Signaling by Antibodies: Recent Progress

Bournazos, Stylianos; Wang, Taia T.; Dahan, Rony; Maamary, Jad; Ravetch, Jeffrey V.

doi:10.1146/annurev-immunol-051116-052433

Cited by 178 publications

(196 citation statements)

References 151 publications

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Section: Igg Fc Domain Effector Functionsmentioning

confidence: 99%

“…FcγRs comprise a family of immunoreceptors and are broadly divided into two main types: Type I and II, with each type having unique structural and functional characteristics [17](Figure 1). Upon crosslinking by the Fc domains of IgG immune complexes, FcγRs trigger signaling events through their intracellular signaling motifs, inducing diverse immunomodulatory processes that readily influence the functional activity of effector leukocytes and consequently several aspects of the innate and adaptive immune response [17]. For example, ITAM (immunoreceptor tyrosine-based activation motif)-containing, Type I FcγRs induce the activation of signaling pathways with pro-inflammatory biological consequences, including cellular activation, antibody-dependent cellular cytotoxicity (ADCC), phagocytosis, as well as expression and release of inflammatory cytokines and chemokines.…”

Section: Igg Fc Domain Effector Functionsmentioning

confidence: 99%

“…For example, ITAM (immunoreceptor tyrosine-based activation motif)-containing, Type I FcγRs induce the activation of signaling pathways with pro-inflammatory biological consequences, including cellular activation, antibody-dependent cellular cytotoxicity (ADCC), phagocytosis, as well as expression and release of inflammatory cytokines and chemokines. These activities are counterbalanced by the inhibitory Type I FcγR, FcγRIIb, which limits ITAM-mediated signaling in effector leukocytes [17]. Likewise, engagement of Type II FcγRs by the IgG Fc domain has pleiotropic immunomodulatory effects.…”

Section: Igg Fc Domain Effector Functionsmentioning

confidence: 99%

“…Given the capacity of Type I and Type II FcγRs to activate diverse immunomodulatory pathways upon engagement, Fc-FcγR interactions are dynamically regulated through specific modulation of the Fc domain structure, either in the primary amino acid sequence (IgG subclasses) or in the Fc-associated glycan composition [17,20,21]. Such differences in the IgG subclass and Fc domain glycan structure contribute to substantial Fc domain heterogeneity and it is estimated that over 10 3 Fc domain variants exist, each with differential FcγR affinity and immunomodulatory potential.…”

Section: Igg Fc Domain Effector Functionsmentioning

confidence: 99%

“…Such differences in the IgG subclass and Fc domain glycan structure contribute to substantial Fc domain heterogeneity and it is estimated that over 10 3 Fc domain variants exist, each with differential FcγR affinity and immunomodulatory potential. For example, IgG glycan variants lacking the branching fucose residue (afucosylated) exhibit improved cytotoxic activity compared to their fucosylated counterparts through enhanced capacity to interact with and activate FcγRIIIa-expressing effector leukocytes [17,22,23]. Likewise, the presence of terminal sialic acid residues at the Fc-associated glycan structure determines the binding specificity of the Fc domain for Type I and Type II FcγRs [17].…”

Section: Igg Fc Domain Effector Functionsmentioning

confidence: 99%

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Immunological responses to influenza vaccination: lessons for improving vaccine efficacy

Wang

Bournazos

Ravetch

2018

Current Opinion in Immunology

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A critical factor in the maturation of influenza vaccine responses is the nearly inevitable binding of vaccine antigens by exiting anti-influenza IgGs. These antigen-IgG immune complexes direct the response to immunization by modulating cellular processes that determine antibody and T-cell repertoires: maturation of dendritic cells, processing and presentation of antigens to T cells, trafficking of antigens to the germinal center, and selection of B cells for antibody production. By focusing on the recent advances in the study of the immunomodulatory processes mediated by IgG immune complexes upon influenza vaccination, we discuss a pathway that is critical for modulating the breadth and potency of anti-HA antibody responses and has previously led to the development of strategies to improve influenza vaccine efficacy.

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Section: Igg Fc Domain Effector Functionsmentioning

confidence: 99%

Section: Igg Fc Domain Effector Functionsmentioning

confidence: 99%

Section: Igg Fc Domain Effector Functionsmentioning

confidence: 99%

Section: Igg Fc Domain Effector Functionsmentioning

confidence: 99%

Section: Igg Fc Domain Effector Functionsmentioning

confidence: 99%

See 3 more Smart Citations

Immunological responses to influenza vaccination: lessons for improving vaccine efficacy

Wang

Bournazos

Ravetch

2018

Current Opinion in Immunology

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Optimization of peripheral blood volume for in silico reconstitution of the human B‐cell receptor repertoire

et al. 2022

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B cells recognize antigens via membrane‐expressed B‐cell receptors (BCR) and antibodies. Similar human BCR sequences are frequently found at a significantly higher frequency than that theoretically calculated. Patients infected with SARS‐CoV2 and HIV or with autoimmune diseases share very similar BCRs. Therefore, in silico reconstitution of BCR repertoires and identification of stereotypical BCR sequences related to human pathology have diagnostic potential. Furthermore, monitoring changes of clinically significant BCR sequences and isotype conversion has prognostic potential. For BCR repertoire analysis, peripheral blood (PB) is the most convenient source. However, the optimal human PB volume for in silico reconstitution of the BCR repertoire has not been studied in detail. Here, we sampled 5, 10, and 20 mL PB from the left arm and 40 mL PB from the right arm of two volunteers, reconstituted in silico PB BCR repertoires, and compared their composition. In both volunteers, PB sampling over 20 mL resulted in slight increases in functional unique sequences (FUSs) or almost no increase in repertoire diversity. All FUSs with a frequency above 0.08% or 0.03% in the 40 mL PB BCR repertoire were detected even in the 5 mL PB BCR repertoire from each volunteer. FUSs with a higher frequency were more likely to be found in BCR repertoires from reduced PB volume, and those coexisting in two repertoires showed a statistically significant correlation in frequency irrespective of sampled anatomical site. The correlation was more significant in higher‐frequency FUSs. These observations support the potential of BCR repertoire analysis for diagnosis.

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Electrochemical Synthesis of 3D Plasmonic‐Molecule Nanocomposite Materials for In Situ Label‐Free Molecular Detections

Ansah

Yang

et al. 2021

Adv Materials Inter

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reliability for surface-enhanced Raman scattering (SERS)-based molecular detection. Therefore, the precise placement of infinitesimal molecules in a 3D bulk solution at the hotspots remains a goal for attaining both ultrasensitive and reproducible labelfree molecular detection. Some approaches for positioning target molecules have been proposed, including enhancing molecular interactions using bioreceptors [4][5][6] and carrying out electrokinetic actuations. [7][8][9] The receptor molecules provide specific binding sites for target molecules. However, because the binding events between receptor and target molecules are highly dependent on the molecular diffusion of target molecules in the bulk solution, real-time detection is difficult to achieve using this passive diffusion process. For solution-based detection systems, electrokinetic actuation is considered a promising method to concentrate charged small molecules at hotspot regions through electrophoresis. [7][8][9] However, because of the size mismatch between nanoscale hotspots and large macrobiomolecules, these conventional SERS platforms are not well adapted to label-free and rapid detections of respiratory viruses. Although ≈100 nm virion particles can be attracted to a SERS-active surface via electrophoresis, they may not fit onto nanoscale hotspots because of their structural complexity and large size.

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Signaling by Antibodies: Recent Progress

Cited by 178 publications

References 151 publications

Immunological responses to influenza vaccination: lessons for improving vaccine efficacy

Immunological responses to influenza vaccination: lessons for improving vaccine efficacy

Optimization of peripheral blood volume for in silico reconstitution of the human B‐cell receptor repertoire

Electrochemical Synthesis of 3D Plasmonic‐Molecule Nanocomposite Materials for In Situ Label‐Free Molecular Detections

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