Signals, Regulatory Networks, and Materials That Build and Break Bacterial Biofilms

Karatan, Ece; Watnick, Paula I.

doi:10.1128/mmbr.00041-08

Cited by 845 publications

(713 citation statements)

References 366 publications

(494 reference statements)

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“…In fact, even suspended aggregates of cells display many of the characteristics that are associated with biofilms [61]. The E. coli bacteria in our experiments aggregated and seemed to form 'microcolonies' upon adding NC at a very narrow range of concentration, equivalent to 36 -72 NC/bacterium ( Figure S4).…”

Section: Effect Of Nanocapsules On the Bacterial Quorum Sensing Activmentioning

confidence: 76%

An investigation of the interactions between an E. coli bacterial quorum sensing biosensor and chitosan-based nanocapsules

Qin

Engwer

Desai

et al. 2017

Colloids and Surfaces B: Biointerfaces

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Highlights Chitosan-coated nanocapsules, but not nanoemulsions, influence the -potential of E.coli  A fixed number of nanocapsules binds and promotes aggregation of bacteria  In silico simulations agree closely with experimental results  Chitosan-coated nanocapsules attenuate the bacterial quorum sensing response § These authors contributed equally to this publication * Author for correspondence Colloids and Surfaces B: Biointerfaces 149 (2017) 358-368 AbstractWe examined the interaction between chitosan-based nanocapsules (NC), with average hydrodynamic diameter ~114-155 nm, polydispersity ~0.127, and -potential ~+50 mV, and an E. coli bacterial quorum sensing reporter strain. Dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA) allowed full characterization and assessment of the absolute concentration of NC per unit volume in suspension. By centrifugation, DLS, and NTA, we determined experimentally a "stoichiometric" ratio of ~80 NC/bacterium. By SEM it was possible to image the aggregation between NC and bacteria. Moreover, we developed a custom in silico platform to simulate the behavior of particles with diameters of 150 nm and -potential of +50 mV on the bacterial surface. We computed the detailed force interactions between NC-NC and NC-bacteria and found that a maximum number of 145 particles might interact at the bacterial surface. Additionally, we found that the "stoichiometric" ratio of NC and bacteria has a strong influence on the bacterial behavior and influences the quorum sensing response, particularly due to the aggregation driven by NC.

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Section: Effect Of Nanocapsules On the Bacterial Quorum Sensing Activmentioning

confidence: 76%

An investigation of the interactions between an E. coli bacterial quorum sensing biosensor and chitosan-based nanocapsules

Qin

Engwer

Desai

et al. 2017

Colloids and Surfaces B: Biointerfaces

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“…alone (Stavridou and Forzi, 2011). The formation of biofilms is thought to be governed by a diverse number of factors, including nutritional availability (Lim et al 2004), osmolarity (Karatan and Watnick, 2009), self-generated quorum sensing signals (De Kievit, 2009) and the chemistry and topography of the host surface (MacKintosh et al 2006). Much work has gone into trying to disrupt or prevent biofilm formation, some of which has focussed on using ultrasound (Hazan et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Acoustic vibration can enhance bacterial biofilm formation

Murphy

Edwards

Hobbs

et al. 2016

Journal of Bioscience and Bioengineering

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“…membrane or periplasmic protein composition, lipid composition, periplasmic glucan level and EPS synthesis (Miller & Wood, 1996). Ultimately, some of these modifications could lead to biofilm (or aggregate) formation (Karatan & Watnick, 2009). …”

Section: Discussionmentioning

confidence: 99%

The two-component system PrlS/PrlR of Brucella melitensis is required for persistence in mice and appears to respond to ionic strength

et al. 2012

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Bacterial adaptation to environmental conditions is essential to ensure maximal fitness in the face of several stresses. In this context, two-component systems (TCSs) represent a predominant signal transduction mechanism, allowing an appropriate response to be mounted when a stimulus is sensed. As facultative intracellular pathogens, Brucella spp. face various environmental conditions, and an adequate response is required for a successful infection process. Recently, bioinformatic analysis of Brucella genomes predicted a set of 15 bona fide TCS pairs, among which some have been previously investigated. In this report, we characterized a new TCS locus called prlS/R, for probable proline sensor-regulator. It encodes a hybrid histidine kinase (PrlS) with an unusual Na + /solute symporter N-terminal domain and a transcriptional regulator (belonging to the LuxR family) (PrlR). In vitro, Brucella spp. with a functional PrlR/S system form bacterial aggregates, which seems to be an adaptive response to a hypersaline environment, while a prlS/R mutant does not. We identified ionic strength as a possible signal sensed by this TCS. Finally, this work correlates the absence of a functional PrlR/S system with the lack of hypersaline-induced aggregation in particular marine Brucella spp.

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Signals, Regulatory Networks, and Materials That Build and Break Bacterial Biofilms

Cited by 845 publications

References 366 publications

An investigation of the interactions between an E. coli bacterial quorum sensing biosensor and chitosan-based nanocapsules

An investigation of the interactions between an E. coli bacterial quorum sensing biosensor and chitosan-based nanocapsules

Acoustic vibration can enhance bacterial biofilm formation

The two-component system PrlS/PrlR of Brucella melitensis is required for persistence in mice and appears to respond to ionic strength

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