Siderophore Producing Aspergillus spp as Bioinoculant for Enhanced Growth of Mung Bean

Abstract

Two fungal species A. niger and A. parasiticus isolated from agriculture field were used in the present study. These two fungal isolates were studied for the siderophore production on the modified CAS agar medium. On daily observation it was observed that the growth was increased along with the production indicating the change in colour of CAS dye from blue to purplish red by A. niger and Yellow by A. parasiticus. Quantitative determination of siderophore production was further studied using liquid medium. After 10 days of incubation A. niger and A. parasiticus produce 80% and 73% units of siderophore. Chemical determination showed that both the fungal isolates showed hydroxamate type of siderophore. Optimization of type of inoculum showed that block inoculum produced maximum siderophore compared to loop and monosporic inoculation in both the fungal isolates. Neutral pH 7.0 showed maximum siderophore production in both the isolates. Decrease in iron concentration increases the siderophore production by the fungal isolates. Maximum siderophore production 80% and 74% units was observed in A. niger and A. parasiticus respectively when there was no addition of iron in the medium. Pot studies were carried out to observe the effect of fungal inoculum on mungbean plant. Inoculation with fungal isolates showed increase in number of leaves, number of branches, number of lateral roots, plant height, fresh weight and dry weight of plant compared to control. Thus both the fungal isolates proved to be potential bioinoculant for improving the growth of mungbean plant.

Siderophore Production by Rhizosphere Inhabiting Bacteria and Fungi

“…In the same test for the siderophore of Aspergillus niger MH603592, its show maximum absorbance at 450 which is confirmed the hydroxymate nature of siderophores (Fig. 4-II) and is in accordance with the report of (Patel et al, 2017), Moreover, (Neilands, 1981) have observed that the iron hydroxamate complex is more stable and therefore more significant in the rhizosphere. While no evidence for carboxylate in the spectrophotometric test for the both isolates.…”

Multifarious functional traits of free-living rhizospheric fungi, with special reference to Aspergillus spp. isolated from North Indian soil, and their inoculation effect on plant growth

“…Aspergillus spp. is known to produce hydroxamate-like siderophores (Haas 2014;Patel et al 2017). During this study, siderophore production was noted in eight Aspergillus isolates followed by the isolates of Curvularia, Alternaria, Monotospora, Penicillium, Trichoderma, Chlamydospora, Mucor, and Trichothecium.…”

Synergistic Effect of Azotobacter nigricans and Nitrogen Phosphorus Potassium Fertilizer on Agronomic and Yieldtraits of Maize (Zea mays L.)

“… Ritika and Utpal (2014) showed in their review that the use of Azotobacter as an N-biofertilizer increased the growth and yield of various crops under field conditions with a percentage increase of up to 40% for Cauliflower and 15–20% for Maize compared to conventional fertilizers. These beneficial effects can be attributed to the biosynthesis of biologically active substances, the stimulation of rhizospheric microorganisms, the production of phytopathogen inhibitors, and improved nutrient availability of N, P, carbon, and sulfur, through BNF and mineralization of organic residues in soil ( Lenart, 2012 ; Patel et al, 2017 ). Numerous studies described crop responses to Azotobacter inoculation under greenhouse and field conditions.…”