Signal transduction and metabolic flux of β-thujaplicin and monoterpene biosynthesis in elicited Cupressus lusitanica cell cultures

Zhao, Jian; Matsunaga, Yuki; Fujita, Kazuo; Sakai, Kokki

doi:10.1016/j.ymben.2005.09.002

Cited by 24 publications

(23 citation statements)

References 46 publications

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“…In our cell line, it was demonstrated that GPP was converted to b-thujaplicin via a menthane-type skeleton intermediate (Fujita et al, 2000) and stimulation of b-thujaplicin and monoterpene productions are mediated by similar signaling pathways (Zhao et al, 2006). Nevertheless, little is known about the intermediates and enzymes responsible for b-thujaplicin biosynthesis.…”

Section: Introductionmentioning

confidence: 91%

Volatile and non-volatile monoterpenes produced by elicitor-stimulated Cupressus lusitanica cultured cells

Alwis

Fujita

Ashitani

et al. 2009

Journal of Plant Physiology

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Section: Introductionmentioning

confidence: 91%

Volatile and non-volatile monoterpenes produced by elicitor-stimulated Cupressus lusitanica cultured cells

Alwis

Fujita

Ashitani

et al. 2009

Journal of Plant Physiology

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“…Elicitor signal transduction pathways, GTP-binding protein, Ca 2+ flux, IP 3 , and cAMP, H 2 O 2 , as well as jasmonate and ethylene signaling that mediate yeast elicitor-induced -thujaplicin production have been identified [135,279,280,281,282,283,284]. Studies also revealed the metabolic flux and involved signal transduction mechanism for differential biosynthesis ofthujaplicin and monoterpenes, as well as the catabolism ofthujaplicin by methylation and oxidation [285,279].…”

Section: Production Via Biotechnologymentioning

confidence: 99%

Plant Troponoids: Chemistry, Biological Activity, and Biosynthesis

Zhao

2007

CMC

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104

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Tropone or tropolone and its derivatives (here together called troponoids) belong to a family of natural products with a seven-membered aromatic ring and various side groups. They are mainly synthesized by plants and fungi, and most troponoids play roles of antibacterial defenses in these organisms. With an increasingly severe situation of antibiotic resistant bacteria, as well as a requirement for antifungal medicines, troponoids have attracted extensive studies since they have powerful antibacterial and antifungal activity, particularly against antibiotic-resistant bacteria. In addition, many other biological activities such as antiviral, antitumor, antioxidant, anti-inflammatory, insecticidal, or enzyme inhibitor activities are associated with troponoids. After extensive studies in the 1960s-70s, interests in natural troponoids dedclined. However, chemical and biomedical studies on troponoids bloom again from the 1990s. To date great progress has been made with troponoid study in terms of identification of new natural troponoids, chemical synthesis and properties, biological activity, biosynthesis and metabolism. Particularly, bioassay-guided screening strategy and structure-activity relation-directed structure modification and drug design has resulted in the synthesis and discovery of many new derivatives. Many of them have great promise to be developed into new medicines for their potent and specific activities. This review presents the recent advances in troponoid studies and highlights multiple faceted biological activities of troponoids, as well as their relationships with chemical structures. Chemistry, biosynthesis, and production via biotechnology of troponoids are also briefly reviewed. Applications of troponoids in daily life, agriculture, medicine, and industry, and the related patents have been considered to further extend our understanding of the increasing impact of troponoids on humans.

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“…On the basis of the results obtained in the present study, Monascus purpureus may initiate monacolin K metabolism on day 4. Based on the results above, it could be concluded that after adding the fungal elicitor, the polysaccharides transmitted a signal to the signal receptor of Monascus fungal cells, which triggered a second metabolic reaction when the signal was received by the corresponding enzyme system, thereby producing a large amount of monacolin K (Miyake et al, 2005;Zhao et al, 2006).…”

Section: Discussionmentioning

confidence: 97%

Elevated yield of Monacolin K in Monascus purpureus by fungal elicitor and mutagenesis of UV and LiCl

et al. 2011

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In China, Monascus spp., a traditional fungus used in fermentation, is used as a natural food additive. Monascus spp. can produce a secondary metabolite, monacolin K namely, which is proven to be a cholesterol-lowering and hypotensive agent. Hence, recently, many researchers have begun focusing on how to increase the production of monacolin K by Monascus purpureus. In the present study, we investigated the effect of the fungal elicitor and the mutagenesis of UV & LiCl on the amount of monacolin K produced by Monascus purpureus. The fugal elicitor, Sporobolomyces huaxiensis, was isolated from tea leaves and its fi ltrate was added into the culture fi ltrate of Monascus purpureus during growth to induct the production of monacolin K. The results showed that the highest amount of monacolin K produced by the liquid fermentation was 446.92 mg/mL, which was produced after the fungal elicitor was added to the culture fi ltrate of Monascus purpureus on the day 4; this amount was approximately 6 times greater than that of the control culture fi ltrate, whereas the highest amount of monacolin K produced by the mutated strain was 3 times greater than the control culture after the irradiation of UV light in the presence of 1.0 ‰ LiCl in the medium.

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Signal transduction and metabolic flux of β-thujaplicin and monoterpene biosynthesis in elicited Cupressus lusitanica cell cultures

Cited by 24 publications

References 46 publications

Volatile and non-volatile monoterpenes produced by elicitor-stimulated Cupressus lusitanica cultured cells

Volatile and non-volatile monoterpenes produced by elicitor-stimulated Cupressus lusitanica cultured cells

Plant Troponoids: Chemistry, Biological Activity, and Biosynthesis

Elevated yield of Monacolin K in Monascus purpureus by fungal elicitor and mutagenesis of UV and LiCl

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