Significado pronóstico del síndrome hepatopulmonar en la cirrosis hepática

Martínez, J. L. Alonso; Urmeneta, José Manuel Zozaya; Dubois, J. Gutiérrez; Echezarreta, M. A. Urbieta; Sánchez, F.J. Anniccherico

doi:10.1157/13090378

Cited by 8 publications

(2 citation statements)

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“…However, the cellular signaling mechanisms involved in HPS–associated pulmonary vascular remodeling are still poorly understood. Previous studies and our initial research have demonstrated that the differentiation and proliferation of PMVECs play an important role in the pathogenesis of IPVD of HPS and that cytoskeletal remodeling is the key change in the pathophysiology of the proliferation or differentiation of PMVECs [1,3,18,19]. Whether the regulation of cytoskeletal proteins in PMVECs is a good therapeutic approach demands further investigation.…”

Section: Discussionmentioning

confidence: 99%

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Annexin A1 protein regulates the expression of PMVEC cytoskeletal proteins in CBDL rat serum-induced pulmonary microvascular remodeling

Zeng

Wang

et al. 2013

J Transl Med

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BackgroundHepatopulmonary syndrome (HPS) is characterized by advanced liver disease, hypoxemia and intrapulmonary vascular dilatation (IPVD). The pathogenesis of HPS is not completely understood. Recent findings have established the role of proliferation and phenotype differentiation of pulmonary microvascular endothelial cells (PMVECs) in IPVD of HPS; the change in cytoskeletal proteins and their molecular mechanism play an essential role in the proliferation, phenotype modulation and differentiation of PMVECs. However, little is known about the relevance of cytoskeletal protein expression and its molecular mechanism in IPVD of HPS. In addition, ANX A1 protein has been identified as a key regulator in some important signaling pathways, which influences cytoskeletal remodeling in many diseases, such as lung cancer, liver cancer, etc.MethodsPMVECs were cultured from the normal rats and then divided into three groups(Ad-ANXA1-transfected group, a non-transfected group, and an adenovirus empty vector group) and incubated by nomal rat serum or hepatopulmonary syndrome rat serum respectively. mRNA level was evaluated by real time reverse transcription polymerase chain reaction, and protein expression was detected by western blot. Cell proliferation was determined by the MTT and thymidine incorporation assay.ResultsIn this study, we found that the serum from a common bile duct ligation(CBDL) Rat model decreased the expression levels of the ANX A1 mRNA and protein by at least two-fold in human PMVECs. We also found the expression of cytoskeletal proteins (Destrin, a1-actin, and a1-tubulin) in PMVECs significantly increased. After stimulating ANX A1 over-expression in PMVECs by adenovirus-mediated ANX A1 (Ad-ANXA1) transfection, we found the expression levels of cytoskeletal proteins were significantly suppressed in PMVECs at all time points. Additionally, we report here that serum from a CBDL Rat model increases the proliferation of PMVECs by nearly two-fold and that over-expression of Ad-ANXA1 significantly inhibits HPS-rat-serum-induced PMVEC proliferation (p <0.05). These findings suggest that the ANX A1 down-regulation of PMVEC proliferation in the presence of HPS-rat-serum may be the major cause of aberrant dysregulation of cytoskeletal proteins (Destrin, a1-actin, and a1-tubulin) and may, therefore, play a fundamental role in the proliferation and phenotype differentiation of PMVECs in the PVD of HPS.ConclusionFinally, the fact that transfection with Ad-ANXA1 results in inhibition of the aberrant dysregulation of cytoskeletal proteins and proliferation of PMVECs suggests a potential therapeutic effect on PVD of HPS.

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Section: Discussionmentioning

confidence: 99%

“…Mortality within a year of diagnosis is markedly increased in HPS patients with cirrhotic compared to patients without cirrhotic. [2,3]. There is currently no effective medical treatment for HPS.…”

Section: Introductionmentioning

confidence: 99%