) and by evaluating the superficial linear flow velocity through the filter. Simulations by solving the Langevin equation for 5 nm gold particles and 28 nm ΦX174 bacteriophages showed that hydrodynamic constraint is favored for larger particles. Filtration of gold nanoparticles showed no difference in retention for the investigated fluxes, as predicted by the modeling of local flow velocities. Filtration of ΦX174 bacteriophages exhibited a higher retention at higher filtration pressure, which was predicted to some extent by the Hagen–Poiseuille equation but not by evaluation of the superficial linear velocity. In all, the hydrodynamic theory was shown able to explain some of the observations during filtration.

Aggregate Removal Nanofiltration of Human Serum Albumin Solution Using Nanocellulose-Based Filter Paper

“…Better virus clearance at an overhead pressure ≥3 bar is concordant with previously published data [34]. The observed improved clearance at higher pressure is related to a combined effect of compaction of the filter at higher pressure [45] and decreased tendency to Brownian motion [46].…”

Versatile Application of Nanocellulose: From Industry to Skin Tissue Engineering and Wound Healing

“…Algae as sources of nanocellulose are Cladophora [ 37 , 38 , 83 , 84 , 85 , 86 , 87 ] and Cystoseria myrica [ 88 ]. Nanocellulose materials derived from Cladophora have been tested mainly for their potential biomedical applications in terms of the presence of impurities, such as heavy metals, glucans, and endotoxins [ 85 ].…”

Two-Step Size-Exclusion Nanofiltration of Prothrombin Complex Concentrate Using Nanocellulose-Based Filter Paper

“…The pore size and flux properties of the filter paper can be controlled, which opens new opportunities to model fundamental aspects of bioprocessing [32][33][34]. The filter paper was previously validated in numerous studies to remove several large and small-size model viruses, including retroviruses (xMuLV, 100 nm) [35], parvoviruses (MVM, 20 nm) [32,36], and model phages (ΦX174, 28 nm) [37][38][39]. Recently, it was shown that this nanocellulose-based virus removal filter paper is useful for bioprocessing human plasma-derived IgG [40].…”