Signal-to-noise ratio enhancement of the compact light-emitting diode-induced fluorescence detector

Geng, Xuhui; Wu, Dapeng; Wu, Qian; Guan, Yafeng

doi:10.1016/j.talanta.2012.08.043

Cited by 10 publications

(8 citation statements)

References 16 publications

(28 reference statements)

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“…What was mentioned above for wire-based electrical sensor in principle holds also for optical sensors, where either an optical fiber is implemented into the measurement location, or an active optical component (light emitting diode LED, laser or other light source) is integrated there—in many cases an optical detector like a photo diode or similar could also be used. Examples are presented in references [70,71,72,73,74,75,76].…”

Section: Parameters Measurement Methods and Scalingmentioning

confidence: 99%

In-Situ Measurements in Microscale Gas Flows—Conventional Sensors or Something Else?

Brandner

2019

Micromachines

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Within the last few decades miniaturization has a driving force in almost all areas of technology, leading to a tremendous intensification of systems and processes. Information technology provides now data density several orders of magnitude higher than a few years ago, and the smartphone technology includes, as well the simple ability to communicate with others, features like internet, video and music streaming, but also implementation of the global positioning system, environment sensors or measurement systems for individual health. So-called wearables are everywhere, from the physio-parameter sensing wrist smart watch up to the measurement of heart rates by underwear. This trend holds also for gas flow applications, where complex flow arrangements and measurement systems formerly designed for a macro scale have been transferred into miniaturized versions. Thus, those systems took advantage of the increased surface to volume ratio as well as of the improved heat and mass transfer behavior of miniaturized equipment. In accordance, disadvantages like gas flow mal-distribution on parallelized mini- or micro tubes or channels as well as increased pressure losses due to the minimized hydraulic diameters and an increased roughness-to-dimension ratio have to be taken into account. Furthermore, major problems are arising for measurement and control to be implemented for in-situ and/or in-operando measurements. Currently, correlated measurements are widely discussed to obtain a more comprehensive view to a process by using a broad variety of measurement techniques complementing each other. Techniques for correlated measurements may include commonly used techniques like thermocouples or pressure sensors as well as more complex systems like gas chromatography, mass spectrometry, infrared or ultraviolet spectroscopy and many others. Some of these techniques can be miniaturized, some of them cannot yet. Those should, nevertheless, be able to conduct measurements at the same location and the same time, preferably in-situ and in-operando. Therefore, combinations of measurement instruments might be necessary, which will provide complementary techniques for accessing local process information. A recently more intensively discussed additional possibility is the application of nuclear magnetic resonance (NMR) systems, which might be useful in combination with other, more conventional measurement techniques. NMR is currently undergoing a tremendous change from large-scale to benchtop measurement systems, and it will most likely be further miniaturized. NMR allows a multitude of different measurements, which are normally covered by several instruments. Additionally, NMR can be combined very well with other measurement equipment to perform correlative in-situ and in-operando measurements. Such combinations of several instruments would allow us to retrieve an “information cloud” of a process. This paper will present a view of some common measurement techniques and the difficulties of applying them on one hand in a miniaturized scale, and on the other hand in a correlative mode. Basic suggestions to achieve the above-mentioned objective by a combination of different methods including NMR will be given.

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Section: Parameters Measurement Methods and Scalingmentioning

confidence: 99%

In-Situ Measurements in Microscale Gas Flows—Conventional Sensors or Something Else?

Brandner

2019

Micromachines

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“…3,4 The background signal of uorescence generated with a high voltage light source (e.g., xenon, laser) is typically higher than with chemiluminescence detection which does not require a light source. 6,7 Thus, it is difficult to detect trace levels of double strand DNA (dsDNA)-conjugated uorescent dye without such a long incubation/hybridization time. 6 Unfortunately, the incubation time needed to hybridize mutated DNA with a complementary probe-conjugated uorescent quantum dot (QD655) is as long as 1½ hours because the limit of detection (LOD) of LED uorescence is higher than with conventional uorescence detection.…”

Section: Introductionmentioning

confidence: 99%

“…5 To solve some problems associated with conventional light sources, more cost-effective hybridization methods using uorescence generated by light-emitting diodes (LED) with low energy consumption have been developed. 7 To develop simple hybridization methods with uorescence detection, various nanoparticles (e.g., carbon nanotubes, graphene oxide) capable of capturing the remaining complementary probes aer hybridization of target DNA and complementary probe have been used. 6,7 Thus, it is difficult to detect trace levels of double strand DNA (dsDNA)-conjugated uorescent dye without such a long incubation/hybridization time.…”

Section: Introductionmentioning

confidence: 99%

“…6 Unfortunately, the incubation time needed to hybridize mutated DNA with a complementary probe-conjugated uorescent quantum dot (QD655) is as long as 1½ hours because the limit of detection (LOD) of LED uorescence is higher than with conventional uorescence detection. 6,7 Thus, it is difficult to detect trace levels of double strand DNA (dsDNA)-conjugated uorescent dye without such a long incubation/hybridization time. 7 To develop simple hybridization methods with uorescence detection, various nanoparticles (e.g., carbon nanotubes, graphene oxide) capable of capturing the remaining complementary probes aer hybridization of target DNA and complementary probe have been used.…”

Section: Introductionmentioning

confidence: 99%

“…6,7 Thus, it is difficult to detect trace levels of double strand DNA (dsDNA)-conjugated uorescent dye without such a long incubation/hybridization time. 7 To develop simple hybridization methods with uorescence detection, various nanoparticles (e.g., carbon nanotubes, graphene oxide) capable of capturing the remaining complementary probes aer hybridization of target DNA and complementary probe have been used. 8,9 The complementary probe-conjugated uorescent dye that is immobilized on the nanoparticle surface cannot emit uorescence due to uorescence resonance energy transfer from the dye to the nanoparticle.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Rapid hybridization using graphene oxide and 1,1′-oxalyldiimidazole chemiluminescence

Choi¹,

Choi

Lee³

2013

RSC Adv.

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A rapid hybridization method using graphene oxide and highly sensitive 1,1 0 -oxalyldiimidazole chemiluminescence (ODI-CL) detection was developed from an understanding of the chemical and physical interactions between single strand DNA (ssDNA) molecules and nanoparticles (e.g., multi-walled carbon nanotubes, graphene oxide, gold and silver nanoparticles). The efficiency of hybridization between mutated ssDNA and a complementary probe conjugated with TEX615 was dependent on four different variables: pH, temperature, incubation time, and properties of nanoparticles capable of capturing excess complementary probes remaining after the hybridization. A critical problem observed when mutated ssDNAs rapidly bound with complementary probe-conjugated TEX615 was that three different types of possible mismatched ssDNAs also slowly and competitively hybridized with complementary probe-conjugated TEX615. The problem was solved upon addition of three different types of complementary probes, not conjugated with TEX615, in the solution because mismatched DNAs hybridize with their complementary probes while mutated ssDNAs bind with complementary probe-conjugated TEX615. The new hybridization method using graphene oxide and ODI-CL detection quantified trace levels of mutated ssDNAs in a sample containing mismatched ssDNAs within 15 minutes without any interference from mismatched ssDNAs.

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A novel HPLC flow cell integrated UV light emitting diode induced fluorescence detector as alternative for sensitive determination of aflatoxins

Geng

Wang

Gao

et al. 2018

Analytica Chimica Acta

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Signal-to-noise ratio enhancement of the compact light-emitting diode-induced fluorescence detector

Cited by 10 publications

References 16 publications

In-Situ Measurements in Microscale Gas Flows—Conventional Sensors or Something Else?

In-Situ Measurements in Microscale Gas Flows—Conventional Sensors or Something Else?

Rapid hybridization using graphene oxide and 1,1′-oxalyldiimidazole chemiluminescence

A novel HPLC flow cell integrated UV light emitting diode induced fluorescence detector as alternative for sensitive determination of aflatoxins

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