A novel HPLC flow cell integrated UV light emitting diode induced fluorescence detector as alternative for sensitive determination of aflatoxins

Geng, Xuhui; Wang, Nan; Gao, Yan; Ning, Haijing; Guan, Yafeng

doi:10.1016/j.aca.2018.06.059

Cited by 20 publications

(6 citation statements)

References 19 publications

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“…In regard to biosensors, the minimum detection limit is increased by about 10,000 times [25]. Moreover, our method is more environmentally-friendly and less expensive than HPLC [19]. The detection of CIT in Monascus -rice or Monascus products requires a maximum dilution of about 180-fold, and the LOD level is 20 ng/mL [33].…”

Section: Discussionmentioning

confidence: 99%

“…The detection capabilities of HPLC vary depending on the type of sample, the method of pretreatment, and the type of detector. At present, mainstream detectors include ultraviolet detectors, fluorescence detectors, photodiode array detectors, and mass spectrometer detectors [19,20,21,22]. Biosensors are mainly composed of biological components and signal converters, which can amplify and convert biological signals of target molecules into electrical signals or other signals for analysis and detection purposes.…”

Section: Introductionmentioning

confidence: 99%

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Development of Real-Time Immuno-PCR Based on Phage Displayed an Anti-Idiotypic Nanobody for Quantitative Determination of Citrinin in Monascus

Huang

Ning

et al. 2019

Toxins

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Citrinin (CIT) is a mycotoxin that has been detected in agricultural products, feedstuff, and Monascus products. At present, research has been performed to develop methods for CIT detection, mainly through TLC, HPLC, biosensor, and immunoassay. The immunoassay method is popular with researchers because of its speed, economy, simplicity, and ease of control. However, mycotoxins are inevitably introduced during the determination. Immunoassays require the use of toxins coupled to carrier proteins or enzymes to make competitive antigens. In this study, anti-idiotypic nanobody X27 as CIT mimetic antigen was used as non-toxic surrogate reagents in immunoassay. Therefore, the X27-based real-time immuno-PCR (rtIPCR) method had been established after optimal experiments of annealing temperature and amplification efficiency of real-time PCR, concentration of coating antibody, phage X27, and methyl alcohol. The IC50 value of the established method in the present study is 9.86 ± 2.52 ng/mL, which is nearly equivalent to the traditional phage ELISA method. However, the linear range is of 0.1–1000 ng/mL, which has been broadened 10-fold compared to the phage ELISA method. Besides, the X27-based rtIPCR method has no cross-reactivity to the common mycotoxins, like aflatoxin B1 (AFB1), deoxynivalenol (DON), ochratoxin A (OTA), and zearalenone (ZEN). The method has also been applied to the determination of CIT in rice flour and flour samples, and the recovery was found to be in the range of 90.0–104.6% and 75.8–110.0% respectively. There was no significant difference in the results between the rtIPCR and UPLC–MS. The anti-idiotypic nanobody as a non-toxic surrogate of CIT makes rtIPCR a promising method for actual CIT analysis in Monascus products.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of Real-Time Immuno-PCR Based on Phage Displayed an Anti-Idiotypic Nanobody for Quantitative Determination of Citrinin in Monascus

Huang

Ning

et al. 2019

Toxins

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“…The relationship between the numerical aperture (NA) of the objective, the solid angle (ω), and the collection angel (θ) are shown in eqs and Calculation results from these equations showed that the collection angel of 40× objective (NA = 0.55, ω = 0.33π) was 5.16× bigger than that of 10× objective (NA = 0.25, ω = 0.064π).…”

Section: Results and Discussionmentioning

confidence: 99%

“…Fluorescence in the detection cell was emitted spherically, only a small portion of which can be collected by the microscope objective . In order to improve the collection efficiency, a flat-concave spherical reflector (diameter 4 mm, EFL 6.3 mm) was set face to the objective.…”

Section: Results and Discussionmentioning

confidence: 99%

“…Fluorescence in the detection cell was emitted spherically, only a small portion of which can be collected by the microscope objective. 21 In order to improve the collection efficiency, a flat-concave spherical reflector (diameter 4 mm, EFL 6.3 mm) was set face to the objective. A specially designed dichroic layer (high reflectance at wavelength from 510 to 700 nm, high transmission at wavelength from 400 to 490 nm) was plated on the concave spherical surface, allowing the laser beam passing through and reflecting the fluorescence.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Spherical Dichroic Reflector Improves Limit of Detection in Laser-Induced Fluorescence Detection

Shi

Geng

et al. 2020

Anal. Chem.

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A miniature laser-induced fluorescence (mLIF) detector utilizing a novel spherical dichroic reflector (SDR), an unconventional long working distance high magnification objective, an uncommon broadband emission-matched excitation filter pair, and a silicon-based photodiode detector assembly instead of a photomultiplier tube was developed and evaluated. The detection cell was placed at the spherical center of the SDR instead of the regular focus, yielding a 1.8× signal-to-noise ratio (SNR) improvement. Different from previous works, the use of a 40× objective with a long working distance of 5.38 mm and a broadband BP 527–70 nm emission filter with matched BP 450–30 nm excitation filter improved SNR to 4.6× and 1.9×, respectively. By flow injection analysis (FIA) evaluation, the limit of detection (LOD; 3σ method) for fluorescein sodium was 1.5 × 10–13 M or 8.9 fluorescein molecules in 98 pL detection volume, which was the lowest level of LIFs evaluated by FIA mode. The analysis of three kinds of amino acids with LODs at sub pM to fM level (the lowest levels, hundreds of times lower than previous works using normal capillary) demonstrated the potential of the mLIF in ultratrace analysis of biological and environmental samples, including low copy molecules in a single cell.

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A highly sensitive fluorescence biosensor for aflatoxins B1 detection based on polydiacetylene liposomes combined with exonuclease III-assisted recycling amplification

Tao,

Wang,

Zhu

et al. 2024

Microchim Acta

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A novel HPLC flow cell integrated UV light emitting diode induced fluorescence detector as alternative for sensitive determination of aflatoxins

Cited by 20 publications

References 19 publications

Development of Real-Time Immuno-PCR Based on Phage Displayed an Anti-Idiotypic Nanobody for Quantitative Determination of Citrinin in Monascus

Development of Real-Time Immuno-PCR Based on Phage Displayed an Anti-Idiotypic Nanobody for Quantitative Determination of Citrinin in Monascus

Spherical Dichroic Reflector Improves Limit of Detection in Laser-Induced Fluorescence Detection

A highly sensitive fluorescence biosensor for aflatoxins B1 detection based on polydiacetylene liposomes combined with exonuclease III-assisted recycling amplification

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