Acoustic vibration can enhance bacterial biofilm formation

Murphy, M. F.; Edwards, Thomas; Hobbs, Glyn; Shepherd, Joanna; Bezombes, Frédéric

doi:10.1016/j.jbiosc.2016.05.010

Cited by 27 publications

(32 citation statements)

References 21 publications

(14 reference statements)

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“…Limitations of this study consist of the clinical translatability of the results, including biofilm maturation and heterogeneity, biofilm quantification methods, the size, and shape of the coupons as compared to prosthetics, the duration of treatment exposure, and the cytotoxicity of these topical adjuvants to healthy cells. Although it has been shown that mature biofilm can be established in vitro following 48 h incubation, clinical presentations of biofilm‐related joint infection generally consist of long‐established biofilm . However, the clinical application of adjuvant treatments as described here would be considered in the setting of acute infections.…”

Section: Discussionmentioning

confidence: 99%

Topical adjuvants incompletely remove adherent Staphylococcus aureus from implant materials

Ernest

Machi

Karolcik

et al. 2017

Journal Orthopaedic Research

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Adjuvant treatments including Betadine, Dakin's solution (sodium hypochlorite), or hydrogen peroxide (H O ) have been attempted to eradicate prosthetic joint infection caused by biofilm or intracellular bacteria. The purpose of this study was to evaluate the in vitro abilities of chemical adjuvants to decrease Staphylococcus aureus (S. aureus) biofilm presence on orthopaedic implant grade materials, including titanium, stainless steel, and cobalt chrome. S. aureus biofilms were grown for 48 h and evaluated for baseline colony forming units/centimeter squared (CFU/cm ) and compared to treatments with Betadine, Dakin's solution, H O , or 1% chlorine dioxide (ClO ). Control discs (n = 18) across all metals had an average of 4.2 × 10 CFU/cm . All treatments had statistically significant reductions in CFU/cm when compared to respective control discs (p < 0.05). For all metals combined, the most efficacious treatments were Betadine and H O , with an average 98% and 97% CFU/cm reduction, respectively. There were no significant differences between reductions seen with Betadine and H O , but both groups had statistically greater reductions than Dakin's solution and ClO . There was no change in antibiotic resistance patterns after treatment. Analysis of S. aureus biofilms demonstrated a statistically significant reduction in biofilm after a five-minute treatment with the modalities, with an average two log reduction in CFU/cm . Statement of clinical significance: While statistically significant reductions in CFU/cm were accomplished with chemical adjuvant treatments, the overall concentration of bacteria never fell below 10 CFU/cm , leading to questionable clinical significance. Further techniques to eradicate biofilm should be investigated. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:1599-1604, 2018.

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Section: Discussionmentioning

confidence: 99%

Topical adjuvants incompletely remove adherent Staphylococcus aureus from implant materials

Ernest

Machi

Karolcik

et al. 2017

Journal Orthopaedic Research

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“…For example, it was shown recently that fluid flows control the microscopic structure and 3D morphology of biofilms ( 29 ). Other mechanical factors, such as mechanical vibration of the substrate, also affect bacterial attachment, leaving a footprint on the biofilms ( 30 ).…”

Section: Introductionmentioning

confidence: 99%

Surface waves control bacterial attachment and formation of biofilms in thin layers

et al. 2020

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Formation of bacterial biofilms on solid surfaces within a fluid starts when bacteria attach to the substrate. Understanding environmental factors affecting the attachment and the early stages of the biofilm development will help develop methods of controlling the biofilm growth. Here, we show that biofilm formation is strongly affected by the flows in thin layers of bacterial suspensions controlled by surface waves. Deterministic wave patterns promote the growth of patterned biofilms, while wave-driven turbulent motion discourages patterned attachment of bacteria. Strong biofilms form under the wave antinodes, while inactive bacteria and passive particles settle under nodal points. By controlling the wavelength, its amplitude, and horizontal mobility of the wave patterns, one can shape the biofilm and either enhance the growth or discourage the formation of the biofilm. The results suggest that the deterministic wave-driven transport channels, rather than hydrodynamic forces acting on microorganisms, determine the preferred location for the bacterial attachment.

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“…For soil bacteria, protozoa are a major source of mortality, and thus defenses against these predators are present in the form of chemical defenses, surface modification, micro-colony formation, and increased motility [11]. Bacterial biofilm formation has also been shown to be sensitive to vibrations under controlled conditions [12]. Clearly, flight responses could be triggered by sound, and to the extent that these substances are inducible and not constitutively expressed, also secondary metabolites functioning in defense might be upregulated.…”

Section: How Sound Might Carry Useful Information For Soil Biotamentioning

confidence: 99%

Sounds of Soil: A New World of Interactions under Our Feet?

Rillig

Bonneval²,

Lehmann

2019

Soil Systems

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Soils are biodiversity-dense and constantly carry chemical flows of information, with our mental image of soil being dark and quiet. But what if soil biota tap sound, or more generally, vibrations as a source of information? Vibrations are produced by soil biota, and there is accumulating evidence that such vibrations, including sound, may also be perceived. We here argue for potential advantages of sound/vibration detection, which likely revolve around detection of potential danger, e.g., predators. Substantial methodological retooling will be necessary to capture this form of information, since sound-related equipment is not standard in soils labs, and in fact this topic is very much at the fringes of the classical soil research at present. Sound, if firmly established as a mode of information exchange in soil, could be useful in an 'acoustics-based' precision agriculture as a means of assessing aspects of soil biodiversity, and the topic of sound pollution could move into focus for soil biota and processes.

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Acoustic vibration can enhance bacterial biofilm formation

Cited by 27 publications

References 21 publications

Topical adjuvants incompletely remove adherent Staphylococcus aureus from implant materials

Topical adjuvants incompletely remove adherent Staphylococcus aureus from implant materials

Surface waves control bacterial attachment and formation of biofilms in thin layers

Sounds of Soil: A New World of Interactions under Our Feet?

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